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Translational machinery of mitochondrial mRNA is promoted by physical activity in Western diet-induced obese mice.
Acta Physiol (Oxf) 2016; 218(3):167-177AP

Abstract

AIM

Mitochondria-encoded proteins are necessary for oxidative phosphorylation; however, no report has examined how physical activity (PA) and obesity affect mitochondrial mRNA translation machinery. Our purpose was to determine whether Western diet (WD)-induced obesity and voluntary wheel running (VWR) impact mitochondrial mRNA translation machinery and whether expression of this machinery is dictated by oxidative phenotype.

METHODS

Obesity was induced with 8-wk WD feeding, and in the final 4 wks, half of mice were allowed VWR. Mitochondrial mRNA translation machinery including initiation factors (mtIF2/3), elongation factor Tu (TUFM) and translational activator (TACO1), and mitochondria-encoded proteins (CytB and ND4) was assessed by immunoblotting. The relation of mitochondrial mRNA translation to muscle oxidative phenotype was assessed using PGC-1α transgenic overexpression (MCK-PGC-1α vs. wild-type mice) and comparing across muscle groups in wild-type mice.

RESULTS

mtIF3 and TACO1 proteins were ~45% greater in VWR than sedentary (SED), and TACO1 and mtIF2 proteins were ~60% and 125% greater in WD than normal chow (NC). TUFM protein was ~50% lower in WD-SED than NC-SED, but ~50% greater in WD-VWR compared to NC-SED. CytB and ND4 were ~40% greater in VWR and ND4 was twofold greater with WD. TUFM, TACO1, ND4 and CytB were greater in MCK-PGC-1α compared to wild-type, and mtIF2/3 contents were not different. In oxidative muscle (soleus), mitochondrial translation machinery was elevated compared to mixed (gastrocnemius) or glycolytic (extensor digitorum longus) muscles.

CONCLUSION

These data suggest a novel mechanism promoting mitochondrial function by translation of mitochondrial protein following PA. This may act to promote muscle health by PA in obesity.

Authors+Show Affiliations

Integrative Muscle Metabolism Laboratory, Human Performance Laboratory, Department of Health, Human Performance and Recreation, University of Arkansas, Fayetteville, AR, USA.Integrative Muscle Metabolism Laboratory, Human Performance Laboratory, Department of Health, Human Performance and Recreation, University of Arkansas, Fayetteville, AR, USA.Integrative Muscle Metabolism Laboratory, Human Performance Laboratory, Department of Health, Human Performance and Recreation, University of Arkansas, Fayetteville, AR, USA.Exercise Muscle Biology Laboratory, Human Performance Laboratory, Department of Health, Human Performance and Recreation, University of Arkansas, Fayetteville, AR, USA.Exercise Muscle Biology Laboratory, Human Performance Laboratory, Department of Health, Human Performance and Recreation, University of Arkansas, Fayetteville, AR, USA.Exercise Muscle Biology Laboratory, Human Performance Laboratory, Department of Health, Human Performance and Recreation, University of Arkansas, Fayetteville, AR, USA.Integrative Muscle Metabolism Laboratory, Human Performance Laboratory, Department of Health, Human Performance and Recreation, University of Arkansas, Fayetteville, AR, USA. npgreene@uark.edu.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27061106

Citation

Lee, D E., et al. "Translational Machinery of Mitochondrial mRNA Is Promoted By Physical Activity in Western Diet-induced Obese Mice." Acta Physiologica (Oxford, England), vol. 218, no. 3, 2016, pp. 167-177.
Lee DE, Brown JL, Rosa ME, et al. Translational machinery of mitochondrial mRNA is promoted by physical activity in Western diet-induced obese mice. Acta Physiol (Oxf). 2016;218(3):167-177.
Lee, D. E., Brown, J. L., Rosa, M. E., Brown, L. A., Perry, R. A., Washington, T. A., & Greene, N. P. (2016). Translational machinery of mitochondrial mRNA is promoted by physical activity in Western diet-induced obese mice. Acta Physiologica (Oxford, England), 218(3), pp. 167-177. doi:10.1111/apha.12687.
Lee DE, et al. Translational Machinery of Mitochondrial mRNA Is Promoted By Physical Activity in Western Diet-induced Obese Mice. Acta Physiol (Oxf). 2016;218(3):167-177. PubMed PMID: 27061106.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Translational machinery of mitochondrial mRNA is promoted by physical activity in Western diet-induced obese mice. AU - Lee,D E, AU - Brown,J L, AU - Rosa,M E, AU - Brown,L A, AU - Perry,R A, AU - Washington,T A, AU - Greene,N P, Y1 - 2016/04/25/ PY - 2015/11/05/received PY - 2016/04/06/revised PY - 2016/04/07/accepted PY - 2016/4/12/pubmed PY - 2017/10/25/medline PY - 2016/4/11/entrez KW - PGC-1α KW - TACO1 KW - TUFM KW - exercise and obesity KW - mtIFs SP - 167 EP - 177 JF - Acta physiologica (Oxford, England) JO - Acta Physiol (Oxf) VL - 218 IS - 3 N2 - AIM: Mitochondria-encoded proteins are necessary for oxidative phosphorylation; however, no report has examined how physical activity (PA) and obesity affect mitochondrial mRNA translation machinery. Our purpose was to determine whether Western diet (WD)-induced obesity and voluntary wheel running (VWR) impact mitochondrial mRNA translation machinery and whether expression of this machinery is dictated by oxidative phenotype. METHODS: Obesity was induced with 8-wk WD feeding, and in the final 4 wks, half of mice were allowed VWR. Mitochondrial mRNA translation machinery including initiation factors (mtIF2/3), elongation factor Tu (TUFM) and translational activator (TACO1), and mitochondria-encoded proteins (CytB and ND4) was assessed by immunoblotting. The relation of mitochondrial mRNA translation to muscle oxidative phenotype was assessed using PGC-1α transgenic overexpression (MCK-PGC-1α vs. wild-type mice) and comparing across muscle groups in wild-type mice. RESULTS: mtIF3 and TACO1 proteins were ~45% greater in VWR than sedentary (SED), and TACO1 and mtIF2 proteins were ~60% and 125% greater in WD than normal chow (NC). TUFM protein was ~50% lower in WD-SED than NC-SED, but ~50% greater in WD-VWR compared to NC-SED. CytB and ND4 were ~40% greater in VWR and ND4 was twofold greater with WD. TUFM, TACO1, ND4 and CytB were greater in MCK-PGC-1α compared to wild-type, and mtIF2/3 contents were not different. In oxidative muscle (soleus), mitochondrial translation machinery was elevated compared to mixed (gastrocnemius) or glycolytic (extensor digitorum longus) muscles. CONCLUSION: These data suggest a novel mechanism promoting mitochondrial function by translation of mitochondrial protein following PA. This may act to promote muscle health by PA in obesity. SN - 1748-1716 UR - https://www.unboundmedicine.com/medline/citation/27061106/Translational_machinery_of_mitochondrial_mRNA_is_promoted_by_physical_activity_in_Western_diet_induced_obese_mice_ L2 - https://doi.org/10.1111/apha.12687 DB - PRIME DP - Unbound Medicine ER -