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Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues.
J Vis Exp 2016; (109):e53886JV

Abstract

Obesity is an unconstrained worldwide epidemic. Unraveling molecular controls in adipose tissue development holds promise to treat obesity or diabetes. Although numerous immortalized adipogenic cell lines have been established, adipose-derived stem cells from the stromal vascular fraction of subcutaneous white adipose tissues provide a reliable cellular system ex vivo much closer to adipose development in vivo. Pig adipose-derived stem cells (pADSC) are isolated from 7- to 9-day old piglets. The dorsal white fat depot of porcine subcutaneous adipose tissues is sliced, minced and collagenase digested. These pADSC exhibit strong potential to differentiate into adipocytes. Moreover, the pADSC also possess multipotency, assessed by selective stem cell markers, to differentiate into various mesenchymal cell types including adipocytes, osteocytes, and chondrocytes. These pADSC can be used for clarification of molecular switches in regulating classical adipocyte differentiation or in direction to other mesenchymal cell types of mesodermal origin. Furthermore, extended lineages into cells of ectodermal and endodermal origin have recently been achieved. Therefore, pADSC derived in this protocol provide an abundant and assessable source of adult mesenchymal stem cells with full multipotency for studying adipose development and application to tissue engineering of regenerative medicine.

Authors+Show Affiliations

Institute of Biotechnology, National Taiwan University; Department of Animal Science and Technology, National Taiwan University.Department of Animal Science and Technology, National Taiwan University.Department of Animal Science and Technology, National Taiwan University.Department of Animal Science and Technology, National Taiwan University.Department of Animal Science and Technology, National Taiwan University.Department of Surgery, National Taiwan University Hospital and College of Medicine.Institute of Biotechnology, National Taiwan University; Department of Animal Science and Technology, National Taiwan University; sding@ntu.edu.tw.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Video-Audio Media

Language

eng

PubMed ID

27077225

Citation

Chen, Yu-Jen, et al. "Isolation and Differentiation of Adipose-Derived Stem Cells From Porcine Subcutaneous Adipose Tissues." Journal of Visualized Experiments : JoVE, 2016, pp. e53886.
Chen YJ, Liu HY, Chang YT, et al. Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues. J Vis Exp. 2016.
Chen, Y. J., Liu, H. Y., Chang, Y. T., Cheng, Y. H., Mersmann, H. J., Kuo, W. H., & Ding, S. T. (2016). Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues. Journal of Visualized Experiments : JoVE, (109), pp. e53886. doi:10.3791/53886.
Chen YJ, et al. Isolation and Differentiation of Adipose-Derived Stem Cells From Porcine Subcutaneous Adipose Tissues. J Vis Exp. 2016 Mar 31;(109)e53886. PubMed PMID: 27077225.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues. AU - Chen,Yu-Jen, AU - Liu,Hui-Yu, AU - Chang,Yun-Tsui, AU - Cheng,Ying-Hung, AU - Mersmann,Harry J, AU - Kuo,Wen-Hung, AU - Ding,Shih-Torng, Y1 - 2016/03/31/ PY - 2016/4/15/entrez PY - 2016/4/15/pubmed PY - 2016/12/15/medline SP - e53886 EP - e53886 JF - Journal of visualized experiments : JoVE JO - J Vis Exp IS - 109 N2 - Obesity is an unconstrained worldwide epidemic. Unraveling molecular controls in adipose tissue development holds promise to treat obesity or diabetes. Although numerous immortalized adipogenic cell lines have been established, adipose-derived stem cells from the stromal vascular fraction of subcutaneous white adipose tissues provide a reliable cellular system ex vivo much closer to adipose development in vivo. Pig adipose-derived stem cells (pADSC) are isolated from 7- to 9-day old piglets. The dorsal white fat depot of porcine subcutaneous adipose tissues is sliced, minced and collagenase digested. These pADSC exhibit strong potential to differentiate into adipocytes. Moreover, the pADSC also possess multipotency, assessed by selective stem cell markers, to differentiate into various mesenchymal cell types including adipocytes, osteocytes, and chondrocytes. These pADSC can be used for clarification of molecular switches in regulating classical adipocyte differentiation or in direction to other mesenchymal cell types of mesodermal origin. Furthermore, extended lineages into cells of ectodermal and endodermal origin have recently been achieved. Therefore, pADSC derived in this protocol provide an abundant and assessable source of adult mesenchymal stem cells with full multipotency for studying adipose development and application to tissue engineering of regenerative medicine. SN - 1940-087X UR - https://www.unboundmedicine.com/medline/citation/27077225/Isolation_and_Differentiation_of_Adipose_Derived_Stem_Cells_from_Porcine_Subcutaneous_Adipose_Tissues_ L2 - https://dx.doi.org/10.3791/53886 DB - PRIME DP - Unbound Medicine ER -