Tags

Type your tag names separated by a space and hit enter

Comparative embryotoxicity and genotoxicity of the herbicide diuron and its metabolites in early life stages of Crassostrea gigas: Implication of reactive oxygen species production.
Aquat Toxicol. 2016 Jun; 175:249-59.AT

Abstract

Herbicides are one of the major classes of pollutants contaminating coastal waters over the world. Among them, diuron (3-(3,4-dichlorophenyl)-1,1-dimethylurea) is a phenylurea herbicide frequently detected in oyster-producing area, known to be toxic for this important exploited non-target species. With the aim to investigate the mechanisms by which diuron displays its toxicity in oyster, the implication of both biotransformation and oxygen reactive species (ROS) production was studied considering embryotoxicity and genotoxicity as endpoints. Comparative embryotoxicity and genotoxicity of diuron and its main metabolites (DCPMU, DCPU and 3,4-DCA) were thus studied on oyster larvae by the embryo-larval bioassay on D larvae and the comet assay on trochophore larvae, respectively. Exposures were also performed in presence and absence of known ROS scavenger compounds - ascorbic acid and N-acetylcysteine, to evaluate the involvement of oxyradicals in the toxic responses. In the case of diuron, the production of ROS on exposed oyster larvae was also measured using 2',7'-dichlorodihydrofluorescein diacetate as a probe for flow cytometric analysis. The results we obtained showed the embryotoxicity and genotoxicity of diuron and its metabolites in early life stages of the Pacific oyster. For concentrations ranging from 0.05 to 0.5μgL(-1), diuron appeared significantly more embryotoxic than DCPMU and DCPU (p<0.001). Embryotoxicity decreased with diuron metabolism as follows: diuron≥DCPMU=DCPU, highlighting that biotransformation can constitute a true detoxication pathways in oyster larvae by decreasing the toxicity of the parent compound. In the opposite, no difference was observed between diuron and its metabolites concerning larval development when considering a lower and more environmentally realistic range of concentrations (0.002-0.050μgL(-1)). 3,4-DCA was the only compound that did not show any sign of embryotoxicity, even at concentrations up to 5μgL(-1). Concerning genotoxicity, no significant difference was observed between diuron and all of its metabolites including 3, 4 DCA with damages detected from the concentration of 0.05μgL(-1). As for diuron, the toxicity of the metabolites seems to be mediated in some part by ROS production as clearly demonstrated by the decrease in genotoxicity and developmental abnormalities in the presence of the oxidant scavenger, ascorbic acid.

Authors+Show Affiliations

Ifremer, Laboratoire d'Ecotoxicologie, Rue de l'Ile d'Yeu, BP21105, 44311 Nantes, cedex 03, France.Ifremer, Laboratoire d'Ecotoxicologie, Rue de l'Ile d'Yeu, BP21105, 44311 Nantes, cedex 03, France.Ifremer, Laboratoire d'Ecotoxicologie, Rue de l'Ile d'Yeu, BP21105, 44311 Nantes, cedex 03, France.Ifremer, Laboratoire d'Ecotoxicologie, Rue de l'Ile d'Yeu, BP21105, 44311 Nantes, cedex 03, France. Electronic address: fakcha@ifremer.fr.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27078212

