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The H2S Donor NaHS Changes the Expression Pattern of H2S-Producing Enzymes after Myocardial Infarction.
Oxid Med Cell Longev. 2016; 2016:6492469.OM

Abstract

Aims. To examine the expression patterns of hydrogen sulphide- (H2S-) producing enzymes in ischaemic heart tissue and plasma levels of H2S after 2 weeks of NaHS treatment after myocardial infarction (MI) and to clarify the role of endogenous H2S in the MI process.

Results.

After MI surgery, 2 weeks of treatment with the H2S donor NaHS alleviated ischaemic injury. Meanwhile, in ischemia myocardium, three H2S-producing enzymes, cystathionine γ-lyase (CSE), cystathionine-β-synthase (CBS), and 3-mercaptopyruvate sulfurtransferase (3-MST) significantly increased. Plasma H2S levels were also elevated. In vitro, NaHS treatment protected cardiomyocytes from hypoxic injury and raised CBS levels in a concentration-dependent manner. Different from in vivo results, however, CSE or 3-MST expression did not change. NaHS treatment increased the activity of CSE/CBS but not of 3-MST. When CSE was either knocked down (in vitro) or knocked out (in vivo), H2S levels significantly decreased, which subsequently exacerbated the ischaemic injury. Meanwhile, the expressions of CBS and 3-MST increased due to compensation.

Conclusions.

Exogenous H2S treatment changed the expressions of three H2S-producing enzymes and H2S levels after MI, suggesting a new and indirect regulatory mechanism for H2S production and its contribution to cardiac protection. Endogenous H2S plays an important role in protecting ischaemic tissue after MI.

Authors+Show Affiliations

Shanghai Key Laboratory of Bioactive Small Molecules, Research Center on Aging and Medicine, Department of Physiology and Pathophysiology, Shanghai Medical College, Fudan University, Shanghai 200032, China; Center for Developmental Cardiology, Institute for Translational Medicine, College of Medicine, Qingdao University, Qingdao 266021, China.Shanghai Key Laboratory of Bioactive Small Molecules, Research Center on Aging and Medicine, Department of Physiology and Pathophysiology, Shanghai Medical College, Fudan University, Shanghai 200032, China.Shanghai Key Laboratory of Bioactive Small Molecules, Research Center on Aging and Medicine, Department of Physiology and Pathophysiology, Shanghai Medical College, Fudan University, Shanghai 200032, China; Department of Physiology, Hebei Medical University, Hebei 050017, China.Shanghai Key Laboratory of Bioactive Small Molecules, Research Center on Aging and Medicine, Department of Physiology and Pathophysiology, Shanghai Medical College, Fudan University, Shanghai 200032, China.Shanghai Key Laboratory of Bioactive Small Molecules, Research Center on Aging and Medicine, Department of Physiology and Pathophysiology, Shanghai Medical College, Fudan University, Shanghai 200032, China.Department of Pharmacology, School of Pharmacy, Fudan University, Shanghai 201203, China.Shanghai Key Laboratory of Bioactive Small Molecules, Research Center on Aging and Medicine, Department of Physiology and Pathophysiology, Shanghai Medical College, Fudan University, Shanghai 200032, China.Shanghai Key Laboratory of Bioactive Small Molecules, Research Center on Aging and Medicine, Department of Physiology and Pathophysiology, Shanghai Medical College, Fudan University, Shanghai 200032, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27057284

Citation

Li, Na, et al. "The H2S Donor NaHS Changes the Expression Pattern of H2S-Producing Enzymes After Myocardial Infarction." Oxidative Medicine and Cellular Longevity, vol. 2016, 2016, p. 6492469.
Li N, Wang MJ, Jin S, et al. The H2S Donor NaHS Changes the Expression Pattern of H2S-Producing Enzymes after Myocardial Infarction. Oxid Med Cell Longev. 2016;2016:6492469.
Li, N., Wang, M. J., Jin, S., Bai, Y. D., Hou, C. L., Ma, F. F., Li, X. H., & Zhu, Y. C. (2016). The H2S Donor NaHS Changes the Expression Pattern of H2S-Producing Enzymes after Myocardial Infarction. Oxidative Medicine and Cellular Longevity, 2016, 6492469. https://doi.org/10.1155/2016/6492469
Li N, et al. The H2S Donor NaHS Changes the Expression Pattern of H2S-Producing Enzymes After Myocardial Infarction. Oxid Med Cell Longev. 2016;2016:6492469. PubMed PMID: 27057284.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The H2S Donor NaHS Changes the Expression Pattern of H2S-Producing Enzymes after Myocardial Infarction. AU - Li,Na, AU - Wang,Ming-Jie, AU - Jin,Sheng, AU - Bai,Ya-Dan, AU - Hou,Cui-Lan, AU - Ma,Fen-Fen, AU - Li,Xing-Hui, AU - Zhu,Yi-Chun, Y1 - 2016/01/05/ PY - 2015/09/08/received PY - 2015/11/09/revised PY - 2015/11/30/accepted PY - 2016/4/9/entrez PY - 2016/4/9/pubmed PY - 2016/12/27/medline SP - 6492469 EP - 6492469 JF - Oxidative medicine and cellular longevity JO - Oxid Med Cell Longev VL - 2016 N2 - Aims. To examine the expression patterns of hydrogen sulphide- (H2S-) producing enzymes in ischaemic heart tissue and plasma levels of H2S after 2 weeks of NaHS treatment after myocardial infarction (MI) and to clarify the role of endogenous H2S in the MI process. Results. After MI surgery, 2 weeks of treatment with the H2S donor NaHS alleviated ischaemic injury. Meanwhile, in ischemia myocardium, three H2S-producing enzymes, cystathionine γ-lyase (CSE), cystathionine-β-synthase (CBS), and 3-mercaptopyruvate sulfurtransferase (3-MST) significantly increased. Plasma H2S levels were also elevated. In vitro, NaHS treatment protected cardiomyocytes from hypoxic injury and raised CBS levels in a concentration-dependent manner. Different from in vivo results, however, CSE or 3-MST expression did not change. NaHS treatment increased the activity of CSE/CBS but not of 3-MST. When CSE was either knocked down (in vitro) or knocked out (in vivo), H2S levels significantly decreased, which subsequently exacerbated the ischaemic injury. Meanwhile, the expressions of CBS and 3-MST increased due to compensation. Conclusions. Exogenous H2S treatment changed the expressions of three H2S-producing enzymes and H2S levels after MI, suggesting a new and indirect regulatory mechanism for H2S production and its contribution to cardiac protection. Endogenous H2S plays an important role in protecting ischaemic tissue after MI. SN - 1942-0994 UR - https://www.unboundmedicine.com/medline/citation/27057284/The_H2S_Donor_NaHS_Changes_the_Expression_Pattern_of_H2S_Producing_Enzymes_after_Myocardial_Infarction_ L2 - https://doi.org/10.1155/2016/6492469 DB - PRIME DP - Unbound Medicine ER -