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Inhibitory effect of baicalin on allergic response in ovalbumin-induced allergic rhinitis guinea pigs and lipopolysaccharide-stimulated human mast cells.
Inflamm Res 2016; 65(8):603-12IR

Abstract

OBJECTIVE

Baicalin, a flavonoid compound purified from the dry roots of Scutellaria baicalensis Georgi, has generally been used for the treatment of various allergic diseases. However, there is little information about the anti-inflammatory effects of baicalin for allergic rhinitis. This study aims to investigate the anti-allergic effect of baicalin on allergic response in ovalbumin (OVA)-induced allergic rhinitis guinea pigs and lipopolysaccharide (LPS)-stimulated human mast cells.

METHODS

Using in vivo models, we evaluated the effect of baicalin on allergic rhinitis symptoms via recording the number of nasal rubs and sneezes. The levels of histamine, OVA-specific immunoglobulin E(IgE), eosinophil cationic protein (ECP) and inflammatory cytokines were measured using enzyme-linked immunosorbent assay (ELISA). The histological changes of nasal mucosa were observed by light microscope after HE staining. In vitro, the release of histamine and β-hexosaminidase of compound 48/80-induced human mast cells were measured by ELISA and PNP-NAG colorimetry, respectively. The productions of inflammatory cytokines of LPS-stimulated human mast cells were determined using ELISA. Western blot was used to test the protein expression of JAK2, p-JAK2, STAT5, p-STAT5, IKKβ, p-IKKβ, IκBα, p-IκBα and NF-κB (p65) of LPS-stimulated human mast cells.

RESULTS

The oral administration of baicalin at doses of 50 and 200 mg/kg improved allergic rhinitis symptoms and the histological changes of nasal mucosa and decreased the serum levels of histamine, ECP, interleukin (IL)-1β, IL-6, IL-8, tumor necrosis factor (TNF)-α and OVA-specific IgE in OVA-induced allergic rhinitis guinea pigs. In vitro, baicalin suppressed the release of histamine and β-hexosaminidase in compound 48/80-induced human mast cells. In addition, baicalin also inhibited the productions of inflammatory cytokines such as IL-1β, IL-6, IL-8 and TNF-α and suppressed the phosphorylation of JAK2, STAT5, IKKβ, IκBα and the nuclear translocation of NF-κB (p65) subunit in LPS-stimulated human mast cells.

CONCLUSIONS

These results suggest that baicalin can effectively prevent allergic response in OVA-induced allergic rhinitis guinea pigs and inhibit inflammatory response via blocking JAK2-STAT5 and NF-κB signaling pathways in LPS-stimulated human mast cells. Considered together,the results show that baicalin may be a useful drug in the treatment of allergic rhinitis.

Authors+Show Affiliations

Department of Pharmacology, Chongqing Medical University, Chongqing, 400016, China. Chongqing Key Laboratory of Biochemistry and Molecular Pharmacology, Chongqing, 400016, China.Department of Pharmacology, Chongqing Medical University, Chongqing, 400016, China.Department of Pharmacology, Chongqing Medical University, Chongqing, 400016, China.Department of Pharmacology, Chongqing Medical University, Chongqing, 400016, China.Jingzhou Institute of Technology, Jingzhou, 434020, Hubei, China.Department of Pharmacology, Chongqing Medical University, Chongqing, 400016, China. huangjiajuncqmu@163.com. Chongqing Key Laboratory of Biochemistry and Molecular Pharmacology, Chongqing, 400016, China. huangjiajuncqmu@163.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27043920

