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Pharmacological screening of Hypericum androsaemum extracts for antioxidant, anti-lipid peroxidation, antiglycation and cytotoxicity activity.
Pak J Pharm Sci. 2016 Mar; 29(2):397-405.PJ

Abstract

Oxidative stress and glycation processes have a combined effect on diabetes related complications. Crude plant extracts and plant derived compounds possessing both antiglycation and antioxidant activities have a high therapeutic potential for treating these complications. Antioxidant, antiglycation, anti-lipid per oxidation and cytotoxic activities of crude methanol extract and solvent fractions of Hypericum androsaemum L. (Hypericaceae) were evaluated and correlated with total content of phenolics and flavonoids. Significant radical scavenging activity was observed for the methanol extract against 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical used as a basis for antioxidant activity with IC50 value of 92.70±2.85 μg mL(-1) (96.20±2.34% inhibition at 500 μg mL(-1)). In case of anion scavenging activity the results were not very significant (33.20±1.22% inhibition at 500 μg mL(-1)). Anti-lipid per oxidation activity was highest for n-hexane fraction (67.83±1.33% inhibition at 500 μg mL(-1)) while the ethyl acetate fraction had the highest antiglycation activity (62.77±2.54% inhibition at 500 μg mL(-1)). Statistically significant correlation was determined for antioxidant and antiglycation activity and phenolic and flavonoid contents. In cytotoxicity assay all the extracts had IC50 values >30 μg mL(-1) as compared to the standard cycloheximide (IC50 value 0.084±0.1 μg mL(-1)). The polar extracts of H. androsaemum can be a good source of non-toxic compounds with antioxidant, anti-lipid per oxidation and antiglycation activities.

Authors+Show Affiliations

Department of Botany, Lahore College for Women University, Lahore, Pakistan.Department of Botany, Lahore College for Women University, Lahore, Pakistan.Department of Chemistry, CIIT, Abbottabad, KPK, Pakistan.Department of Chemistry, Lahore College for Women University, Lahore, Pakistan.Department of Pharmacology, University of Health Sciences, Lahore, Pakistan.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27087068

Citation

Saddiqe, Zeb, et al. "Pharmacological Screening of Hypericum Androsaemum Extracts for Antioxidant, Anti-lipid Peroxidation, Antiglycation and Cytotoxicity Activity." Pakistan Journal of Pharmaceutical Sciences, vol. 29, no. 2, 2016, pp. 397-405.
Saddiqe Z, Maimoona A, Abbas G, et al. Pharmacological screening of Hypericum androsaemum extracts for antioxidant, anti-lipid peroxidation, antiglycation and cytotoxicity activity. Pak J Pharm Sci. 2016;29(2):397-405.
Saddiqe, Z., Maimoona, A., Abbas, G., Naeem, I., & Shahzad, M. (2016). Pharmacological screening of Hypericum androsaemum extracts for antioxidant, anti-lipid peroxidation, antiglycation and cytotoxicity activity. Pakistan Journal of Pharmaceutical Sciences, 29(2), 397-405.
Saddiqe Z, et al. Pharmacological Screening of Hypericum Androsaemum Extracts for Antioxidant, Anti-lipid Peroxidation, Antiglycation and Cytotoxicity Activity. Pak J Pharm Sci. 2016;29(2):397-405. PubMed PMID: 27087068.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Pharmacological screening of Hypericum androsaemum extracts for antioxidant, anti-lipid peroxidation, antiglycation and cytotoxicity activity. AU - Saddiqe,Zeb, AU - Maimoona,Alya, AU - Abbas,Ghulam, AU - Naeem,Ismat, AU - Shahzad,Muhammad, PY - 2016/4/19/entrez PY - 2016/4/19/pubmed PY - 2016/7/1/medline SP - 397 EP - 405 JF - Pakistan journal of pharmaceutical sciences JO - Pak J Pharm Sci VL - 29 IS - 2 N2 - Oxidative stress and glycation processes have a combined effect on diabetes related complications. Crude plant extracts and plant derived compounds possessing both antiglycation and antioxidant activities have a high therapeutic potential for treating these complications. Antioxidant, antiglycation, anti-lipid per oxidation and cytotoxic activities of crude methanol extract and solvent fractions of Hypericum androsaemum L. (Hypericaceae) were evaluated and correlated with total content of phenolics and flavonoids. Significant radical scavenging activity was observed for the methanol extract against 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical used as a basis for antioxidant activity with IC50 value of 92.70±2.85 μg mL(-1) (96.20±2.34% inhibition at 500 μg mL(-1)). In case of anion scavenging activity the results were not very significant (33.20±1.22% inhibition at 500 μg mL(-1)). Anti-lipid per oxidation activity was highest for n-hexane fraction (67.83±1.33% inhibition at 500 μg mL(-1)) while the ethyl acetate fraction had the highest antiglycation activity (62.77±2.54% inhibition at 500 μg mL(-1)). Statistically significant correlation was determined for antioxidant and antiglycation activity and phenolic and flavonoid contents. In cytotoxicity assay all the extracts had IC50 values >30 μg mL(-1) as compared to the standard cycloheximide (IC50 value 0.084±0.1 μg mL(-1)). The polar extracts of H. androsaemum can be a good source of non-toxic compounds with antioxidant, anti-lipid per oxidation and antiglycation activities. SN - 1011-601X UR - https://www.unboundmedicine.com/medline/citation/27087068/Pharmacological_screening_of_Hypericum_androsaemum_extracts_for_antioxidant_anti_lipid_peroxidation_antiglycation_and_cytotoxicity_activity_ L2 - https://medlineplus.gov/antioxidants.html DB - PRIME DP - Unbound Medicine ER -