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Influence of Light and Temperature on Gene Expression Leading to Accumulation of Specific Flavonol Glycosides and Hydroxycinnamic Acid Derivatives in Kale (Brassica oleracea var. sabellica).
Front Plant Sci. 2016; 7:326.FP

Abstract

Light intensity and temperature are very important signals for the regulation of plant growth and development. Plants subjected to less favorable light or temperature conditions often respond with accumulation of secondary metabolites. Some of these metabolites have been identified as bioactive compounds, considered to exert positive effects on human health when consumed regularly. In order to test a typical range of growth parameters for the winter crop Brassica oleracea var. sabellica, plants were grown either at 400 μmol m(-2) s(-1) or 100 μmol m(-2) s(-1) at 10°C, or at 400 μmol m(-2) s(-1) with 5 or 15°C. The higher light intensity overall increased flavonol content of leaves, favoring the main quercetin glycosides, a caffeic acid monoacylated kaempferol triglycoside, and disinapoyl-gentiobiose. The higher temperature mainly increased the hydroxycinnamic acid derivative disinapoyl-gentiobiose, while at lower temperature synthesis is in favor of very complex sinapic acid acylated flavonol tetraglycosides such as kaempferol-3-O-sinapoyl-sophoroside-7-O-diglucoside. A global analysis of light and temperature dependent alterations of gene expression in B. oleracea var. sabellica leaves was performed with the most comprehensive Brassica microarray. When compared to the light experiment much less genes were differentially expressed in kale leaves grown at 5 or 15°C. A structured evaluation of differentially expressed genes revealed the expected enrichment in the functional categories of e.g. protein degradation at different light intensities or phytohormone metabolism at different temperature. Genes of the secondary metabolism namely phenylpropanoids are significantly enriched with both treatments. Thus, the genome of B. oleracea was screened for predicted genes putatively involved in the biosynthesis of flavonoids and hydroxycinnamic acid derivatives. All identified B. oleracea genes were analyzed for their most specific 60-mer oligonucleotides present on the 2 × 105 K format Brassica microarray. Expression differences were correlated to the structure-dependent response of flavonoid glycosides and hydroxycinnamic acid derivatives to alterations in either light or temperature. The altered metabolite accumulation was mainly reflected on gene expression level of core biosynthetic pathway genes and gave further hints to an isoform specific functional specialization.

Authors+Show Affiliations

Leibniz Institute of Vegetable and Ornamental Crops Groβbeeren/Erfurt e.V. Groβbeeren, Germany.Leibniz Institute of Vegetable and Ornamental Crops Groβbeeren/Erfurt e.V. Groβbeeren, Germany.Leibniz Institute of Vegetable and Ornamental Crops Groβbeeren/Erfurt e.V. Groβbeeren, Germany.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27066016

Citation

Neugart, Susanne, et al. "Influence of Light and Temperature On Gene Expression Leading to Accumulation of Specific Flavonol Glycosides and Hydroxycinnamic Acid Derivatives in Kale (Brassica Oleracea Var. Sabellica)." Frontiers in Plant Science, vol. 7, 2016, p. 326.
Neugart S, Krumbein A, Zrenner R. Influence of Light and Temperature on Gene Expression Leading to Accumulation of Specific Flavonol Glycosides and Hydroxycinnamic Acid Derivatives in Kale (Brassica oleracea var. sabellica). Front Plant Sci. 2016;7:326.
Neugart, S., Krumbein, A., & Zrenner, R. (2016). Influence of Light and Temperature on Gene Expression Leading to Accumulation of Specific Flavonol Glycosides and Hydroxycinnamic Acid Derivatives in Kale (Brassica oleracea var. sabellica). Frontiers in Plant Science, 7, 326. https://doi.org/10.3389/fpls.2016.00326
Neugart S, Krumbein A, Zrenner R. Influence of Light and Temperature On Gene Expression Leading to Accumulation of Specific Flavonol Glycosides and Hydroxycinnamic Acid Derivatives in Kale (Brassica Oleracea Var. Sabellica). Front Plant Sci. 2016;7:326. PubMed PMID: 27066016.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Influence of Light and Temperature on Gene Expression Leading to Accumulation of Specific Flavonol Glycosides and Hydroxycinnamic Acid Derivatives in Kale (Brassica oleracea var. sabellica). AU - Neugart,Susanne, AU - Krumbein,Angelika, AU - Zrenner,Rita, Y1 - 2016/03/30/ PY - 2015/12/10/received PY - 2016/03/03/accepted PY - 2016/4/12/entrez PY - 2016/4/12/pubmed PY - 2016/4/12/medline KW - Brassica KW - flavonol glycosides KW - gene expression KW - microarray KW - photosynthetically active radiation (PAR) KW - temperature SP - 326 EP - 326 JF - Frontiers in plant science JO - Front Plant Sci VL - 7 N2 - Light intensity and temperature are very important signals for the regulation of plant growth and development. Plants subjected to less favorable light or temperature conditions often respond with accumulation of secondary metabolites. Some of these metabolites have been identified as bioactive compounds, considered to exert positive effects on human health when consumed regularly. In order to test a typical range of growth parameters for the winter crop Brassica oleracea var. sabellica, plants were grown either at 400 μmol m(-2) s(-1) or 100 μmol m(-2) s(-1) at 10°C, or at 400 μmol m(-2) s(-1) with 5 or 15°C. The higher light intensity overall increased flavonol content of leaves, favoring the main quercetin glycosides, a caffeic acid monoacylated kaempferol triglycoside, and disinapoyl-gentiobiose. The higher temperature mainly increased the hydroxycinnamic acid derivative disinapoyl-gentiobiose, while at lower temperature synthesis is in favor of very complex sinapic acid acylated flavonol tetraglycosides such as kaempferol-3-O-sinapoyl-sophoroside-7-O-diglucoside. A global analysis of light and temperature dependent alterations of gene expression in B. oleracea var. sabellica leaves was performed with the most comprehensive Brassica microarray. When compared to the light experiment much less genes were differentially expressed in kale leaves grown at 5 or 15°C. A structured evaluation of differentially expressed genes revealed the expected enrichment in the functional categories of e.g. protein degradation at different light intensities or phytohormone metabolism at different temperature. Genes of the secondary metabolism namely phenylpropanoids are significantly enriched with both treatments. Thus, the genome of B. oleracea was screened for predicted genes putatively involved in the biosynthesis of flavonoids and hydroxycinnamic acid derivatives. All identified B. oleracea genes were analyzed for their most specific 60-mer oligonucleotides present on the 2 × 105 K format Brassica microarray. Expression differences were correlated to the structure-dependent response of flavonoid glycosides and hydroxycinnamic acid derivatives to alterations in either light or temperature. The altered metabolite accumulation was mainly reflected on gene expression level of core biosynthetic pathway genes and gave further hints to an isoform specific functional specialization. SN - 1664-462X UR - https://www.unboundmedicine.com/medline/citation/27066016/Influence_of_Light_and_Temperature_on_Gene_Expression_Leading_to_Accumulation_of_Specific_Flavonol_Glycosides_and_Hydroxycinnamic_Acid_Derivatives_in_Kale__Brassica_oleracea_var__sabellica__ L2 - https://doi.org/10.3389/fpls.2016.00326 DB - PRIME DP - Unbound Medicine ER -
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