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Non-enzymatic electrochemical biosensor based on Pt NPs/RGO-CS-Fc nano-hybrids for the detection of hydrogen peroxide in living cells.
Biosens Bioelectron. 2016 Aug 15; 82:185-94.BB

Abstract

A highly sensitive non-enzymatic electrochemical sensor based on platinum nanoparticles/reduced graphene oxide-chitosan-ferrocene carboxylic acid nano-hybrids (Pt NPs/RGO-CS-Fc biosensor) was developed for the measurement of hydrogen peroxide (H2O2). The RGO-CS-Fc nano-hybrids was prepared and characterized by UV-vis spectrum, Fourier transform infrared spectroscopy, transmission electron microscopy, Raman spectrometer and electrochemical impedance spectroscopy. Under optimal experimental conditions, the Pt NPs/RGO-CS-Fc biosensor showed outstanding catalytic activity toward H2O2 reduction. The current response of the biosensor presented a linear relationship with H2O2 concentration from 2.0×10(-8)M to 3.0×10(-6)M with a correlation coefficient of R(2)=0.9968 and with logarithm of H2O2 concentration from 6.0×10(-6)M to 1.0×10(-2)M with a correlation coefficient of R(2)=0.9887, the low detection limit of 20nM was obtained at the signal/noise (S/N) ratio of 3. Moreover, the Pt NPs/RGO-CS-Fc biosensor exhibited excellent anti-interference capability and reproducibility for the detection of H2O2. The biosensor was also successfully applied for the detection of H2O2 from living cells containing normal and cancer cells. All these results prove that the Pt NPs/RGO-CS-Fc biosensor has the potential application in clinical diagnostics to evaluate oxidative stress of different living cells.

Authors+Show Affiliations

School of Life and Environment Sciences, Guilin University of Electronic Technology, Guilin, Guangxi 541004, China.School of Life and Environment Sciences, Guilin University of Electronic Technology, Guilin, Guangxi 541004, China; National Center for International Research of Biological Targeting Diagnosis and Therapy, Guangxi Key Laboratory of Biological Targeting Diagnosis and Therapy Research, Collaborative Innovation Center for Targeting Tumor Diagnosis and Therapy, Nanning, Guangxi 530021, China.School of Life and Environment Sciences, Guilin University of Electronic Technology, Guilin, Guangxi 541004, China.National Center for International Research of Biological Targeting Diagnosis and Therapy, Guangxi Key Laboratory of Biological Targeting Diagnosis and Therapy Research, Collaborative Innovation Center for Targeting Tumor Diagnosis and Therapy, Nanning, Guangxi 530021, China.Guangxi Key Laboratory of Metabolic Diseases Research, Guilin 181st Hospital, Guilin, Guangxi 541002, China.Guangxi Key Laboratory of Metabolic Diseases Research, Guilin 181st Hospital, Guilin, Guangxi 541002, China.School of Life and Environment Sciences, Guilin University of Electronic Technology, Guilin, Guangxi 541004, China; National Center for International Research of Biological Targeting Diagnosis and Therapy, Guangxi Key Laboratory of Biological Targeting Diagnosis and Therapy Research, Collaborative Innovation Center for Targeting Tumor Diagnosis and Therapy, Nanning, Guangxi 530021, China. Electronic address: huangyong503@126.com.Guangxi Key Laboratory of Metabolic Diseases Research, Guilin 181st Hospital, Guilin, Guangxi 541002, China. Electronic address: suiwg@163.com.National Center for International Research of Biological Targeting Diagnosis and Therapy, Guangxi Key Laboratory of Biological Targeting Diagnosis and Therapy Research, Collaborative Innovation Center for Targeting Tumor Diagnosis and Therapy, Nanning, Guangxi 530021, China. Electronic address: yongxiang_zhao@126.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27085950

Citation

Bai, Zhihao, et al. "Non-enzymatic Electrochemical Biosensor Based On Pt NPs/RGO-CS-Fc Nano-hybrids for the Detection of Hydrogen Peroxide in Living Cells." Biosensors & Bioelectronics, vol. 82, 2016, pp. 185-94.
Bai Z, Li G, Liang J, et al. Non-enzymatic electrochemical biosensor based on Pt NPs/RGO-CS-Fc nano-hybrids for the detection of hydrogen peroxide in living cells. Biosens Bioelectron. 2016;82:185-94.
Bai, Z., Li, G., Liang, J., Su, J., Zhang, Y., Chen, H., Huang, Y., Sui, W., & Zhao, Y. (2016). Non-enzymatic electrochemical biosensor based on Pt NPs/RGO-CS-Fc nano-hybrids for the detection of hydrogen peroxide in living cells. Biosensors & Bioelectronics, 82, 185-94. https://doi.org/10.1016/j.bios.2016.04.004
Bai Z, et al. Non-enzymatic Electrochemical Biosensor Based On Pt NPs/RGO-CS-Fc Nano-hybrids for the Detection of Hydrogen Peroxide in Living Cells. Biosens Bioelectron. 2016 Aug 15;82:185-94. PubMed PMID: 27085950.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Non-enzymatic electrochemical biosensor based on Pt NPs/RGO-CS-Fc nano-hybrids for the detection of hydrogen peroxide in living cells. AU - Bai,Zhihao, AU - Li,Guiyin, AU - Liang,Jingtao, AU - Su,Jing, AU - Zhang,Yue, AU - Chen,Huaizhou, AU - Huang,Yong, AU - Sui,Weiguo, AU - Zhao,Yongxiang, Y1 - 2016/04/08/ PY - 2016/02/21/received PY - 2016/04/02/revised PY - 2016/04/04/accepted PY - 2016/4/18/entrez PY - 2016/4/18/pubmed PY - 2017/1/24/medline KW - Ferrocene carboxylic acid KW - Graphene KW - Hydrogen peroxide KW - Non-enzymatic sensor KW - Platinum nanoparticles SP - 185 EP - 94 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 82 N2 - A highly sensitive non-enzymatic electrochemical sensor based on platinum nanoparticles/reduced graphene oxide-chitosan-ferrocene carboxylic acid nano-hybrids (Pt NPs/RGO-CS-Fc biosensor) was developed for the measurement of hydrogen peroxide (H2O2). The RGO-CS-Fc nano-hybrids was prepared and characterized by UV-vis spectrum, Fourier transform infrared spectroscopy, transmission electron microscopy, Raman spectrometer and electrochemical impedance spectroscopy. Under optimal experimental conditions, the Pt NPs/RGO-CS-Fc biosensor showed outstanding catalytic activity toward H2O2 reduction. The current response of the biosensor presented a linear relationship with H2O2 concentration from 2.0×10(-8)M to 3.0×10(-6)M with a correlation coefficient of R(2)=0.9968 and with logarithm of H2O2 concentration from 6.0×10(-6)M to 1.0×10(-2)M with a correlation coefficient of R(2)=0.9887, the low detection limit of 20nM was obtained at the signal/noise (S/N) ratio of 3. Moreover, the Pt NPs/RGO-CS-Fc biosensor exhibited excellent anti-interference capability and reproducibility for the detection of H2O2. The biosensor was also successfully applied for the detection of H2O2 from living cells containing normal and cancer cells. All these results prove that the Pt NPs/RGO-CS-Fc biosensor has the potential application in clinical diagnostics to evaluate oxidative stress of different living cells. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/27085950/Non_enzymatic_electrochemical_biosensor_based_on_Pt_NPs/RGO_CS_Fc_nano_hybrids_for_the_detection_of_hydrogen_peroxide_in_living_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(16)30278-0 DB - PRIME DP - Unbound Medicine ER -