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Densitometry of Choroidal Vessels in Eyes With and Without Central Serous Chorioretinopathy by Wide-Field Indocyanine Green Angiography.
Am J Ophthalmol 2016; 166:103-111AJ

Abstract

PURPOSE

To develop a new method to quantify the choroidal vessel density by binarizing ultra-wide-field indocyanine green angiography (ICGA) images and determine whether values are altered in diseased eyes.

DESIGN

Reliability and validity analysis.

METHODS

Ultra-wide-field fluorescein angiography (FA) and ICGA images were obtained using an ultra-wide-field imaging device (Optos California ultra-wide-field imaging device; Dunfermline, Scotland, UK) in 11 eyes of 11 patients without chorioretinal diseases. The angiographic signals of the choroidal vessels were determined by subtracting those of the retinal vasculature and optic disc on the FA images from the ICGA images, binarized by Niblack's method, and the choroidal vessel density calculated. Reproducibility of the method was assessed by calculating the coefficient of variance, coefficient of repeatability, and intraclass correlation coefficient. The relationships between age, spherical equivalent refractive error (SERE), and intraocular pressure and the vasculature density were assessed. To investigate possible impacts of chorioretinal diseases on the vasculature density, 10 eyes of 7 patients with central serous chorioretinopathy (CSC) were compared with the 11 control eyes.

RESULTS

Choroidal vessels were contrasted by binarizing ICGA images. The method to quantify the choroidal vessel density showed high reproducibility. The SERE was correlated significantly (r = 0.573, P < .05) with the vasculature density. In the 11 control eyes, the vasculature density was 34.26% ± 0.77% in the entire area, 31.37% ± 0.97% in the superior portion, 36.98% ± 0.88% in the inferior portion, 37.01% ± 1.44% in the posterior portion, and 34.17% ± 0.77% in the peripheral portion. In eyes with CSC, the density was significantly (P < .05) higher: 36.46% ± 0.49%, 34.02% ± 0.97%, 38.65% ± 0.27%, 41.04% ± 0.82%, and 36.36% ± 0.51%, respectively.

CONCLUSIONS

Binarization of ultra-wide-field ICGA images enabled quantification of the choroidal vessel density, which was altered in eyes with CSC. This method of measuring the choroidal vessel density may provide new insights into diagnosing and treating chorioretinal diseases.

Authors+Show Affiliations

Department of Ophthalmology and Visual Science, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan.Department of Ophthalmology and Visual Science, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan. Electronic address: yasukawa@med.nagoya-cu.ac.jp.Department of Ophthalmology and Visual Science, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan.Department of Ophthalmology and Visual Science, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan.Department of Ophthalmology and Visual Science, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan.

Pub Type(s)

Journal Article
Validation Studies

Language

eng

PubMed ID

27059632

Citation

Hirahara, Shuichiro, et al. "Densitometry of Choroidal Vessels in Eyes With and Without Central Serous Chorioretinopathy By Wide-Field Indocyanine Green Angiography." American Journal of Ophthalmology, vol. 166, 2016, pp. 103-111.
Hirahara S, Yasukawa T, Kominami A, et al. Densitometry of Choroidal Vessels in Eyes With and Without Central Serous Chorioretinopathy by Wide-Field Indocyanine Green Angiography. Am J Ophthalmol. 2016;166:103-111.
Hirahara, S., Yasukawa, T., Kominami, A., Nozaki, M., & Ogura, Y. (2016). Densitometry of Choroidal Vessels in Eyes With and Without Central Serous Chorioretinopathy by Wide-Field Indocyanine Green Angiography. American Journal of Ophthalmology, 166, pp. 103-111. doi:10.1016/j.ajo.2016.03.040.
Hirahara S, et al. Densitometry of Choroidal Vessels in Eyes With and Without Central Serous Chorioretinopathy By Wide-Field Indocyanine Green Angiography. Am J Ophthalmol. 2016;166:103-111. PubMed PMID: 27059632.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Densitometry of Choroidal Vessels in Eyes With and Without Central Serous Chorioretinopathy by Wide-Field Indocyanine Green Angiography. AU - Hirahara,Shuichiro, AU - Yasukawa,Tsutomu, AU - Kominami,Aoi, AU - Nozaki,Miho, AU - Ogura,Yuichiro, Y1 - 2016/04/06/ PY - 2016/01/08/received PY - 2016/03/24/revised PY - 2016/03/28/accepted PY - 2016/4/10/entrez PY - 2016/4/10/pubmed PY - 2017/5/4/medline SP - 103 EP - 111 JF - American journal of ophthalmology JO - Am. J. Ophthalmol. VL - 166 N2 - PURPOSE: To develop a new method to quantify the choroidal vessel density by binarizing ultra-wide-field indocyanine green angiography (ICGA) images and determine whether values are altered in diseased eyes. DESIGN: Reliability and validity analysis. METHODS: Ultra-wide-field fluorescein angiography (FA) and ICGA images were obtained using an ultra-wide-field imaging device (Optos California ultra-wide-field imaging device; Dunfermline, Scotland, UK) in 11 eyes of 11 patients without chorioretinal diseases. The angiographic signals of the choroidal vessels were determined by subtracting those of the retinal vasculature and optic disc on the FA images from the ICGA images, binarized by Niblack's method, and the choroidal vessel density calculated. Reproducibility of the method was assessed by calculating the coefficient of variance, coefficient of repeatability, and intraclass correlation coefficient. The relationships between age, spherical equivalent refractive error (SERE), and intraocular pressure and the vasculature density were assessed. To investigate possible impacts of chorioretinal diseases on the vasculature density, 10 eyes of 7 patients with central serous chorioretinopathy (CSC) were compared with the 11 control eyes. RESULTS: Choroidal vessels were contrasted by binarizing ICGA images. The method to quantify the choroidal vessel density showed high reproducibility. The SERE was correlated significantly (r = 0.573, P < .05) with the vasculature density. In the 11 control eyes, the vasculature density was 34.26% ± 0.77% in the entire area, 31.37% ± 0.97% in the superior portion, 36.98% ± 0.88% in the inferior portion, 37.01% ± 1.44% in the posterior portion, and 34.17% ± 0.77% in the peripheral portion. In eyes with CSC, the density was significantly (P < .05) higher: 36.46% ± 0.49%, 34.02% ± 0.97%, 38.65% ± 0.27%, 41.04% ± 0.82%, and 36.36% ± 0.51%, respectively. CONCLUSIONS: Binarization of ultra-wide-field ICGA images enabled quantification of the choroidal vessel density, which was altered in eyes with CSC. This method of measuring the choroidal vessel density may provide new insights into diagnosing and treating chorioretinal diseases. SN - 1879-1891 UR - https://www.unboundmedicine.com/medline/citation/27059632/Densitometry_of_Choroidal_Vessels_in_Eyes_With_and_Without_Central_Serous_Chorioretinopathy_by_Wide_Field_Indocyanine_Green_Angiography_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0002-9394(16)30144-1 DB - PRIME DP - Unbound Medicine ER -