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Serum vitamin D and colonic vitamin D receptor in inflammatory bowel disease.
World J Gastroenterol 2016; 22(13):3581-91WJ

Abstract

AIM

To determine serum vitamin D levels and colonic vitamin D receptor (VDR) expression in inflammatory bowel disease (IBD) and non-IBD patients and correlate these with histopathology.

METHODS

Puerto Rican IBD (n = 10) and non-IBD (n = 10) patients ≥ 21 years old scheduled for colonoscopy were recruited. Each patient completed a questionnaire and provided a serum sample and a colonic biopsy of normal-appearing mucosa. For IBD patients, an additional biopsy was collected from visually diseased mucosa. Serum vitamin D levels were measured by ultra-performance liquid chromatography and mass spectrometry. Hematoxylin and eosin stained tissue sections from colonic biopsies were classified histologically as normal or colitis (active/inactive), and scored for the degree of inflammation present (0-3, inactive/absent to severe). Tissue sections from colonic biopsies were also stained by immunohistochemistry for VDR, for which representative diagnostic areas were photographed and scored for staining intensity using a 4-point scale.

RESULTS

The IBD cohort was significantly younger (40.40 ± 5.27, P < 0.05) than the non-IBD cohort (56.70 ± 1.64) with a higher prevalence of vitamin D deficiency (40% vs 20%, respectively) and insufficiency (70% vs 50%, respectively). Histologic inflammation was significantly higher in visually diseased mucosa from IBD patients (1.95 ± 0.25) than in normal-appearing mucosa from control patients (0.25 ± 0.08, P < 0.01) and from IBD patients (0.65 ± 0.36, P < 0.05) and correlated inversely with VDR expression in visually diseased colonic tissue from IBD patients (r = -0.44, P < 0.05) and from IBD patients with Crohn's disease (r = -0.69, P < 0.05), but not in normal-appearing colonic tissue from control patients or IBD patients. Control and IBD patient serum vitamin D levels correlated positively with VDR expression in normal colon from control and IBD patients (r = 0.38, P < 0.05) and with patient age (r = 0.54, P < 0.01).

CONCLUSION

Levels of serum vitamin D correlate positively with colonic VDR expression in visually normal mucosa whereas inflammation correlates negatively with colonic VDR expression in visually diseased mucosa in Puerto Rican patients.

Authors+Show Affiliations

Yamilka Abreu-Delgado, Esther A Torres, Alexandra González, Priscilla Medero, Division of Gastroenterology, Department of Medicine, University of Puerto Rico Medical Sciences Campus-School of Medicine, San Juan, PR 00935, United States.Yamilka Abreu-Delgado, Esther A Torres, Alexandra González, Priscilla Medero, Division of Gastroenterology, Department of Medicine, University of Puerto Rico Medical Sciences Campus-School of Medicine, San Juan, PR 00935, United States.Yamilka Abreu-Delgado, Esther A Torres, Alexandra González, Priscilla Medero, Division of Gastroenterology, Department of Medicine, University of Puerto Rico Medical Sciences Campus-School of Medicine, San Juan, PR 00935, United States.Yamilka Abreu-Delgado, Esther A Torres, Alexandra González, Priscilla Medero, Division of Gastroenterology, Department of Medicine, University of Puerto Rico Medical Sciences Campus-School of Medicine, San Juan, PR 00935, United States.Yamilka Abreu-Delgado, Esther A Torres, Alexandra González, Priscilla Medero, Division of Gastroenterology, Department of Medicine, University of Puerto Rico Medical Sciences Campus-School of Medicine, San Juan, PR 00935, United States.Yamilka Abreu-Delgado, Esther A Torres, Alexandra González, Priscilla Medero, Division of Gastroenterology, Department of Medicine, University of Puerto Rico Medical Sciences Campus-School of Medicine, San Juan, PR 00935, United States.Yamilka Abreu-Delgado, Esther A Torres, Alexandra González, Priscilla Medero, Division of Gastroenterology, Department of Medicine, University of Puerto Rico Medical Sciences Campus-School of Medicine, San Juan, PR 00935, United States.Yamilka Abreu-Delgado, Esther A Torres, Alexandra González, Priscilla Medero, Division of Gastroenterology, Department of Medicine, University of Puerto Rico Medical Sciences Campus-School of Medicine, San Juan, PR 00935, United States.Yamilka Abreu-Delgado, Esther A Torres, Alexandra González, Priscilla Medero, Division of Gastroenterology, Department of Medicine, University of Puerto Rico Medical Sciences Campus-School of Medicine, San Juan, PR 00935, United States.