Citation

Behrens, Daphné, et al. "Comparative Embryotoxicity and Genotoxicity of the Herbicide Diuron and Its Metabolites in Early Life Stages of Crassostrea Gigas: Implication of Reactive Oxygen Species Production." Aquatic Toxicology (Amsterdam, Netherlands), vol. 175, 2016, pp. 249-59.
Behrens D, Rouxel J, Burgeot T, et al. Comparative embryotoxicity and genotoxicity of the herbicide diuron and its metabolites in early life stages of Crassostrea gigas: Implication of reactive oxygen species production. Aquat Toxicol. 2016;175:249-59.
Behrens, D., Rouxel, J., Burgeot, T., & Akcha, F. (2016). Comparative embryotoxicity and genotoxicity of the herbicide diuron and its metabolites in early life stages of Crassostrea gigas: Implication of reactive oxygen species production. Aquatic Toxicology (Amsterdam, Netherlands), 175, 249-59. https://doi.org/10.1016/j.aquatox.2016.04.003
Behrens D, et al. Comparative Embryotoxicity and Genotoxicity of the Herbicide Diuron and Its Metabolites in Early Life Stages of Crassostrea Gigas: Implication of Reactive Oxygen Species Production. Aquat Toxicol. 2016;175:249-59. PubMed PMID: 27078212.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparative embryotoxicity and genotoxicity of the herbicide diuron and its metabolites in early life stages of Crassostrea gigas: Implication of reactive oxygen species production. AU - Behrens,Daphné, AU - Rouxel,Julien, AU - Burgeot,Thierry, AU - Akcha,Farida, Y1 - 2016/04/04/ PY - 2016/01/14/received PY - 2016/03/30/revised PY - 2016/04/03/accepted PY - 2016/4/15/entrez PY - 2016/4/15/pubmed PY - 2017/1/7/medline KW - Biotransformation KW - Embryotoxicity KW - Genotoxicity KW - Herbicide KW - Oxidative stress KW - Oyster SP - 249 EP - 59 JF - Aquatic toxicology (Amsterdam, Netherlands) JO - Aquat Toxicol VL - 175 N2 - Herbicides are one of the major classes of pollutants contaminating coastal waters over the world. Among them, diuron (3-(3,4-dichlorophenyl)-1,1-dimethylurea) is a phenylurea herbicide frequently detected in oyster-producing area, known to be toxic for this important exploited non-target species. With the aim to investigate the mechanisms by which diuron displays its toxicity in oyster, the implication of both biotransformation and oxygen reactive species (ROS) production was studied considering embryotoxicity and genotoxicity as endpoints. Comparative embryotoxicity and genotoxicity of diuron and its main metabolites (DCPMU, DCPU and 3,4-DCA) were thus studied on oyster larvae by the embryo-larval bioassay on D larvae and the comet assay on trochophore larvae, respectively. Exposures were also performed in presence and absence of known ROS scavenger compounds - ascorbic acid and N-acetylcysteine, to evaluate the involvement of oxyradicals in the toxic responses. In the case of diuron, the production of ROS on exposed oyster larvae was also measured using 2',7'-dichlorodihydrofluorescein diacetate as a probe for flow cytometric analysis. The results we obtained showed the embryotoxicity and genotoxicity of diuron and its metabolites in early life stages of the Pacific oyster. For concentrations ranging from 0.05 to 0.5μgL(-1), diuron appeared significantly more embryotoxic than DCPMU and DCPU (p<0.001). Embryotoxicity decreased with diuron metabolism as follows: diuron≥DCPMU=DCPU, highlighting that biotransformation can constitute a true detoxication pathways in oyster larvae by decreasing the toxicity of the parent compound. In the opposite, no difference was observed between diuron and its metabolites concerning larval development when considering a lower and more environmentally realistic range of concentrations (0.002-0.050μgL(-1)). 3,4-DCA was the only compound that did not show any sign of embryotoxicity, even at concentrations up to 5μgL(-1). Concerning genotoxicity, no significant difference was observed between diuron and all of its metabolites including 3, 4 DCA with damages detected from the concentration of 0.05μgL(-1). As for diuron, the toxicity of the metabolites seems to be mediated in some part by ROS production as clearly demonstrated by the decrease in genotoxicity and developmental abnormalities in the presence of the oxidant scavenger, ascorbic acid. SN - 1879-1514 UR - https://www.unboundmedicine.com/medline/citation/27078212/Comparative_embryotoxicity_and_genotoxicity_of_the_herbicide_diuron_and_its_metabolites_in_early_life_stages_of_Crassostrea_gigas:_Implication_of_reactive_oxygen_species_production_ DB - PRIME DP - Unbound Medicine ER -