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Investigations on the role of leukotrienes in remote hind limb preconditioning-induced cardioprotection in rats.
Life Sci. 2016 May 01; 152:238-43.LS

Abstract

The cardioprotective effects of remote hind limb preconditioning (RIPC) are well established, but its mechanisms still remain to be explored. Therefore, the present study was aimed to explore the possible involvement of 5-lipoxygenase-derived leukotrienes in RIPC. The hind limb was tied by a pressure cuff and was subjected to four episodes of inflation and deflation (5min each) to induce remote hind-limb preconditioning. Thereafter, the hearts were isolated and were subjected to global ischemia (30min) followed by reperfusion (120min) on the Langendorff apparatus. The extent of myocardial injury was assessed by measuring lactate dehydrogenase (LDH) and creatine kinase (CK) levels in the coronary effluent; the infarct size using TTC staining, and the hemodynamic parameters including LVDP, dp/dtmax and dp/dtmin. RIPC significantly decreased ischemia and reperfusion-induced increase in LDH, CK release, infarct size and improved LVDP, dp/dtmax and dp/dtmin. Administration of montelukast, leukotriene receptor antagonist (10 and 20mg/kg) and zileuton, 5-lipoxygenase inhibitor, (2.5 and 5mg/kg) abolished RIPC-induced cardioprotection. It may be concluded that hind limb ischemia stimulates 5-lipoxygenase to release leukotrienes which may elicit cardioprotection by humoral or neurogenic pathway.

Authors+Show Affiliations

Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala 147002, India.Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala 147002, India.Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala 147002, India.Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala 147002, India. Electronic address: amteshwarjaggi@yahoo.co.in.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27058978

Citation

Singh, Baljeet, et al. "Investigations On the Role of Leukotrienes in Remote Hind Limb Preconditioning-induced Cardioprotection in Rats." Life Sciences, vol. 152, 2016, pp. 238-43.
Singh B, Randhawa PK, Singh N, et al. Investigations on the role of leukotrienes in remote hind limb preconditioning-induced cardioprotection in rats. Life Sci. 2016;152:238-43.
Singh, B., Randhawa, P. K., Singh, N., & Jaggi, A. S. (2016). Investigations on the role of leukotrienes in remote hind limb preconditioning-induced cardioprotection in rats. Life Sciences, 152, 238-43. https://doi.org/10.1016/j.lfs.2016.04.005
Singh B, et al. Investigations On the Role of Leukotrienes in Remote Hind Limb Preconditioning-induced Cardioprotection in Rats. Life Sci. 2016 May 1;152:238-43. PubMed PMID: 27058978.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Investigations on the role of leukotrienes in remote hind limb preconditioning-induced cardioprotection in rats. AU - Singh,Baljeet, AU - Randhawa,Puneet Kaur, AU - Singh,Nirmal, AU - Jaggi,Amteshwar Singh, Y1 - 2016/04/06/ PY - 2015/07/29/received PY - 2016/03/21/revised PY - 2016/04/03/accepted PY - 2016/4/10/entrez PY - 2016/4/10/pubmed PY - 2017/2/9/medline KW - Cardioprotection KW - Leukotrienes KW - Montelukast KW - Remote hind limb preconditioning KW - Zileuton SP - 238 EP - 43 JF - Life sciences JO - Life Sci VL - 152 N2 - The cardioprotective effects of remote hind limb preconditioning (RIPC) are well established, but its mechanisms still remain to be explored. Therefore, the present study was aimed to explore the possible involvement of 5-lipoxygenase-derived leukotrienes in RIPC. The hind limb was tied by a pressure cuff and was subjected to four episodes of inflation and deflation (5min each) to induce remote hind-limb preconditioning. Thereafter, the hearts were isolated and were subjected to global ischemia (30min) followed by reperfusion (120min) on the Langendorff apparatus. The extent of myocardial injury was assessed by measuring lactate dehydrogenase (LDH) and creatine kinase (CK) levels in the coronary effluent; the infarct size using TTC staining, and the hemodynamic parameters including LVDP, dp/dtmax and dp/dtmin. RIPC significantly decreased ischemia and reperfusion-induced increase in LDH, CK release, infarct size and improved LVDP, dp/dtmax and dp/dtmin. Administration of montelukast, leukotriene receptor antagonist (10 and 20mg/kg) and zileuton, 5-lipoxygenase inhibitor, (2.5 and 5mg/kg) abolished RIPC-induced cardioprotection. It may be concluded that hind limb ischemia stimulates 5-lipoxygenase to release leukotrienes which may elicit cardioprotection by humoral or neurogenic pathway. SN - 1879-0631 UR - https://www.unboundmedicine.com/medline/citation/27058978/Investigations_on_the_role_of_leukotrienes_in_remote_hind_limb_preconditioning_induced_cardioprotection_in_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0024-3205(16)30225-9 DB - PRIME DP - Unbound Medicine ER -