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Adipose-derived mesenchymal stromal (stem) cells differentiate to osteoblast and chondroblast lineages upon incubation with conditioned media from dental pulp stem cell-derived osteoblasts and auricle cartilage chondrocytes.
J Biol Regul Homeost Agents 2016 Jan-Mar; 30(1):111-22JB

Abstract

The potential of adipose-derived mesenchymal stromal (stem) cells (ADSCs) to differentiate into either osteoblasts or chondrocytes is controversial. In this study we investigated the multicapacity potential of ADSCs to differentiate towards adipocyte, osteoblast, and chondrocyte lineages when cells are seeded onto plastic in comparison with incubation with conditioned media (CM) obtained from differentiated cell types.ADSCs, obtained from liposuctions, were characterized for mesenchymal and hematopoietic markers by cytofluorimetry. Their differentiation capacity towards adipocytes, osteoblasts, and chondrocytes was investigated by histochemistry methods (Oil-Red-O staining, Safranin O and Alizarin Red staining, respectively). Dental pulp stem cells (DPSCs) and dedifferentiated auricle derived-chondrocytes were differentiated towards osteoblastic and chondrocytic lineages respectively, and the CM obtained from these cultures was used to induce differentiation of ADSCs. ADSCs were positive for mesenchymal markers (CD29, CD105, CD73, CD44), but not for hematopoietic lineage markers (CD14, CD34, CD45) and this behavior was conserved from the isolation up to the fifth passage. While ADSCs were readily differentiated in adipocytes, they were not towards chondrocytes and osteoblastic lineages, a behavior different from that of bone marrow-derived MSCs that differentiated into the three lineages at two weeks post-induction. Only ADSCs treated with CM from cultured chondrocytes and DPSCs, produced glycosaminoglycans and mineralized matrix. These results indicate that ADSCs need growth/morphogenic factor supplementation from the tissue environment to be appropriately differentiated to mesodermic lineages.

Authors+Show Affiliations

Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, Medical Genetics Laboratory, Milan, Italy; Department of Medical and Surgical Sciences, University of Foggia, Foggia, Italy.Department of Clinical and Experimental Medicine, University of Foggia, Foggia, Italy.Department of Clinical and Experimental Medicine, University of Foggia, Foggia, Italy.Department of Medical and Surgical Sciences, University of Foggia, Foggia, Italy.Department of Medical and Surgical Sciences, University of Foggia, Foggia, Italy.Department of Clinical and Experimental Medicine, University of Foggia, Foggia, Italy.Department of Clinical and Experimental Medicine, University of Foggia, Foggia, Italy.Department of Medical and Surgical Sciences, University of Foggia, Foggia, Italy.Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, Cystic Fibrosis Center, Milan, Italy.Department of Clinical and Experimental Medicine, University of Foggia, Foggia, Italy.Department of Clinical and Experimental Medicine, University of Foggia, Foggia, Italy.Department of Clinical and Experimental Medicine, University of Foggia, Foggia, Italy.Department of Clinical and Experimental Medicine, University of Foggia, Foggia, Italy.Department of Clinical and Experimental Medicine, University of Foggia, Foggia, Italy.Department of Medical and Surgical Sciences, University of Foggia, Foggia, Italy.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27049081

