Tags

Type your tag names separated by a space and hit enter

Molecular characterization of carbapenemase genes in Acinetobacter baumannii in China.
Genet Mol Res. 2016 Mar 31; 15(1)GM

Abstract

Acinetobacter baumannii is an aerobic non-motile Gram-negative coccobacillus, and it is one of the most important nosocomial pathogens worldwide. The aim of this study was to determine the molecular epidemiology of the outbreak strains. Between March 2011 and March 2014, a total of 205 strains of A. baumannii were isolated from patients at the Nanyang City Center Hospital. The blaOXA-23, blaOXA-24, blaOXA-51, and blaOXA-58 genes were amplified by multiplex polymerase chain reaction. We found that 68 (33.17%) strains were positive for the blaOXA-23 gene, and 88.24% of these 68 showed resistance to carbapenems, while 11.76% were sensitive to carbapenems. The blaOXA-51 gene was found in 132 (64.39%) strains, and 17.42% of these were resistant to carbapenems while 82.58% were sensitive to carbapenems. Moreover, 5 (2.44%) strains were positive for blaOXA-58, of which 80% were resistant to carbapenems and 20% were sensitive to carbapenems. We found that A. baumannii showed 100% drug resistance to ampicillin, cefotetan, cefazolin, and cefoperazone. Our findings suggest that the blaOXA-23 and blaOXA-51 genes are most frequently identified in A. baumannii, while blaOXA-23 is the most important gene for resistance to carbapenems.

Authors+Show Affiliations

The CT Room, Nanyang City Center Hospital, Nanyang, China.Department of Endocrinology, Nanyang City Center Hospital, Nanyang, China.Department of Nursing, Nanyang City Center Hospital, Nanyang, China.Department of Nursing, Nanyang City Center Hospital, Nanyang, China.Department of Nursing, Nanyang City Center Hospital, Nanyang, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27051026

Citation

Fang, F, et al. "Molecular Characterization of Carbapenemase Genes in Acinetobacter Baumannii in China." Genetics and Molecular Research : GMR, vol. 15, no. 1, 2016.
Fang F, Wang S, Dang YX, et al. Molecular characterization of carbapenemase genes in Acinetobacter baumannii in China. Genet Mol Res. 2016;15(1).
Fang, F., Wang, S., Dang, Y. X., Wang, X., & Yu, G. Q. (2016). Molecular characterization of carbapenemase genes in Acinetobacter baumannii in China. Genetics and Molecular Research : GMR, 15(1). https://doi.org/10.4238/gmr.15017432
Fang F, et al. Molecular Characterization of Carbapenemase Genes in Acinetobacter Baumannii in China. Genet Mol Res. 2016 Mar 31;15(1) PubMed PMID: 27051026.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular characterization of carbapenemase genes in Acinetobacter baumannii in China. AU - Fang,F, AU - Wang,S, AU - Dang,Y X, AU - Wang,X, AU - Yu,G Q, Y1 - 2016/03/31/ PY - 2016/4/7/entrez PY - 2016/4/7/pubmed PY - 2017/1/12/medline JF - Genetics and molecular research : GMR JO - Genet. Mol. Res. VL - 15 IS - 1 N2 - Acinetobacter baumannii is an aerobic non-motile Gram-negative coccobacillus, and it is one of the most important nosocomial pathogens worldwide. The aim of this study was to determine the molecular epidemiology of the outbreak strains. Between March 2011 and March 2014, a total of 205 strains of A. baumannii were isolated from patients at the Nanyang City Center Hospital. The blaOXA-23, blaOXA-24, blaOXA-51, and blaOXA-58 genes were amplified by multiplex polymerase chain reaction. We found that 68 (33.17%) strains were positive for the blaOXA-23 gene, and 88.24% of these 68 showed resistance to carbapenems, while 11.76% were sensitive to carbapenems. The blaOXA-51 gene was found in 132 (64.39%) strains, and 17.42% of these were resistant to carbapenems while 82.58% were sensitive to carbapenems. Moreover, 5 (2.44%) strains were positive for blaOXA-58, of which 80% were resistant to carbapenems and 20% were sensitive to carbapenems. We found that A. baumannii showed 100% drug resistance to ampicillin, cefotetan, cefazolin, and cefoperazone. Our findings suggest that the blaOXA-23 and blaOXA-51 genes are most frequently identified in A. baumannii, while blaOXA-23 is the most important gene for resistance to carbapenems. SN - 1676-5680 UR - https://www.unboundmedicine.com/medline/citation/27051026/Molecular_characterization_of_carbapenemase_genes_in_Acinetobacter_baumannii_in_China L2 - http://www.geneticsmr.com/articles/6199 DB - PRIME DP - Unbound Medicine ER -