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Expression of Castor LPAT2 Enhances Ricinoleic Acid Content at the sn-2 Position of Triacylglycerols in Lesquerella Seed.
Int J Mol Sci. 2016 Apr 06; 17(4):507.IJ

Abstract

Lesquerella is a potential industrial oilseed crop that makes hydroxy fatty acid (HFA). Unlike castor its seeds are not poisonous but accumulate lesquerolic acid mostly at the sn-1 and sn-3 positions of triacylglycerol (TAG), whereas castor contains ricinoleic acid (18:1OH) at all three positions. To investigate whether lesquerella can be engineered to accumulate HFAs in the sn-2 position, multiple transgenic lines were made that express castor lysophosphatidic acid acyltransferase 2 (RcLPAT2) in the seed. RcLPAT2 increased 18:1OH at the sn-2 position of TAGs from 2% to 14%-17%, which resulted in an increase of tri-HFA-TAGs from 5% to 13%-14%. Our result is the first example of using a LPAT to increase ricinoleic acid at the sn-2 position of seed TAG. This work provides insights to the mechanism of HFA-containing TAG assembly in lesquerella and directs future research to optimize this plant for HFA production.

Authors+Show Affiliations

Western Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, 800 Buchanan St., Albany, CA 94710, USA. grace.chen@ars.usda.gov.Institute of Biological Chemistry, Washington State University, Pullman, Washington, DC 99164, USA. harrie.van-erp@rothamsted.ac.uk. Department of Plant Biology and Crop Science, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK. harrie.van-erp@rothamsted.ac.uk.Department of Plant Biology and Crop Science, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK. jmartinm83@gmail.com.Western Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, 800 Buchanan St., Albany, CA 94710, USA. kumiko.johnson@ars.usda.gov.Western Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, 800 Buchanan St., Albany, CA 94710, USA. evadesalta@gmail.com.Institute of Biological Chemistry, Washington State University, Pullman, Washington, DC 99164, USA. jab@wsu.edu.Department of Plant Biology and Crop Science, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK. peter.eastmond@rothamsted.ac.uk.Western Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, 800 Buchanan St., Albany, CA 94710, USA. jiann.lin@ars.usda.gov.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

27058535

Citation

Chen, Grace Q., et al. "Expression of Castor LPAT2 Enhances Ricinoleic Acid Content at the Sn-2 Position of Triacylglycerols in Lesquerella Seed." International Journal of Molecular Sciences, vol. 17, no. 4, 2016, p. 507.
Chen GQ, van Erp H, Martin-Moreno J, et al. Expression of Castor LPAT2 Enhances Ricinoleic Acid Content at the sn-2 Position of Triacylglycerols in Lesquerella Seed. Int J Mol Sci. 2016;17(4):507.
Chen, G. Q., van Erp, H., Martin-Moreno, J., Johnson, K., Morales, E., Browse, J., Eastmond, P. J., & Lin, J. T. (2016). Expression of Castor LPAT2 Enhances Ricinoleic Acid Content at the sn-2 Position of Triacylglycerols in Lesquerella Seed. International Journal of Molecular Sciences, 17(4), 507. https://doi.org/10.3390/ijms17040507
Chen GQ, et al. Expression of Castor LPAT2 Enhances Ricinoleic Acid Content at the Sn-2 Position of Triacylglycerols in Lesquerella Seed. Int J Mol Sci. 2016 Apr 6;17(4):507. PubMed PMID: 27058535.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of Castor LPAT2 Enhances Ricinoleic Acid Content at the sn-2 Position of Triacylglycerols in Lesquerella Seed. AU - Chen,Grace Q, AU - van Erp,Harrie, AU - Martin-Moreno,Jose, AU - Johnson,Kumiko, AU - Morales,Eva, AU - Browse,John, AU - Eastmond,Peter J, AU - Lin,Jiann-Tsyh, Y1 - 2016/04/06/ PY - 2016/03/04/received PY - 2016/03/25/revised PY - 2016/03/30/accepted PY - 2016/4/9/entrez PY - 2016/4/9/pubmed PY - 2016/12/16/medline KW - Physaria fendleri KW - hydroxy fatty acid KW - lesquerella KW - lysophosphatidic acid acyltransferase KW - metabolic engineering KW - seed triacylglycerol SP - 507 EP - 507 JF - International journal of molecular sciences JO - Int J Mol Sci VL - 17 IS - 4 N2 - Lesquerella is a potential industrial oilseed crop that makes hydroxy fatty acid (HFA). Unlike castor its seeds are not poisonous but accumulate lesquerolic acid mostly at the sn-1 and sn-3 positions of triacylglycerol (TAG), whereas castor contains ricinoleic acid (18:1OH) at all three positions. To investigate whether lesquerella can be engineered to accumulate HFAs in the sn-2 position, multiple transgenic lines were made that express castor lysophosphatidic acid acyltransferase 2 (RcLPAT2) in the seed. RcLPAT2 increased 18:1OH at the sn-2 position of TAGs from 2% to 14%-17%, which resulted in an increase of tri-HFA-TAGs from 5% to 13%-14%. Our result is the first example of using a LPAT to increase ricinoleic acid at the sn-2 position of seed TAG. This work provides insights to the mechanism of HFA-containing TAG assembly in lesquerella and directs future research to optimize this plant for HFA production. SN - 1422-0067 UR - https://www.unboundmedicine.com/medline/citation/27058535/Expression_of_Castor_LPAT2_Enhances_Ricinoleic_Acid_Content_at_the_sn_2_Position_of_Triacylglycerols_in_Lesquerella_Seed_ L2 - https://www.mdpi.com/resolver?pii=ijms17040507 DB - PRIME DP - Unbound Medicine ER -