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Enhanced activity of hormone sensitive lipase (HSL) in mesenteric but not epididymal fat correlates with higher production of epinephrine in mesenteric adipocytes in rat model of cachectic rheumatoid arthritis.
Autoimmunity. 2016 06; 49(4):268-76.A

Abstract

Cachectic rheumatoid arthritis, the less frequent form of the disease, is associated with loss of fat mass and often more severe course of the disease. Its experimental model represents rat adjuvant arthritis (AA) characterized by edema, lack of appetite, sharp body weight and fat loss. As individual fat depots display functional differences, here we studied lipolytic activity and sensitivity to lipolytic stimuli of nodeless epididymal fat (eWAT) and perinodal mesenteric fat (mWAT) depots at the peak of AA. We also examined changes in catecholamine and cytokine levels involved in lipolysis in plasma and/or isolated adipocytes from both WATs to identify the contribution of local, adipocyte-based processes and/or systemic events to adiposity loss in cachectic rheumatoid arthritis. AA was induced to male Lewis rats by complete Freund's adjuvant. Groups of ad libitum-fed and pair-fed controls were used to distinguish the effects of food restriction from inflammation-induced cachexia. Adipose triglyceride lipase (ATGL), hormone-sensitive lipase (HSL) and its phosphorylated form (pHSL) were analyzed by western blot. CRP and catecholamine levels in plasma or adipocyte lysates were determined using ELISA kits. Cytokine-induced neutrophil chemoattractant-1 (CINC-1/CXCL1), monocyte chemoattractant protein-1 (MCP-1/CCL2), IL-1β, IL-6, IL-10 and leptin in adipocyte lysate were analyzed by quantitative protein microarray. Plasma glycerol and FFA were measured spectrophotometrically. AA rats developed severe cachexia, with lower adiposity in mWAT compared to normal and pair-fed controls, whereas in eWAT the adiposity was similarly reduced in AA and pair-fed groups. ATGL levels in both WATs were not affected by AA or pair feeding. AA upregulated levels of HSL, pHSL and pHSL/HSL ratio in mWAT, whereas none of these parameters has changed in eWAT of AA rats or in either WATs of pair-fed rats. In AA rats plasma glycerol was elevated, whereas FFA concentration was reduced. Plasma norepinephrine and epinephrine were increased in AA compared with both groups of controls. In eWAT adipocytes, AA but not pair feeding, upregulated norepinephrine levels. In mWAT adipocytes, AA rats showed higher epinephrine levels than pair-fed controls. Leptin levels in both WATs were depleted in AA animals in accordance with body weight loss. None of the measured cytokines in eWAT and mWAT was enhanced. Our results demonstrate augmented lipolytic activity in mWAT and not eWAT during cachectic arthritis. The adipocyte-derived cytokines do not seem to contribute to activated lipolysis. We first demonstrated enhanced presence of norepinephrine in perinodal adipocytes that may contribute to the regulation of local lipolytic activity by auto/paracrine fashion and thus provide independent fuel supply to activated lymph nodes.

Authors+Show Affiliations

a Department of Normal , Pathological, and Clinical Physiology, Third Faculty of Medicine, Charles University , Prague , Czech Republic and. b Institute of Experimental Endocrinology, Slovak Academy of Sciences , Bratislava , Slovakia.b Institute of Experimental Endocrinology, Slovak Academy of Sciences , Bratislava , Slovakia.a Department of Normal , Pathological, and Clinical Physiology, Third Faculty of Medicine, Charles University , Prague , Czech Republic and.a Department of Normal , Pathological, and Clinical Physiology, Third Faculty of Medicine, Charles University , Prague , Czech Republic and. b Institute of Experimental Endocrinology, Slovak Academy of Sciences , Bratislava , Slovakia.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27068752