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27053850

Citation

Abreu-Delgado, Yamilka, et al. "Serum Vitamin D and Colonic Vitamin D Receptor in Inflammatory Bowel Disease." World Journal of Gastroenterology, vol. 22, no. 13, 2016, pp. 3581-91.
Abreu-Delgado Y, Isidro RA, Torres EA, et al. Serum vitamin D and colonic vitamin D receptor in inflammatory bowel disease. World J Gastroenterol. 2016;22(13):3581-91.
Abreu-Delgado, Y., Isidro, R. A., Torres, E. A., González, A., Cruz, M. L., Isidro, A. A., ... Appleyard, C. B. (2016). Serum vitamin D and colonic vitamin D receptor in inflammatory bowel disease. World Journal of Gastroenterology, 22(13), pp. 3581-91. doi:10.3748/wjg.v22.i13.3581.
Abreu-Delgado Y, et al. Serum Vitamin D and Colonic Vitamin D Receptor in Inflammatory Bowel Disease. World J Gastroenterol. 2016 Apr 7;22(13):3581-91. PubMed PMID: 27053850.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Serum vitamin D and colonic vitamin D receptor in inflammatory bowel disease. AU - Abreu-Delgado,Yamilka, AU - Isidro,Raymond A, AU - Torres,Esther A, AU - González,Alexandra, AU - Cruz,Myrella L, AU - Isidro,Angel A, AU - González-Keelan,Carmen I, AU - Medero,Priscilla, AU - Appleyard,Caroline B, PY - 2015/10/24/received PY - 2015/12/05/revised PY - 2016/01/18/accepted PY - 2016/4/8/entrez PY - 2016/4/8/pubmed PY - 2017/1/18/medline KW - Colitis KW - Inflammation KW - Inflammatory bowel disease KW - Vitamin D KW - Vitamin D receptor SP - 3581 EP - 91 JF - World journal of gastroenterology JO - World J. Gastroenterol. VL - 22 IS - 13 N2 - AIM: To determine serum vitamin D levels and colonic vitamin D receptor (VDR) expression in inflammatory bowel disease (IBD) and non-IBD patients and correlate these with histopathology. METHODS: Puerto Rican IBD (n = 10) and non-IBD (n = 10) patients ≥ 21 years old scheduled for colonoscopy were recruited. Each patient completed a questionnaire and provided a serum sample and a colonic biopsy of normal-appearing mucosa. For IBD patients, an additional biopsy was collected from visually diseased mucosa. Serum vitamin D levels were measured by ultra-performance liquid chromatography and mass spectrometry. Hematoxylin and eosin stained tissue sections from colonic biopsies were classified histologically as normal or colitis (active/inactive), and scored for the degree of inflammation present (0-3, inactive/absent to severe). Tissue sections from colonic biopsies were also stained by immunohistochemistry for VDR, for which representative diagnostic areas were photographed and scored for staining intensity using a 4-point scale. RESULTS: The IBD cohort was significantly younger (40.40 ± 5.27, P < 0.05) than the non-IBD cohort (56.70 ± 1.64) with a higher prevalence of vitamin D deficiency (40% vs 20%, respectively) and insufficiency (70% vs 50%, respectively). Histologic inflammation was significantly higher in visually diseased mucosa from IBD patients (1.95 ± 0.25) than in normal-appearing mucosa from control patients (0.25 ± 0.08, P < 0.01) and from IBD patients (0.65 ± 0.36, P < 0.05) and correlated inversely with VDR expression in visually diseased colonic tissue from IBD patients (r = -0.44, P < 0.05) and from IBD patients with Crohn's disease (r = -0.69, P < 0.05), but not in normal-appearing colonic tissue from control patients or IBD patients. Control and IBD patient serum vitamin D levels correlated positively with VDR expression in normal colon from control and IBD patients (r = 0.38, P < 0.05) and with patient age (r = 0.54, P < 0.01). CONCLUSION: Levels of serum vitamin D correlate positively with colonic VDR expression in visually normal mucosa whereas inflammation correlates negatively with colonic VDR expression in visually diseased mucosa in Puerto Rican patients. SN - 2219-2840 UR - https://www.unboundmedicine.com/medline/citation/27053850/Serum_vitamin_D_and_colonic_vitamin_D_receptor_in_inflammatory_bowel_disease_ L2 - http://www.wjgnet.com/1007-9327/full/v22/i13/3581.htm DB - PRIME DP - Unbound Medicine ER -