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Vitamin D receptor gene polymorphisms/haplotypes and serum 25(OH)D3 levels in Hashimoto's thyroiditis.
Endocrine. 2017 Feb; 55(2):599-606.E

Abstract

Vitamin D deficiency and/or reduced function, as per certain polymorphisms of the vitamin D receptor (VDR) gene, have been related to several autoimmune disorders. The present study was aimed to investigate the association of Hashimoto's thyroiditis with vitamin D status and functional polymorphisms (SNPs) of the VDR gene. In this case-control study, 200 euthyroid subjects were enrolled: 100 newly diagnosed HT patients (87 F, 13 M; mean age ± SD 42 ± 15 year) and 100 healthy individuals, matched for age, sex, BMI, and month of blood sampling. Serum 25(OH)D3 was measured by HPLC. The VDR SNPs BsmI, ApaI, and TaqI, in strong linkage disequilibrium with each other, were detected by restriction fragment length polymorphism-PCR. The prevalence of vitamin D deficiency in HT patients was significantly higher than that in the control group (70 vs 18.2 %; p < 0.0001), and median serum 25(OH)D3 level was significantly lower in HT patients than controls (median value: 16.2 vs 37.4 ng/ml; p = 0.026). Moreover, there was a significant inverse correlation between serum 25(OH)D3 and TPOAb concentration (r = -0.669; p = 0.034). Contrarily, the genotype distribution of the studied SNPs was not different in the two groups (BsmI p = 0.783; ApaI p = 0.512; TaqI p = 0.471), as was the allelic frequency [f(B) p = 0.776, f(b) p = 0.887; f(A) p = 0.999, f(a) p = 0.999; f(T) p = 0.617; f(t) p = 0.617]. The present study first investigates newly diagnosed untreated HT and suggests that vitamin D deficiency may contribute to HT development and/or progression, acting as an environmental trigger, while the VDR locus does not appear to be involved in conditioning the genetic susceptibility to the disease, at least in Caucasians.

Authors+Show Affiliations

Unit of Endocrinology, Department of Clinical and Experimental Medicine, University of Messina, AOU Policlinico "G. Martino" (Pad H, Floor 4), Via Consolare Valeria, 1, 98125, Messina, Italy.Unit of Endocrinology, Department of Clinical and Experimental Medicine, University of Messina, AOU Policlinico "G. Martino" (Pad H, Floor 4), Via Consolare Valeria, 1, 98125, Messina, Italy.Unit of Endocrinology, Department of Clinical and Experimental Medicine, University of Messina, AOU Policlinico "G. Martino" (Pad H, Floor 4), Via Consolare Valeria, 1, 98125, Messina, Italy.Department of Economics, University of Messina, Messina, Italy.Casa Sollievo Sofferenza Hospital, IRCCS, San Giovanni Rotondo, Italy.Department of Biomedical Sciences and Morpho-Functional Imaging, University of Messina, Messina, Italy.Unit of Endocrinology, Department of Clinical and Experimental Medicine, University of Messina, AOU Policlinico "G. Martino" (Pad H, Floor 4), Via Consolare Valeria, 1, 98125, Messina, Italy.Unit of Endocrinology, Department of Clinical and Experimental Medicine, University of Messina, AOU Policlinico "G. Martino" (Pad H, Floor 4), Via Consolare Valeria, 1, 98125, Messina, Italy. Accademia Peloritana dei Pericolanti, University of Messina, Messina, Italy.Unit of Endocrinology, Department of Clinical and Experimental Medicine, University of Messina, AOU Policlinico "G. Martino" (Pad H, Floor 4), Via Consolare Valeria, 1, 98125, Messina, Italy. rmruggeri@unime.it.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27043843

