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Naloxone pretreatment prevents kidney injury after liver ischemia reperfusion injury.
Int Urol Nephrol. 2016 Jul; 48(7):1113-20.IU

Abstract

PURPOSE

The aim of this study was to assess the effects of naloxone, an opioid receptor antagonist, on the renal injury as a remote organ after hepatic ischemia reperfusion (IR) in rats.

MATERIALS AND METHODS

Forty male Wistar rats were randomly allocated into four groups as follows: sham, sham + naloxone, IR and IR + naloxone. In anesthetized rats, hepatic ischemia was applied for 30 min in IR and IR + naloxone groups. Sham + naloxone and IR + naloxone groups were given naloxone (3.0 mg/kg, iv) 30 min before ischemia. After 24 h, blood and tissue samples were obtained for histopathological, tissue malondialdehyde (MDA) and biochemical analyses.

RESULTS

Histopathological study of liver in IR group showed enlarged sinusoids, sinusoidal congestion, cellular degenerative changes and necrosis. The kidney of the rats with hepatic IR showed pathological changes in tubular cell swelling, tubular dilatation, moderate to severe necrosis, glomerular fibrosis and hemorrhage. Histological examination confirmed the extent of hepatic and renal changes in IR group was higher (P < 0.05) than in other groups. Rats that underwent hepatic IR exhibited significant increase in serum concentrations of urea and creatinine levels (P < 0.05). The serum alanine aminotransferase and aminotransferase values were significantly higher in IR group compared to the other groups (P < 0.05). Liver IR produced a significant increase in hepatic and renal tissue MDA levels, while pretreatment with naloxone was associated with a significantly lower MDA levels (P < 0.05).

CONCLUSION

The results of this study showed that naloxone pretreatment protected the renal injury from hepatic IR.

Authors+Show Affiliations

Young Researchers and Elites Club, Science and Research Branch, Islamic Azad University, Tehran, Iran. dr_ashrafzadeh@yahoo.com.Department of Cardiovascular Surgery, Isfahan University of Medical Sciences, Isfahan, Iran.Department of Clinical Science, Science and Research Branch, Islamic Azad University, Tehran, Iran.Graduate Student of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27055556

Citation

Takhtfooladi, Mohammad Ashrafzadeh, et al. "Naloxone Pretreatment Prevents Kidney Injury After Liver Ischemia Reperfusion Injury." International Urology and Nephrology, vol. 48, no. 7, 2016, pp. 1113-20.
Takhtfooladi MA, Shahzamani M, Asghari A, et al. Naloxone pretreatment prevents kidney injury after liver ischemia reperfusion injury. Int Urol Nephrol. 2016;48(7):1113-20.
Takhtfooladi, M. A., Shahzamani, M., Asghari, A., & Fakouri, A. (2016). Naloxone pretreatment prevents kidney injury after liver ischemia reperfusion injury. International Urology and Nephrology, 48(7), 1113-20. https://doi.org/10.1007/s11255-016-1280-5
Takhtfooladi MA, et al. Naloxone Pretreatment Prevents Kidney Injury After Liver Ischemia Reperfusion Injury. Int Urol Nephrol. 2016;48(7):1113-20. PubMed PMID: 27055556.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Naloxone pretreatment prevents kidney injury after liver ischemia reperfusion injury. AU - Takhtfooladi,Mohammad Ashrafzadeh, AU - Shahzamani,Mehran, AU - Asghari,Ahmad, AU - Fakouri,Aris, Y1 - 2016/04/07/ PY - 2015/07/02/received PY - 2016/03/26/accepted PY - 2016/4/9/entrez PY - 2016/4/9/pubmed PY - 2017/7/20/medline KW - Ischemia reperfusion KW - Kidney KW - Liver KW - Naloxone KW - Remote organ SP - 1113 EP - 20 JF - International urology and nephrology JO - Int Urol Nephrol VL - 48 IS - 7 N2 - PURPOSE: The aim of this study was to assess the effects of naloxone, an opioid receptor antagonist, on the renal injury as a remote organ after hepatic ischemia reperfusion (IR) in rats. MATERIALS AND METHODS: Forty male Wistar rats were randomly allocated into four groups as follows: sham, sham + naloxone, IR and IR + naloxone. In anesthetized rats, hepatic ischemia was applied for 30 min in IR and IR + naloxone groups. Sham + naloxone and IR + naloxone groups were given naloxone (3.0 mg/kg, iv) 30 min before ischemia. After 24 h, blood and tissue samples were obtained for histopathological, tissue malondialdehyde (MDA) and biochemical analyses. RESULTS: Histopathological study of liver in IR group showed enlarged sinusoids, sinusoidal congestion, cellular degenerative changes and necrosis. The kidney of the rats with hepatic IR showed pathological changes in tubular cell swelling, tubular dilatation, moderate to severe necrosis, glomerular fibrosis and hemorrhage. Histological examination confirmed the extent of hepatic and renal changes in IR group was higher (P < 0.05) than in other groups. Rats that underwent hepatic IR exhibited significant increase in serum concentrations of urea and creatinine levels (P < 0.05). The serum alanine aminotransferase and aminotransferase values were significantly higher in IR group compared to the other groups (P < 0.05). Liver IR produced a significant increase in hepatic and renal tissue MDA levels, while pretreatment with naloxone was associated with a significantly lower MDA levels (P < 0.05). CONCLUSION: The results of this study showed that naloxone pretreatment protected the renal injury from hepatic IR. SN - 1573-2584 UR - https://www.unboundmedicine.com/medline/citation/27055556/Naloxone_pretreatment_prevents_kidney_injury_after_liver_ischemia_reperfusion_injury_ L2 - https://doi.org/10.1007/s11255-016-1280-5 DB - PRIME DP - Unbound Medicine ER -