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The transcardiac gradient of cardio-microRNAs in the failing heart.
Eur J Heart Fail. 2016 08; 18(8):1000-8.EJ

Abstract

AIMS

Differential microRNA expression in peripheral blood has been observed in patients with heart failure, suggesting their value as potential biomarkers and likely contributors to disease mechanisms. In the present study, we aimed to evaluate the transcardiac gradient of 84 cardio-microRNAs in healthy and failing hearts to determine which microRNAs are released or absorbed by the myocardium in heart failure.

METHODS AND RESULTS

Eight healthy volunteers and nine patients with congestive heart failure were included. Arterial and coronary sinus blood samples were collected, and microRNAs were extracted. The expression of microRNAs was analysed using real-time PCR by the miScript miRNA PCR Array Human Cardiovascular Disease. In coronary sinus samples, the microRNAs miR-16-5p, miR-27a-3p, miR-27b-3p, miR-29b-3p, miR-29c-3p, miR-30e-5p, miR-92a-3p, miR-125b-5p, miR-140-5p, miR-195-5p, miR-424-5p, and miR-451a were significantly down-regulated, and let-7a-5p, let-7c-5p, let-7e-5p, miR-23b-3p, miR-107, miR-155-5p, miR-181a-5p, miR-181b-5p and miR-320a were up-regulated in heart failure. Left ventricular filling pressure was negatively correlated with miR-195, miR-16, miR-29b-3p, miR-29c-3p, miR-451a, and miR-92a-3p. The failing heart released let-7b-5p, let-7c-5p, let-7e-5p, miR-122-5p, and miR-21-5p, and absorbed miR-16-5p, miR-17-5p, miR-27a-3p, miR-30a-5p, miR-30d-5p, miR-30e-5p, miR-130a-3p, miR-140-5p, miR-199a-5p, and miR-451a. In silico analyses suggest that the transcardiac gradient of microRNAs in heart failure may target pathways related to heart disease.

CONCLUSION

We determined the transcardiac gradient of cardio-microRNAs in failing hearts, which supports the use of these microRNAs as potential biomarkers. The microRNAs described here may have a role in the pathophysiology of heart failure as they might be involved in pathways related to disease progression, including fibrosis.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Marques FZ
Heart Failure Research Group, Baker IDI Heart and Diabetes Research Institute VIC, Australia.
Vizi D
Heart Centre, Alfred Hospital, Melbourne, VIC, Australia.
Khammy O
Heart Failure Research Group, Baker IDI Heart and Diabetes Research Institute VIC, Australia.
Mariani JA
Heart Failure Research Group, Baker IDI Heart and Diabetes Research Institute VIC, Australia. Heart Centre, Alfred Hospital, Melbourne, VIC, Australia.
Kaye DM
Heart Failure Research Group, Baker IDI Heart and Diabetes Research Institute VIC, Australia. Heart Centre, Alfred Hospital, Melbourne, VIC, Australia.

MeSH

AdultAgedArteriesBlood Specimen CollectionCase-Control StudiesComputer SimulationCoronary SinusDiastoleFemaleHeart FailureHumansMaleMicroRNAsMiddle AgedPressureSeverity of Illness IndexUp-Regulation

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27072074

Clinical Trial Links

BIO-2-HEART: Identification of Biomarkers in T2DM and Heart Failure

Citation

Marques, Francine Z., et al. "The Transcardiac Gradient of cardio-microRNAs in the Failing Heart." European Journal of Heart Failure, vol. 18, no. 8, 2016, pp. 1000-8.
Marques FZ, Vizi D, Khammy O, et al. The transcardiac gradient of cardio-microRNAs in the failing heart. Eur J Heart Fail. 2016;18(8):1000-8.
Marques, F. Z., Vizi, D., Khammy, O., Mariani, J. A., & Kaye, D. M. (2016). The transcardiac gradient of cardio-microRNAs in the failing heart. European Journal of Heart Failure, 18(8), 1000-8. https://doi.org/10.1002/ejhf.517
Marques FZ, et al. The Transcardiac Gradient of cardio-microRNAs in the Failing Heart. Eur J Heart Fail. 2016;18(8):1000-8. PubMed PMID: 27072074.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The transcardiac gradient of cardio-microRNAs in the failing heart. AU - Marques,Francine Z, AU - Vizi,Donna, AU - Khammy,Ouda, AU - Mariani,Justin A, AU - Kaye,David M, Y1 - 2016/04/12/ PY - 2015/10/04/received PY - 2016/02/08/revised PY - 2016/03/01/accepted PY - 2016/4/14/entrez PY - 2016/4/14/pubmed PY - 2017/11/10/medline KW - Collagen KW - Heart failure KW - Transcardiac gradient KW - miRNAs SP - 1000 EP - 8 JF - European journal of heart failure JO - Eur J Heart Fail VL - 18 IS - 8 N2 - AIMS: Differential microRNA expression in peripheral blood has been observed in patients with heart failure, suggesting their value as potential biomarkers and likely contributors to disease mechanisms. In the present study, we aimed to evaluate the transcardiac gradient of 84 cardio-microRNAs in healthy and failing hearts to determine which microRNAs are released or absorbed by the myocardium in heart failure. METHODS AND RESULTS: Eight healthy volunteers and nine patients with congestive heart failure were included. Arterial and coronary sinus blood samples were collected, and microRNAs were extracted. The expression of microRNAs was analysed using real-time PCR by the miScript miRNA PCR Array Human Cardiovascular Disease. In coronary sinus samples, the microRNAs miR-16-5p, miR-27a-3p, miR-27b-3p, miR-29b-3p, miR-29c-3p, miR-30e-5p, miR-92a-3p, miR-125b-5p, miR-140-5p, miR-195-5p, miR-424-5p, and miR-451a were significantly down-regulated, and let-7a-5p, let-7c-5p, let-7e-5p, miR-23b-3p, miR-107, miR-155-5p, miR-181a-5p, miR-181b-5p and miR-320a were up-regulated in heart failure. Left ventricular filling pressure was negatively correlated with miR-195, miR-16, miR-29b-3p, miR-29c-3p, miR-451a, and miR-92a-3p. The failing heart released let-7b-5p, let-7c-5p, let-7e-5p, miR-122-5p, and miR-21-5p, and absorbed miR-16-5p, miR-17-5p, miR-27a-3p, miR-30a-5p, miR-30d-5p, miR-30e-5p, miR-130a-3p, miR-140-5p, miR-199a-5p, and miR-451a. In silico analyses suggest that the transcardiac gradient of microRNAs in heart failure may target pathways related to heart disease. CONCLUSION: We determined the transcardiac gradient of cardio-microRNAs in failing hearts, which supports the use of these microRNAs as potential biomarkers. The microRNAs described here may have a role in the pathophysiology of heart failure as they might be involved in pathways related to disease progression, including fibrosis. SN - 1879-0844 UR - https://www.unboundmedicine.com/medline/citation/27072074/The_transcardiac_gradient_of_cardio_microRNAs_in_the_failing_heart_ DB - PRIME DP - Unbound Medicine ER -