Citation

Carbone, A, et al. "Adipose-derived Mesenchymal Stromal (stem) Cells Differentiate to Osteoblast and Chondroblast Lineages Upon Incubation With Conditioned Media From Dental Pulp Stem Cell-derived Osteoblasts and Auricle Cartilage Chondrocytes." Journal of Biological Regulators and Homeostatic Agents, vol. 30, no. 1, 2016, pp. 111-22.
Carbone A, Valente M, Annacontini L, et al. Adipose-derived mesenchymal stromal (stem) cells differentiate to osteoblast and chondroblast lineages upon incubation with conditioned media from dental pulp stem cell-derived osteoblasts and auricle cartilage chondrocytes. J Biol Regul Homeost Agents. 2016;30(1):111-22.
Carbone, A., Valente, M., Annacontini, L., Castellani, S., Di Gioia, S., Parisi, D., ... Conese, M. (2016). Adipose-derived mesenchymal stromal (stem) cells differentiate to osteoblast and chondroblast lineages upon incubation with conditioned media from dental pulp stem cell-derived osteoblasts and auricle cartilage chondrocytes. Journal of Biological Regulators and Homeostatic Agents, 30(1), pp. 111-22.
Carbone A, et al. Adipose-derived Mesenchymal Stromal (stem) Cells Differentiate to Osteoblast and Chondroblast Lineages Upon Incubation With Conditioned Media From Dental Pulp Stem Cell-derived Osteoblasts and Auricle Cartilage Chondrocytes. J Biol Regul Homeost Agents. 2016;30(1):111-22. PubMed PMID: 27049081.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Adipose-derived mesenchymal stromal (stem) cells differentiate to osteoblast and chondroblast lineages upon incubation with conditioned media from dental pulp stem cell-derived osteoblasts and auricle cartilage chondrocytes. AU - Carbone,A, AU - Valente,M, AU - Annacontini,L, AU - Castellani,S, AU - Di Gioia,S, AU - Parisi,D, AU - Rucci,M, AU - Belgiovine,G, AU - Colombo,C, AU - Di Benedetto,A, AU - Mori,G, AU - Lo Muzio,L, AU - Maiorella,A, AU - Portincasa,A, AU - Conese,M, PY - 2016/4/7/entrez PY - 2016/4/7/pubmed PY - 2016/7/9/medline SP - 111 EP - 22 JF - Journal of biological regulators and homeostatic agents JO - J. Biol. Regul. Homeost. Agents VL - 30 IS - 1 N2 - The potential of adipose-derived mesenchymal stromal (stem) cells (ADSCs) to differentiate into either osteoblasts or chondrocytes is controversial. In this study we investigated the multicapacity potential of ADSCs to differentiate towards adipocyte, osteoblast, and chondrocyte lineages when cells are seeded onto plastic in comparison with incubation with conditioned media (CM) obtained from differentiated cell types.ADSCs, obtained from liposuctions, were characterized for mesenchymal and hematopoietic markers by cytofluorimetry. Their differentiation capacity towards adipocytes, osteoblasts, and chondrocytes was investigated by histochemistry methods (Oil-Red-O staining, Safranin O and Alizarin Red staining, respectively). Dental pulp stem cells (DPSCs) and dedifferentiated auricle derived-chondrocytes were differentiated towards osteoblastic and chondrocytic lineages respectively, and the CM obtained from these cultures was used to induce differentiation of ADSCs. ADSCs were positive for mesenchymal markers (CD29, CD105, CD73, CD44), but not for hematopoietic lineage markers (CD14, CD34, CD45) and this behavior was conserved from the isolation up to the fifth passage. While ADSCs were readily differentiated in adipocytes, they were not towards chondrocytes and osteoblastic lineages, a behavior different from that of bone marrow-derived MSCs that differentiated into the three lineages at two weeks post-induction. Only ADSCs treated with CM from cultured chondrocytes and DPSCs, produced glycosaminoglycans and mineralized matrix. These results indicate that ADSCs need growth/morphogenic factor supplementation from the tissue environment to be appropriately differentiated to mesodermic lineages. SN - 0393-974X UR - https://www.unboundmedicine.com/medline/citation/27049081/Adipose_derived_mesenchymal_stromal__stem__cells_differentiate_to_osteoblast_and_chondroblast_lineages_upon_incubation_with_conditioned_media_from_dental_pulp_stem_cell_derived_osteoblasts_and_auricle_cartilage_chondrocytes_ DB - PRIME DP - Unbound Medicine ER -