Citation

Stofkova, Andrea, et al. "Enhanced Activity of Hormone Sensitive Lipase (HSL) in Mesenteric but Not Epididymal Fat Correlates With Higher Production of Epinephrine in Mesenteric Adipocytes in Rat Model of Cachectic Rheumatoid Arthritis." Autoimmunity, vol. 49, no. 4, 2016, pp. 268-76.
Stofkova A, Krskova K, Vaculin S, et al. Enhanced activity of hormone sensitive lipase (HSL) in mesenteric but not epididymal fat correlates with higher production of epinephrine in mesenteric adipocytes in rat model of cachectic rheumatoid arthritis. Autoimmunity. 2016;49(4):268-76.
Stofkova, A., Krskova, K., Vaculin, S., & Jurcovicova, J. (2016). Enhanced activity of hormone sensitive lipase (HSL) in mesenteric but not epididymal fat correlates with higher production of epinephrine in mesenteric adipocytes in rat model of cachectic rheumatoid arthritis. Autoimmunity, 49(4), 268-76. https://doi.org/10.3109/08916934.2016.1164145
Stofkova A, et al. Enhanced Activity of Hormone Sensitive Lipase (HSL) in Mesenteric but Not Epididymal Fat Correlates With Higher Production of Epinephrine in Mesenteric Adipocytes in Rat Model of Cachectic Rheumatoid Arthritis. Autoimmunity. 2016;49(4):268-76. PubMed PMID: 27068752.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Enhanced activity of hormone sensitive lipase (HSL) in mesenteric but not epididymal fat correlates with higher production of epinephrine in mesenteric adipocytes in rat model of cachectic rheumatoid arthritis. AU - Stofkova,Andrea, AU - Krskova,Katarina, AU - Vaculin,Simon, AU - Jurcovicova,Jana, Y1 - 2016/04/12/ PY - 2016/4/13/entrez PY - 2016/4/14/pubmed PY - 2017/12/23/medline KW - Adipocyte adrenaline KW - cachectic adjuvant arthritis KW - epididymal fat KW - lipolysis KW - mesenteric fat KW - rat SP - 268 EP - 76 JF - Autoimmunity JO - Autoimmunity VL - 49 IS - 4 N2 - Cachectic rheumatoid arthritis, the less frequent form of the disease, is associated with loss of fat mass and often more severe course of the disease. Its experimental model represents rat adjuvant arthritis (AA) characterized by edema, lack of appetite, sharp body weight and fat loss. As individual fat depots display functional differences, here we studied lipolytic activity and sensitivity to lipolytic stimuli of nodeless epididymal fat (eWAT) and perinodal mesenteric fat (mWAT) depots at the peak of AA. We also examined changes in catecholamine and cytokine levels involved in lipolysis in plasma and/or isolated adipocytes from both WATs to identify the contribution of local, adipocyte-based processes and/or systemic events to adiposity loss in cachectic rheumatoid arthritis. AA was induced to male Lewis rats by complete Freund's adjuvant. Groups of ad libitum-fed and pair-fed controls were used to distinguish the effects of food restriction from inflammation-induced cachexia. Adipose triglyceride lipase (ATGL), hormone-sensitive lipase (HSL) and its phosphorylated form (pHSL) were analyzed by western blot. CRP and catecholamine levels in plasma or adipocyte lysates were determined using ELISA kits. Cytokine-induced neutrophil chemoattractant-1 (CINC-1/CXCL1), monocyte chemoattractant protein-1 (MCP-1/CCL2), IL-1β, IL-6, IL-10 and leptin in adipocyte lysate were analyzed by quantitative protein microarray. Plasma glycerol and FFA were measured spectrophotometrically. AA rats developed severe cachexia, with lower adiposity in mWAT compared to normal and pair-fed controls, whereas in eWAT the adiposity was similarly reduced in AA and pair-fed groups. ATGL levels in both WATs were not affected by AA or pair feeding. AA upregulated levels of HSL, pHSL and pHSL/HSL ratio in mWAT, whereas none of these parameters has changed in eWAT of AA rats or in either WATs of pair-fed rats. In AA rats plasma glycerol was elevated, whereas FFA concentration was reduced. Plasma norepinephrine and epinephrine were increased in AA compared with both groups of controls. In eWAT adipocytes, AA but not pair feeding, upregulated norepinephrine levels. In mWAT adipocytes, AA rats showed higher epinephrine levels than pair-fed controls. Leptin levels in both WATs were depleted in AA animals in accordance with body weight loss. None of the measured cytokines in eWAT and mWAT was enhanced. Our results demonstrate augmented lipolytic activity in mWAT and not eWAT during cachectic arthritis. The adipocyte-derived cytokines do not seem to contribute to activated lipolysis. We first demonstrated enhanced presence of norepinephrine in perinodal adipocytes that may contribute to the regulation of local lipolytic activity by auto/paracrine fashion and thus provide independent fuel supply to activated lymph nodes. SN - 1607-842X UR - https://www.unboundmedicine.com/medline/citation/27068752/Enhanced_activity_of_hormone_sensitive_lipase__HSL__in_mesenteric_but_not_epididymal_fat_correlates_with_higher_production_of_epinephrine_in_mesenteric_adipocytes_in_rat_model_of_cachectic_rheumatoid_arthritis_ L2 - http://www.tandfonline.com/doi/full/10.3109/08916934.2016.1164145 DB - PRIME DP - Unbound Medicine ER -