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[Effects of YAP-small interfering RNA on the proliferation and apoptosis of human periodontal ligament stem cells].
Hua Xi Kou Qiang Yi Xue Za Zhi. 2015 Dec; 33(6):622-6.HX

Abstract

OBJECTIVE

To investigate the effects of small interfering RNA (siRNA) targeting YAP on the proliferation and apoptosis of human periodontal ligament stem cells (hPDLSCs).

METHODS

Synthesized sequences of siRNA were transfected into hPDLSCs by Lipofectamine™ 2000. The expression of YAP was identified by using real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analysis. Proliferation activity was detected by using cell counting kit-8 (CCK-8). Changes in the cell cycle and apoptosis rate were detected by using flow cytometry. Results were analyzed by using SPSS 19.0, and P < 0.05 was considered statistically significant.

RESULTS

Expression of YAP mRNA and protein were significantly downregulated after 48 h of transfection (P < 0.001). No obvious difference was found in the expression levels of YAP protein between 48 and 72 h, thus indicating that siRNA could inhibit the expression of YAP persistently and effectively. Proliferation activity was inhibited, and apoptosis rate was increased. Cell cycle was changed as the proportion of G₁and S phases increased (P < 0.01) and G₂ phase decreased (P < 0.05).

CONCLUSION

Knocking down YAP gene by siRNA could inhibit proliferation activity, induce apoptosis, and change the cell cycle of hPDLSCs. Thus, YAP could regulate the proliferation and apoptosis of hPDLSCs.

Authors

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Pub Type(s)

Journal Article

Language

chi

PubMed ID

27051957

Citation

Cuizhu, Tang, et al. "[Effects of YAP-small Interfering RNA On the Proliferation and Apoptosis of Human Periodontal Ligament Stem Cells]." Hua Xi Kou Qiang Yi Xue Za Zhi = Huaxi Kouqiang Yixue Zazhi = West China Journal of Stomatology, vol. 33, no. 6, 2015, pp. 622-6.
Cuizhu T, Yong W, Weiting G, et al. [Effects of YAP-small interfering RNA on the proliferation and apoptosis of human periodontal ligament stem cells]. Hua Xi Kou Qiang Yi Xue Za Zhi. 2015;33(6):622-6.
Cuizhu, T., Yong, W., Weiting, G., Bing, Z., Yunpeng, Z., Yawen, J., & Xin, X. (2015). [Effects of YAP-small interfering RNA on the proliferation and apoptosis of human periodontal ligament stem cells]. Hua Xi Kou Qiang Yi Xue Za Zhi = Huaxi Kouqiang Yixue Zazhi = West China Journal of Stomatology, 33(6), 622-6.
Cuizhu T, et al. [Effects of YAP-small Interfering RNA On the Proliferation and Apoptosis of Human Periodontal Ligament Stem Cells]. Hua Xi Kou Qiang Yi Xue Za Zhi. 2015;33(6):622-6. PubMed PMID: 27051957.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effects of YAP-small interfering RNA on the proliferation and apoptosis of human periodontal ligament stem cells]. AU - Cuizhu,Tang, AU - Yong,Wen, AU - Weiting,Gu, AU - Bing,Zhang, AU - Yunpeng,Zhang, AU - Yawen,Ji, AU - Xin,Xu, PY - 2016/4/8/entrez PY - 2016/4/8/pubmed PY - 2017/2/14/medline SP - 622 EP - 6 JF - Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology JO - Hua Xi Kou Qiang Yi Xue Za Zhi VL - 33 IS - 6 N2 - OBJECTIVE: To investigate the effects of small interfering RNA (siRNA) targeting YAP on the proliferation and apoptosis of human periodontal ligament stem cells (hPDLSCs). METHODS: Synthesized sequences of siRNA were transfected into hPDLSCs by Lipofectamine™ 2000. The expression of YAP was identified by using real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analysis. Proliferation activity was detected by using cell counting kit-8 (CCK-8). Changes in the cell cycle and apoptosis rate were detected by using flow cytometry. Results were analyzed by using SPSS 19.0, and P < 0.05 was considered statistically significant. RESULTS: Expression of YAP mRNA and protein were significantly downregulated after 48 h of transfection (P < 0.001). No obvious difference was found in the expression levels of YAP protein between 48 and 72 h, thus indicating that siRNA could inhibit the expression of YAP persistently and effectively. Proliferation activity was inhibited, and apoptosis rate was increased. Cell cycle was changed as the proportion of G₁and S phases increased (P < 0.01) and G₂ phase decreased (P < 0.05). CONCLUSION: Knocking down YAP gene by siRNA could inhibit proliferation activity, induce apoptosis, and change the cell cycle of hPDLSCs. Thus, YAP could regulate the proliferation and apoptosis of hPDLSCs. SN - 1000-1182 UR - https://www.unboundmedicine.com/medline/citation/27051957/[Effects_of_YAP_small_interfering_RNA_on_the_proliferation_and_apoptosis_of_human_periodontal_ligament_stem_cells]_ L2 - http://www.hxkqyxzz.net/fileup/PDF/201506015.pdf DB - PRIME DP - Unbound Medicine ER -