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Updating strategies for isolating and discovering giant viruses.
Curr Opin Microbiol. 2016 06; 31:80-87.CO

Abstract

Almost fifteen years ago, the discovery of Acanthamoeba polyphaga mimivirus, the first giant virus, changed how we define a virus. It was discovered incidentally in a process of isolating Legionella sp. from environmental samples in the context of pneumonia epidemics using a co-culture system with Acanthamoeba. Since then, much effort and improvement has been put into the original technique. In addition to the known families of Mimiviridae and Marseilleviridae, four new proposed families of giant viruses have been isolated: Pandoravirus, Pithovirus, Faustovirus and Mollivirus. Major improvements were based on enrichment systems, targeted use of antibiotics and high-throughput methods. The most recent development, using flow cytometry for isolation and presumptive identification systems, opens a path to large environmental surveys that may discover new giant virus families in new protozoa supports used for culture support.

Authors+Show Affiliations

Aix-Marseille Univ., Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE), UM63 CNRS 7278 IRD 198 INSERM U1095, Facultés de Médecine et de Pharmacie, Marseille, France.Aix-Marseille Univ., Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE), UM63 CNRS 7278 IRD 198 INSERM U1095, Facultés de Médecine et de Pharmacie, Marseille, France.Aix-Marseille Univ., Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE), UM63 CNRS 7278 IRD 198 INSERM U1095, Facultés de Médecine et de Pharmacie, Marseille, France; Institut Hospitalo-Universitaire (IHU) Méditerranée Infection, Pôle des Maladies Infectieuses et Tropicales Clinique et Biologique, Fédération de Bactériologie-Hygiène-Virologie, Centre Hospitalo-Universitaire Timone, Assistance Publique - Hôpitaux de Marseille, Marseille, France. Electronic address: bernard.la-scola@univ-amu.fr.

Pub Type(s)

Journal Article
Review

Language

eng

PubMed ID

27039269

Citation

Khalil, Jacques Yaacoub Bou, et al. "Updating Strategies for Isolating and Discovering Giant Viruses." Current Opinion in Microbiology, vol. 31, 2016, pp. 80-87.
Khalil JYB, Andreani J, La Scola B. Updating strategies for isolating and discovering giant viruses. Curr Opin Microbiol. 2016;31:80-87.
Khalil, J. Y. B., Andreani, J., & La Scola, B. (2016). Updating strategies for isolating and discovering giant viruses. Current Opinion in Microbiology, 31, 80-87. https://doi.org/10.1016/j.mib.2016.03.004
Khalil JYB, Andreani J, La Scola B. Updating Strategies for Isolating and Discovering Giant Viruses. Curr Opin Microbiol. 2016;31:80-87. PubMed PMID: 27039269.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Updating strategies for isolating and discovering giant viruses. AU - Khalil,Jacques Yaacoub Bou, AU - Andreani,Julien, AU - La Scola,Bernard, Y1 - 2016/04/01/ PY - 2016/02/16/received PY - 2016/03/14/revised PY - 2016/03/18/accepted PY - 2016/4/4/entrez PY - 2016/4/4/pubmed PY - 2017/11/29/medline SP - 80 EP - 87 JF - Current opinion in microbiology JO - Curr Opin Microbiol VL - 31 N2 - Almost fifteen years ago, the discovery of Acanthamoeba polyphaga mimivirus, the first giant virus, changed how we define a virus. It was discovered incidentally in a process of isolating Legionella sp. from environmental samples in the context of pneumonia epidemics using a co-culture system with Acanthamoeba. Since then, much effort and improvement has been put into the original technique. In addition to the known families of Mimiviridae and Marseilleviridae, four new proposed families of giant viruses have been isolated: Pandoravirus, Pithovirus, Faustovirus and Mollivirus. Major improvements were based on enrichment systems, targeted use of antibiotics and high-throughput methods. The most recent development, using flow cytometry for isolation and presumptive identification systems, opens a path to large environmental surveys that may discover new giant virus families in new protozoa supports used for culture support. SN - 1879-0364 UR - https://www.unboundmedicine.com/medline/citation/27039269/Updating_strategies_for_isolating_and_discovering_giant_viruses_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1369-5274(16)30019-4 DB - PRIME DP - Unbound Medicine ER -