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Plumbagin exerts protective effects in nucleus pulposus cells by attenuating hydrogen peroxide-induced oxidative stress, inflammation and apoptosis through NF-κB and Nrf-2.
Int J Mol Med. 2016 Jun; 37(6):1669-76.IJ

Abstract

Plumbagin, one of the constituents responsible for the various biological activities of Plumbago zeylanica has been demonstrated to possess antioxidant activity, which may inhibit lipid peroxidation in a dose- and time-dependent manner. In the present study, we aimed to examine the protective effects of plumbagin as well as the underlying mechansim through which plumbagin attenuates hydrogen peroxide (H2O2)-induced oxidative stress in nucleus pulposus cells (NPCs). For this purpose, the NPCs were incubated with fresh medium containing H2O2 (200 µM) at 37˚C in a humidified 5% CO2 atmosphere for 6 h, and cultured with various concentrations of plumbagin (0, 0.5, 1, 2, 5, 10 and 20 µM). Treatment with plumbagin significantly increased the viability of the H2O2-exposed NPCs in a dose‑dependent manner. Moreover, plumbagin significantly reduced the generation of reactive oxygen species, lipid peroxidation, as well as the levels of tumor necrosis factor-α (TNF-α), interleukin (IL)-1β and IL-6 in the H2O2‑exposed NPCs. Glutathione (GSH) content, as well as the activity of catalase (CAT), superoxide dismutase (SOD) and glutathione peroxdiase (GSH-Px) were increased. We found that the administration of plumbagin significantly inhibited the activity of caspase-9 and -3, and downregulated NF-κB expression and upregulated Nrf-2 expression in the H2O2-exposed NPCs. Taken together, these findings suggest that plumbagin exerts neuroprotective effects in NPCs by attenuating H2O2‑induced oxidative stress, inflammation and apoptosis through mediating the expression of NF-κB and Nrf-2.

Authors+Show Affiliations

Department of Orthopedics, The 100th Hospital of Chinese People's Liberation Army, Suzhou, Jiangsu 215007, P.R. China.Department of Orthopedics, The 100th Hospital of Chinese People's Liberation Army, Suzhou, Jiangsu 215007, P.R. China.Department of Orthopedics, The 100th Hospital of Chinese People's Liberation Army, Suzhou, Jiangsu 215007, P.R. China.Department of Orthopedics, The 100th Hospital of Chinese People's Liberation Army, Suzhou, Jiangsu 215007, P.R. China.Department of Orthopedics, The 100th Hospital of Chinese People's Liberation Army, Suzhou, Jiangsu 215007, P.R. China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27082014

Citation

Chu, Hui, et al. "Plumbagin Exerts Protective Effects in Nucleus Pulposus Cells By Attenuating Hydrogen Peroxide-induced Oxidative Stress, Inflammation and Apoptosis Through NF-κB and Nrf-2." International Journal of Molecular Medicine, vol. 37, no. 6, 2016, pp. 1669-76.
Chu H, Yu H, Ren D, et al. Plumbagin exerts protective effects in nucleus pulposus cells by attenuating hydrogen peroxide-induced oxidative stress, inflammation and apoptosis through NF-κB and Nrf-2. Int J Mol Med. 2016;37(6):1669-76.
Chu, H., Yu, H., Ren, D., Zhu, K., & Huang, H. (2016). Plumbagin exerts protective effects in nucleus pulposus cells by attenuating hydrogen peroxide-induced oxidative stress, inflammation and apoptosis through NF-κB and Nrf-2. International Journal of Molecular Medicine, 37(6), 1669-76. https://doi.org/10.3892/ijmm.2016.2564
Chu H, et al. Plumbagin Exerts Protective Effects in Nucleus Pulposus Cells By Attenuating Hydrogen Peroxide-induced Oxidative Stress, Inflammation and Apoptosis Through NF-κB and Nrf-2. Int J Mol Med. 2016;37(6):1669-76. PubMed PMID: 27082014.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Plumbagin exerts protective effects in nucleus pulposus cells by attenuating hydrogen peroxide-induced oxidative stress, inflammation and apoptosis through NF-κB and Nrf-2. AU - Chu,Hui, AU - Yu,Hang, AU - Ren,Ding, AU - Zhu,Kejun, AU - Huang,Hong, Y1 - 2016/04/14/ PY - 2015/04/20/received PY - 2016/04/01/accepted PY - 2016/4/16/entrez PY - 2016/4/16/pubmed PY - 2017/3/1/medline SP - 1669 EP - 76 JF - International journal of molecular medicine JO - Int J Mol Med VL - 37 IS - 6 N2 - Plumbagin, one of the constituents responsible for the various biological activities of Plumbago zeylanica has been demonstrated to possess antioxidant activity, which may inhibit lipid peroxidation in a dose- and time-dependent manner. In the present study, we aimed to examine the protective effects of plumbagin as well as the underlying mechansim through which plumbagin attenuates hydrogen peroxide (H2O2)-induced oxidative stress in nucleus pulposus cells (NPCs). For this purpose, the NPCs were incubated with fresh medium containing H2O2 (200 µM) at 37˚C in a humidified 5% CO2 atmosphere for 6 h, and cultured with various concentrations of plumbagin (0, 0.5, 1, 2, 5, 10 and 20 µM). Treatment with plumbagin significantly increased the viability of the H2O2-exposed NPCs in a dose‑dependent manner. Moreover, plumbagin significantly reduced the generation of reactive oxygen species, lipid peroxidation, as well as the levels of tumor necrosis factor-α (TNF-α), interleukin (IL)-1β and IL-6 in the H2O2‑exposed NPCs. Glutathione (GSH) content, as well as the activity of catalase (CAT), superoxide dismutase (SOD) and glutathione peroxdiase (GSH-Px) were increased. We found that the administration of plumbagin significantly inhibited the activity of caspase-9 and -3, and downregulated NF-κB expression and upregulated Nrf-2 expression in the H2O2-exposed NPCs. Taken together, these findings suggest that plumbagin exerts neuroprotective effects in NPCs by attenuating H2O2‑induced oxidative stress, inflammation and apoptosis through mediating the expression of NF-κB and Nrf-2. SN - 1791-244X UR - https://www.unboundmedicine.com/medline/citation/27082014/Plumbagin_exerts_protective_effects_in_nucleus_pulposus_cells_by_attenuating_hydrogen_peroxide_induced_oxidative_stress_inflammation_and_apoptosis_through_NF_κB_and_Nrf_2_ L2 - http://www.spandidos-publications.com/ijmm/37/6/1669 DB - PRIME DP - Unbound Medicine ER -