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Rich haplotypes of Viviparous-1 in Triticum aestivum subsp. spelta with different abscisic acid sensitivities.
J Sci Food Agric. 2017 Jan; 97(2):497-504.JS

Abstract

BACKGROUND

Viviparous-1 (Vp-1) is a major gene affecting pre-harvest sprouting (PHS) in common wheat, and improving PHS tolerance is a crucial factor for wheat breeding. Spelt wheat is always used as the donor parent to improve resistance and quality in wheat breeding: however, the roles of the Vp-1 genes in spelt wheat and their relationship to common wheat remain uncertain. The current study aimed to isolate and characterise Vp-1 haplotypes in spelt wheat (Triticum aestivum subsp. spelta).

RESULTS

In spelt wheat, a total of eight new Vp-1 haplotypes were identified: TaVp-1Ap, TaVp-1Aq and TaVp-1Ar in the A genome; TaVp-1Bj, TaVp-1Bh and TaVp-1Bi in the B genome; and TaVp-1Da and TaVp-1Db in the D genome. According to RT-PCR results, correctly spliced transcripts were more highly expressed in some haplotypes than in others, and their expression was highly associated with their distinct responsiveness to abscisic acid (ABA) exposure. The mis-splicing of Vp-1 transcripts and several indel variations detected in spelt wheat appear to have been retained through the hybridisation process.

CONCLUSION

Certain haplotypes detected in spelt wheat might be valuable in the breeding and selection of germplasm to improve PHS issues in wheat. © 2016 Society of Chemical Industry.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Feng Y
College of Life Sciences, Inner Mongolia Agricultural University, 306 Zhaowuda Road, Hohhot, 010018, Inner Mongolia, China.
Qu R
College of Life Sciences, Inner Mongolia Agricultural University, 306 Zhaowuda Road, Hohhot, 010018, Inner Mongolia, China.
Liu S
College of Life Sciences, Inner Mongolia Agricultural University, 306 Zhaowuda Road, Hohhot, 010018, Inner Mongolia, China.
Yang Y
College of Life Sciences, Inner Mongolia Agricultural University, 306 Zhaowuda Road, Hohhot, 010018, Inner Mongolia, China.

MeSH

Abscisic AcidAllelesGenes, PlantGenome, PlantGerminationHaplotypesHybridization, GeneticPlant BreedingPlant ProteinsTriticum

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27060998

Citation

Feng, Yumei, et al. "Rich Haplotypes of Viviparous-1 in Triticum Aestivum Subsp. Spelta With Different Abscisic Acid Sensitivities." Journal of the Science of Food and Agriculture, vol. 97, no. 2, 2017, pp. 497-504.
Feng Y, Qu R, Liu S, et al. Rich haplotypes of Viviparous-1 in Triticum aestivum subsp. spelta with different abscisic acid sensitivities. J Sci Food Agric. 2017;97(2):497-504.
Feng, Y., Qu, R., Liu, S., & Yang, Y. (2017). Rich haplotypes of Viviparous-1 in Triticum aestivum subsp. spelta with different abscisic acid sensitivities. Journal of the Science of Food and Agriculture, 97(2), 497-504. https://doi.org/10.1002/jsfa.7751
Feng Y, et al. Rich Haplotypes of Viviparous-1 in Triticum Aestivum Subsp. Spelta With Different Abscisic Acid Sensitivities. J Sci Food Agric. 2017;97(2):497-504. PubMed PMID: 27060998.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rich haplotypes of Viviparous-1 in Triticum aestivum subsp. spelta with different abscisic acid sensitivities. AU - Feng,Yumei, AU - Qu,Ruoduan, AU - Liu,Simeng, AU - Yang,Yan, Y1 - 2016/05/12/ PY - 2015/09/26/received PY - 2016/03/23/revised PY - 2016/03/31/accepted PY - 2016/4/12/pubmed PY - 2017/5/26/medline PY - 2016/4/11/entrez KW - RT-PCR KW - Vp-1 KW - allele KW - pre-harvest sprouting KW - spelt wheat SP - 497 EP - 504 JF - Journal of the science of food and agriculture JO - J Sci Food Agric VL - 97 IS - 2 N2 - BACKGROUND: Viviparous-1 (Vp-1) is a major gene affecting pre-harvest sprouting (PHS) in common wheat, and improving PHS tolerance is a crucial factor for wheat breeding. Spelt wheat is always used as the donor parent to improve resistance and quality in wheat breeding: however, the roles of the Vp-1 genes in spelt wheat and their relationship to common wheat remain uncertain. The current study aimed to isolate and characterise Vp-1 haplotypes in spelt wheat (Triticum aestivum subsp. spelta). RESULTS: In spelt wheat, a total of eight new Vp-1 haplotypes were identified: TaVp-1Ap, TaVp-1Aq and TaVp-1Ar in the A genome; TaVp-1Bj, TaVp-1Bh and TaVp-1Bi in the B genome; and TaVp-1Da and TaVp-1Db in the D genome. According to RT-PCR results, correctly spliced transcripts were more highly expressed in some haplotypes than in others, and their expression was highly associated with their distinct responsiveness to abscisic acid (ABA) exposure. The mis-splicing of Vp-1 transcripts and several indel variations detected in spelt wheat appear to have been retained through the hybridisation process. CONCLUSION: Certain haplotypes detected in spelt wheat might be valuable in the breeding and selection of germplasm to improve PHS issues in wheat. © 2016 Society of Chemical Industry. SN - 1097-0010 UR - https://www.unboundmedicine.com/medline/citation/27060998/Rich_haplotypes_of_Viviparous_1_in_Triticum_aestivum_subsp__spelta_with_different_abscisic_acid_sensitivities_ DB - PRIME DP - Unbound Medicine ER -