Citation

Giovinazzo, Salvatore, et al. "Vitamin D Receptor Gene Polymorphisms/haplotypes and Serum 25(OH)D3 Levels in Hashimoto's Thyroiditis." Endocrine, vol. 55, no. 2, 2017, pp. 599-606.
Giovinazzo S, Vicchio TM, Certo R, et al. Vitamin D receptor gene polymorphisms/haplotypes and serum 25(OH)D3 levels in Hashimoto's thyroiditis. Endocrine. 2017;55(2):599-606.
Giovinazzo, S., Vicchio, T. M., Certo, R., Alibrandi, A., Palmieri, O., Campennì, A., Cannavò, S., Trimarchi, F., & Ruggeri, R. M. (2017). Vitamin D receptor gene polymorphisms/haplotypes and serum 25(OH)D3 levels in Hashimoto's thyroiditis. Endocrine, 55(2), 599-606. https://doi.org/10.1007/s12020-016-0942-5
Giovinazzo S, et al. Vitamin D Receptor Gene Polymorphisms/haplotypes and Serum 25(OH)D3 Levels in Hashimoto's Thyroiditis. Endocrine. 2017;55(2):599-606. PubMed PMID: 27043843.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Vitamin D receptor gene polymorphisms/haplotypes and serum 25(OH)D3 levels in Hashimoto's thyroiditis. AU - Giovinazzo,Salvatore, AU - Vicchio,Teresa M, AU - Certo,Rosaria, AU - Alibrandi,Angela, AU - Palmieri,Orazio, AU - Campennì,Alfredo, AU - Cannavò,Salvatore, AU - Trimarchi,Francesco, AU - Ruggeri,Rosaria Maddalena, Y1 - 2016/04/04/ PY - 2016/01/31/received PY - 2016/03/24/accepted PY - 2016/4/5/pubmed PY - 2017/7/27/medline PY - 2016/4/5/entrez KW - Autoimmunity KW - Hashimoto’s thyroiditis KW - Single nucleotide polymorphisms KW - Vitamin D KW - Vitamin D receptor gene (VDR) SP - 599 EP - 606 JF - Endocrine JO - Endocrine VL - 55 IS - 2 N2 - Vitamin D deficiency and/or reduced function, as per certain polymorphisms of the vitamin D receptor (VDR) gene, have been related to several autoimmune disorders. The present study was aimed to investigate the association of Hashimoto's thyroiditis with vitamin D status and functional polymorphisms (SNPs) of the VDR gene. In this case-control study, 200 euthyroid subjects were enrolled: 100 newly diagnosed HT patients (87 F, 13 M; mean age ± SD 42 ± 15 year) and 100 healthy individuals, matched for age, sex, BMI, and month of blood sampling. Serum 25(OH)D3 was measured by HPLC. The VDR SNPs BsmI, ApaI, and TaqI, in strong linkage disequilibrium with each other, were detected by restriction fragment length polymorphism-PCR. The prevalence of vitamin D deficiency in HT patients was significantly higher than that in the control group (70 vs 18.2 %; p < 0.0001), and median serum 25(OH)D3 level was significantly lower in HT patients than controls (median value: 16.2 vs 37.4 ng/ml; p = 0.026). Moreover, there was a significant inverse correlation between serum 25(OH)D3 and TPOAb concentration (r = -0.669; p = 0.034). Contrarily, the genotype distribution of the studied SNPs was not different in the two groups (BsmI p = 0.783; ApaI p = 0.512; TaqI p = 0.471), as was the allelic frequency [f(B) p = 0.776, f(b) p = 0.887; f(A) p = 0.999, f(a) p = 0.999; f(T) p = 0.617; f(t) p = 0.617]. The present study first investigates newly diagnosed untreated HT and suggests that vitamin D deficiency may contribute to HT development and/or progression, acting as an environmental trigger, while the VDR locus does not appear to be involved in conditioning the genetic susceptibility to the disease, at least in Caucasians. SN - 1559-0100 UR - https://www.unboundmedicine.com/medline/citation/27043843/Vitamin_D_receptor_gene_polymorphisms/haplotypes_and_serum_25_OH_D3_levels_in_Hashimoto's_thyroiditis_ L2 - https://dx.doi.org/10.1007/s12020-016-0942-5 DB - PRIME DP - Unbound Medicine ER -