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Cytotoxicity of 15 Cameroonian medicinal plants against drug sensitive and multi-drug resistant cancer cells.
J Ethnopharmacol 2016; 186:196-204JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Cameroonian medicinal plants are traditionally used to treat many ailments, including cancer and related diseases. Cancer is characterized as a condition with complex signs and symptoms. It has been recommended that ethnopharmacological usages such as immune and skin disorders, inflammatory, infectious, parasitic and viral diseases should be taken into account when selecting plants for anticancer screenings, since these reflect disease states bearing relevance to cancer or cancer-like symptoms.

AIM OF THE STUDY

The present study aims at investigating 20 methanol extracts from 15 Cameroonian medicinal plants on a panel of human cancer cell lines, including various drug-resistant phenotypes. Possible modes of action of the of the most active plant were analyzed.

MATERIALS AND METHODS

Methanol extracts from different plant parts (leaves, bark, roots, fruits or whole plant) were evaluated for their cytotoxicity using resazurin reduction assay on a panel of nine sensitive and multi-drug resistant (MDR) cancer cell lines. Cell cycle, apoptosis, mitochondrial membrane potential (MMP), and reactive oxygen species (ROS) were measured by flow cytometry.

RESULTS

Prescreening of extracts at 80µg/mL showed that 6 extracts out of 20 inhibited more than 50% proliferation of leukemia CCRF-CEM cells; these include extracts from Anthocleista schweinfurthii fruits (ASF; 48.28%), Morus mesozygia bark (MMB; 42.76%), Nauclea latifolia bark (NLB; 38.75%), Tridesmostemon omphalocarpoides bark (TOB; 38.53%), Nauclea latifolia leaves (NLL; 35.17%) and Erythrina sigmoidea bark (ESB; 33.77%). Subsequent investigations revealed IC50 values below or around 20µg/mL for extracts from MMB, NLB, NLL and ESB towards sensitive CCRF-CEM cells and its resistant P-glycoprotein over-expressing subline CEM/ADR5000. The best extract, ESB also displayed IC50 values below 20µg/mL colon carcinoma HCT116 (p53(+/+)) cells with an IC50 value of 19.63µg/mL and it resistant p53 knockout subline HCT116 (p53(-)(/-)) with an IC50 value of 16.22µg/mL.

CONCLUSION

Erythrina sigmoidea, Anthocleista schweinfurthii, Morus mesozygia, Nauclea latifolia, Tridesmostemon omphalocarpoides used in African traditional medicine are good cytotoxic plants that can be exploited to develop phytomedicine to fight cancers including MDR phenotypes.

Authors+Show Affiliations

Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, University of Mainz, Staudinger Weg 5, 55128 Mainz, Germany; Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon. Electronic address: kuetevictor@yahoo.fr.Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon.Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon.Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, University of Mainz, Staudinger Weg 5, 55128 Mainz, Germany.Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, University of Mainz, Staudinger Weg 5, 55128 Mainz, Germany. Electronic address: efferth@uni-mainz.de.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27063984

Citation

Kuete, Victor, et al. "Cytotoxicity of 15 Cameroonian Medicinal Plants Against Drug Sensitive and Multi-drug Resistant Cancer Cells." Journal of Ethnopharmacology, vol. 186, 2016, pp. 196-204.
Kuete V, Djeussi DE, Mbaveng AT, et al. Cytotoxicity of 15 Cameroonian medicinal plants against drug sensitive and multi-drug resistant cancer cells. J Ethnopharmacol. 2016;186:196-204.
Kuete, V., Djeussi, D. E., Mbaveng, A. T., Zeino, M., & Efferth, T. (2016). Cytotoxicity of 15 Cameroonian medicinal plants against drug sensitive and multi-drug resistant cancer cells. Journal of Ethnopharmacology, 186, pp. 196-204. doi:10.1016/j.jep.2016.04.001.
Kuete V, et al. Cytotoxicity of 15 Cameroonian Medicinal Plants Against Drug Sensitive and Multi-drug Resistant Cancer Cells. J Ethnopharmacol. 2016 Jun 20;186:196-204. PubMed PMID: 27063984.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cytotoxicity of 15 Cameroonian medicinal plants against drug sensitive and multi-drug resistant cancer cells. AU - Kuete,Victor, AU - Djeussi,Doriane E, AU - Mbaveng,Armelle T, AU - Zeino,Maen, AU - Efferth,Thomas, Y1 - 2016/04/05/ PY - 2016/01/20/received PY - 2016/04/02/revised PY - 2016/04/03/accepted PY - 2016/4/12/entrez PY - 2016/4/12/pubmed PY - 2017/3/4/medline KW - Apoptosis KW - Cameroon KW - Cytotoxicity KW - Erythrina sigmoidea KW - Medicinal plants SP - 196 EP - 204 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 186 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Cameroonian medicinal plants are traditionally used to treat many ailments, including cancer and related diseases. Cancer is characterized as a condition with complex signs and symptoms. It has been recommended that ethnopharmacological usages such as immune and skin disorders, inflammatory, infectious, parasitic and viral diseases should be taken into account when selecting plants for anticancer screenings, since these reflect disease states bearing relevance to cancer or cancer-like symptoms. AIM OF THE STUDY: The present study aims at investigating 20 methanol extracts from 15 Cameroonian medicinal plants on a panel of human cancer cell lines, including various drug-resistant phenotypes. Possible modes of action of the of the most active plant were analyzed. MATERIALS AND METHODS: Methanol extracts from different plant parts (leaves, bark, roots, fruits or whole plant) were evaluated for their cytotoxicity using resazurin reduction assay on a panel of nine sensitive and multi-drug resistant (MDR) cancer cell lines. Cell cycle, apoptosis, mitochondrial membrane potential (MMP), and reactive oxygen species (ROS) were measured by flow cytometry. RESULTS: Prescreening of extracts at 80µg/mL showed that 6 extracts out of 20 inhibited more than 50% proliferation of leukemia CCRF-CEM cells; these include extracts from Anthocleista schweinfurthii fruits (ASF; 48.28%), Morus mesozygia bark (MMB; 42.76%), Nauclea latifolia bark (NLB; 38.75%), Tridesmostemon omphalocarpoides bark (TOB; 38.53%), Nauclea latifolia leaves (NLL; 35.17%) and Erythrina sigmoidea bark (ESB; 33.77%). Subsequent investigations revealed IC50 values below or around 20µg/mL for extracts from MMB, NLB, NLL and ESB towards sensitive CCRF-CEM cells and its resistant P-glycoprotein over-expressing subline CEM/ADR5000. The best extract, ESB also displayed IC50 values below 20µg/mL colon carcinoma HCT116 (p53(+/+)) cells with an IC50 value of 19.63µg/mL and it resistant p53 knockout subline HCT116 (p53(-)(/-)) with an IC50 value of 16.22µg/mL. CONCLUSION: Erythrina sigmoidea, Anthocleista schweinfurthii, Morus mesozygia, Nauclea latifolia, Tridesmostemon omphalocarpoides used in African traditional medicine are good cytotoxic plants that can be exploited to develop phytomedicine to fight cancers including MDR phenotypes. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/27063984/Cytotoxicity_of_15_Cameroonian_medicinal_plants_against_drug_sensitive_and_multi_drug_resistant_cancer_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(16)30192-1 DB - PRIME DP - Unbound Medicine ER -