Tags

Type your tag names separated by a space and hit enter

Ameliorative effect of berberine against gentamicin-induced nephrotoxicity in rats via attenuation of oxidative stress, inflammation, apoptosis and mitochondrial dysfunction.
Ren Fail. 2016 Jul; 38(6):996-1006.RF

Abstract

BACKGROUND

Gentamicin (GM) is the commonly used antibiotics against Gram-negative infection, but the nephrotoxic potential of drug limit its clinical interest. The aim of this study was to investigate the protective effect of berberine (BER) against GM-induced nephrotoxicity and possible underlying mechanisms.

MATERIAL AND METHODS

The rats were divided into various group, namely normal, GM-control, GM + BER (10, 20, and 40 mg/kg). Nephrotoxicity was induced by intraperitoneal administration of GM (120 mg/kg) for 7 consecutive days. BER (10, 20, and 40 mg/kg; p.o.) was also administered for the 7 days. Various biochemical, molecular, and histological parameters were assessed in serum and kidney.

RESULTS

GM-administration significantly increased (p < 0.001) the serum creatinine and blood urea nitrogen (BUN) as well as renal malonaldehyde (MDA), nitric oxide (NO) along with Kidney Injury Molecule-1 (KIM-1), Neutrophil gelatinase-associated lipocalin (NGAL), and nuclear factor-kappa B (NF-KB) renal mRNA expressions. In addition, GM also significantly decreased (p < 0.001) the renal superoxide dismutase (SOD), reduced glutathione (GSH), B-cell lymphoma 2 (Bcl-2) mRNA expression, and mitochondrial enzymes (NADH dehydrogenase and cytochrome c oxidase) activities. Rats treated with BER (20 and 40 mg/kg; p.o.) significantly and dose-dependently (p < 0.05 and p < 0.01) restore the altered levels of antioxidant, inflammatory, apoptosis, AKI markers as well as depleted mitochondrial enzymes. Histopathological abbreviations were also ameliorated by BER administration.

CONCLUSION

Berberine exerts renoprotective effects through its anti-oxidant, anti-inflammatory, and anti-apoptotic properties.[Formula: see text].

Authors+Show Affiliations

a Department of Pharmacology , Poona College of Pharmacy, Bharati Vidyapeeth Deemed University , Pune , Maharashtra , India.a Department of Pharmacology , Poona College of Pharmacy, Bharati Vidyapeeth Deemed University , Pune , Maharashtra , India.a Department of Pharmacology , Poona College of Pharmacy, Bharati Vidyapeeth Deemed University , Pune , Maharashtra , India.a Department of Pharmacology , Poona College of Pharmacy, Bharati Vidyapeeth Deemed University , Pune , Maharashtra , India.a Department of Pharmacology , Poona College of Pharmacy, Bharati Vidyapeeth Deemed University , Pune , Maharashtra , India.a Department of Pharmacology , Poona College of Pharmacy, Bharati Vidyapeeth Deemed University , Pune , Maharashtra , India.a Department of Pharmacology , Poona College of Pharmacy, Bharati Vidyapeeth Deemed University , Pune , Maharashtra , India.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27056079

Citation

Adil, Mohammad, et al. "Ameliorative Effect of Berberine Against Gentamicin-induced Nephrotoxicity in Rats Via Attenuation of Oxidative Stress, Inflammation, Apoptosis and Mitochondrial Dysfunction." Renal Failure, vol. 38, no. 6, 2016, pp. 996-1006.
Adil M, Kandhare AD, Dalvi G, et al. Ameliorative effect of berberine against gentamicin-induced nephrotoxicity in rats via attenuation of oxidative stress, inflammation, apoptosis and mitochondrial dysfunction. Ren Fail. 2016;38(6):996-1006.
Adil, M., Kandhare, A. D., Dalvi, G., Ghosh, P., Venkata, S., Raygude, K. S., & Bodhankar, S. L. (2016). Ameliorative effect of berberine against gentamicin-induced nephrotoxicity in rats via attenuation of oxidative stress, inflammation, apoptosis and mitochondrial dysfunction. Renal Failure, 38(6), 996-1006. https://doi.org/10.3109/0886022X.2016.1165120
Adil M, et al. Ameliorative Effect of Berberine Against Gentamicin-induced Nephrotoxicity in Rats Via Attenuation of Oxidative Stress, Inflammation, Apoptosis and Mitochondrial Dysfunction. Ren Fail. 2016;38(6):996-1006. PubMed PMID: 27056079.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ameliorative effect of berberine against gentamicin-induced nephrotoxicity in rats via attenuation of oxidative stress, inflammation, apoptosis and mitochondrial dysfunction. AU - Adil,Mohammad, AU - Kandhare,Amit D, AU - Dalvi,Gautami, AU - Ghosh,Pinaki, AU - Venkata,Shivakumar, AU - Raygude,Kiran S, AU - Bodhankar,Subhash L, Y1 - 2016/04/08/ PY - 2016/4/9/entrez PY - 2016/4/9/pubmed PY - 2017/4/11/medline KW - Bcl-2 KW - KIM-1 KW - NF-KB KW - NGAL KW - berberine KW - gentamicin KW - nephrotoxicity SP - 996 EP - 1006 JF - Renal failure JO - Ren Fail VL - 38 IS - 6 N2 - BACKGROUND: Gentamicin (GM) is the commonly used antibiotics against Gram-negative infection, but the nephrotoxic potential of drug limit its clinical interest. The aim of this study was to investigate the protective effect of berberine (BER) against GM-induced nephrotoxicity and possible underlying mechanisms. MATERIAL AND METHODS: The rats were divided into various group, namely normal, GM-control, GM + BER (10, 20, and 40 mg/kg). Nephrotoxicity was induced by intraperitoneal administration of GM (120 mg/kg) for 7 consecutive days. BER (10, 20, and 40 mg/kg; p.o.) was also administered for the 7 days. Various biochemical, molecular, and histological parameters were assessed in serum and kidney. RESULTS: GM-administration significantly increased (p < 0.001) the serum creatinine and blood urea nitrogen (BUN) as well as renal malonaldehyde (MDA), nitric oxide (NO) along with Kidney Injury Molecule-1 (KIM-1), Neutrophil gelatinase-associated lipocalin (NGAL), and nuclear factor-kappa B (NF-KB) renal mRNA expressions. In addition, GM also significantly decreased (p < 0.001) the renal superoxide dismutase (SOD), reduced glutathione (GSH), B-cell lymphoma 2 (Bcl-2) mRNA expression, and mitochondrial enzymes (NADH dehydrogenase and cytochrome c oxidase) activities. Rats treated with BER (20 and 40 mg/kg; p.o.) significantly and dose-dependently (p < 0.05 and p < 0.01) restore the altered levels of antioxidant, inflammatory, apoptosis, AKI markers as well as depleted mitochondrial enzymes. Histopathological abbreviations were also ameliorated by BER administration. CONCLUSION: Berberine exerts renoprotective effects through its anti-oxidant, anti-inflammatory, and anti-apoptotic properties.[Formula: see text]. SN - 1525-6049 UR - https://www.unboundmedicine.com/medline/citation/27056079/Ameliorative_effect_of_berberine_against_gentamicin_induced_nephrotoxicity_in_rats_via_attenuation_of_oxidative_stress_inflammation_apoptosis_and_mitochondrial_dysfunction_ L2 - https://www.tandfonline.com/doi/full/10.3109/0886022X.2016.1165120 DB - PRIME DP - Unbound Medicine ER -