Citation

Zhou, Yun-Jiang, et al. "Inhibitory Effect of Baicalin On Allergic Response in Ovalbumin-induced Allergic Rhinitis Guinea Pigs and Lipopolysaccharide-stimulated Human Mast Cells." Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.], vol. 65, no. 8, 2016, pp. 603-12.
Zhou YJ, Wang H, Sui HH, et al. Inhibitory effect of baicalin on allergic response in ovalbumin-induced allergic rhinitis guinea pigs and lipopolysaccharide-stimulated human mast cells. Inflamm Res. 2016;65(8):603-12.
Zhou, Y. J., Wang, H., Sui, H. H., Li, L., Zhou, C. L., & Huang, J. J. (2016). Inhibitory effect of baicalin on allergic response in ovalbumin-induced allergic rhinitis guinea pigs and lipopolysaccharide-stimulated human mast cells. Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.], 65(8), pp. 603-12. doi:10.1007/s00011-016-0943-0.
Zhou YJ, et al. Inhibitory Effect of Baicalin On Allergic Response in Ovalbumin-induced Allergic Rhinitis Guinea Pigs and Lipopolysaccharide-stimulated Human Mast Cells. Inflamm Res. 2016;65(8):603-12. PubMed PMID: 27043920.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibitory effect of baicalin on allergic response in ovalbumin-induced allergic rhinitis guinea pigs and lipopolysaccharide-stimulated human mast cells. AU - Zhou,Yun-Jiang, AU - Wang,Hu, AU - Sui,He-Huan, AU - Li,Li, AU - Zhou,Chun-Ling, AU - Huang,Jia-Jun, Y1 - 2016/04/04/ PY - 2015/11/17/received PY - 2016/03/25/accepted PY - 2016/03/23/revised PY - 2016/4/5/entrez PY - 2016/4/5/pubmed PY - 2017/4/14/medline KW - Allergic rhinitis KW - Baicalin KW - Human mast cells KW - Lipopolysaccharide KW - Ovalbumin SP - 603 EP - 12 JF - Inflammation research : official journal of the European Histamine Research Society ... [et al.] JO - Inflamm. Res. VL - 65 IS - 8 N2 - OBJECTIVE: Baicalin, a flavonoid compound purified from the dry roots of Scutellaria baicalensis Georgi, has generally been used for the treatment of various allergic diseases. However, there is little information about the anti-inflammatory effects of baicalin for allergic rhinitis. This study aims to investigate the anti-allergic effect of baicalin on allergic response in ovalbumin (OVA)-induced allergic rhinitis guinea pigs and lipopolysaccharide (LPS)-stimulated human mast cells. METHODS: Using in vivo models, we evaluated the effect of baicalin on allergic rhinitis symptoms via recording the number of nasal rubs and sneezes. The levels of histamine, OVA-specific immunoglobulin E(IgE), eosinophil cationic protein (ECP) and inflammatory cytokines were measured using enzyme-linked immunosorbent assay (ELISA). The histological changes of nasal mucosa were observed by light microscope after HE staining. In vitro, the release of histamine and β-hexosaminidase of compound 48/80-induced human mast cells were measured by ELISA and PNP-NAG colorimetry, respectively. The productions of inflammatory cytokines of LPS-stimulated human mast cells were determined using ELISA. Western blot was used to test the protein expression of JAK2, p-JAK2, STAT5, p-STAT5, IKKβ, p-IKKβ, IκBα, p-IκBα and NF-κB (p65) of LPS-stimulated human mast cells. RESULTS: The oral administration of baicalin at doses of 50 and 200 mg/kg improved allergic rhinitis symptoms and the histological changes of nasal mucosa and decreased the serum levels of histamine, ECP, interleukin (IL)-1β, IL-6, IL-8, tumor necrosis factor (TNF)-α and OVA-specific IgE in OVA-induced allergic rhinitis guinea pigs. In vitro, baicalin suppressed the release of histamine and β-hexosaminidase in compound 48/80-induced human mast cells. In addition, baicalin also inhibited the productions of inflammatory cytokines such as IL-1β, IL-6, IL-8 and TNF-α and suppressed the phosphorylation of JAK2, STAT5, IKKβ, IκBα and the nuclear translocation of NF-κB (p65) subunit in LPS-stimulated human mast cells. CONCLUSIONS: These results suggest that baicalin can effectively prevent allergic response in OVA-induced allergic rhinitis guinea pigs and inhibit inflammatory response via blocking JAK2-STAT5 and NF-κB signaling pathways in LPS-stimulated human mast cells. Considered together,the results show that baicalin may be a useful drug in the treatment of allergic rhinitis. SN - 1420-908X UR - https://www.unboundmedicine.com/medline/citation/27043920/Inhibitory_effect_of_baicalin_on_allergic_response_in_ovalbumin_induced_allergic_rhinitis_guinea_pigs_and_lipopolysaccharide_stimulated_human_mast_cells_ L2 - https://dx.doi.org/10.1007/s00011-016-0943-0 DB - PRIME DP - Unbound Medicine ER -