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Expression profile of PLCζ, PAWP, and TR-KIT in association with fertilization potential, embryo development, and pregnancy outcomes in globozoospermic candidates for intra-cytoplasmic sperm injection and artificial oocyte activation.
Andrology. 2016 09; 4(5):850-6.A

Abstract

Sperm-mediated oocyte activation critically depends upon appropriate expression and assembly of sperm-borne oocyte-activating factors (SOAFs) during spermiogenesis. Several factors have been considered as candidate for SOAFs over the recent years. However, little is known about the expression profile of these candidates and their potential contribution to the clinical outcomes of intra-cytoplasmic sperm injection (ICSI), particularly in globozoospermia. This study investigated the expression profile of PLCζ, PAWP, and TR-KIT and clinical outcomes of ICSI in 12 men with total globozoospermia and compared with 12 fertile individuals. Levels of PLCζ, PAWP, and TR-KIT mRNA in the spermatozoa of fertile men were significantly higher than the corresponding values of the globozoospermic subjects. Interestingly, at protein level, expressions of these factors in the cases assessed were low in globozoospermic individuals. Fertilization rates following artificial oocyte activation (AOA) in the majority of globozoospermic couples were higher than the expected 30% cut-off value reported for individuals with failed or low fertilization rate. Clinical outcomes of ICSI-AOA were dependent on inter-individual variation in globozoospermic couples. None of the SOAFs assessed could provide a greater prediction value with respect to fertilization rate in globozoospermic couples which underwent ICSI-AOA. High fertilization (56.06%) and pregnancy (41.7%) rates accomplished in this study following ICSI-AOA indicated that expression profiles of PLCζ, PAWP, and TR-KIT were low in globozoospermic individuals, and ICSI combined with artificial oocyte activation could mimic physiological calcium changes which occur during fertilization.

Authors+Show Affiliations

Department of Reproductive Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran.Department of Reproductive Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran. Isfahan Fertility and Infertility Center, Isfahan, Iran.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27089467

Citation

Tavalaee, M, and M H. Nasr-Esfahani. "Expression Profile of PLCζ, PAWP, and TR-KIT in Association With Fertilization Potential, Embryo Development, and Pregnancy Outcomes in Globozoospermic Candidates for Intra-cytoplasmic Sperm Injection and Artificial Oocyte Activation." Andrology, vol. 4, no. 5, 2016, pp. 850-6.
Tavalaee M, Nasr-Esfahani MH. Expression profile of PLCζ, PAWP, and TR-KIT in association with fertilization potential, embryo development, and pregnancy outcomes in globozoospermic candidates for intra-cytoplasmic sperm injection and artificial oocyte activation. Andrology. 2016;4(5):850-6.
Tavalaee, M., & Nasr-Esfahani, M. H. (2016). Expression profile of PLCζ, PAWP, and TR-KIT in association with fertilization potential, embryo development, and pregnancy outcomes in globozoospermic candidates for intra-cytoplasmic sperm injection and artificial oocyte activation. Andrology, 4(5), 850-6. https://doi.org/10.1111/andr.12179
Tavalaee M, Nasr-Esfahani MH. Expression Profile of PLCζ, PAWP, and TR-KIT in Association With Fertilization Potential, Embryo Development, and Pregnancy Outcomes in Globozoospermic Candidates for Intra-cytoplasmic Sperm Injection and Artificial Oocyte Activation. Andrology. 2016;4(5):850-6. PubMed PMID: 27089467.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression profile of PLCζ, PAWP, and TR-KIT in association with fertilization potential, embryo development, and pregnancy outcomes in globozoospermic candidates for intra-cytoplasmic sperm injection and artificial oocyte activation. AU - Tavalaee,M, AU - Nasr-Esfahani,M H, Y1 - 2016/04/18/ PY - 2015/09/17/received PY - 2016/02/01/revised PY - 2016/02/03/accepted PY - 2016/4/19/entrez PY - 2016/4/19/pubmed PY - 2017/11/29/medline KW - PAWP KW - PLCζ KW - TR-KIT KW - fertilization KW - globozoospermia KW - pregnancy SP - 850 EP - 6 JF - Andrology JO - Andrology VL - 4 IS - 5 N2 - Sperm-mediated oocyte activation critically depends upon appropriate expression and assembly of sperm-borne oocyte-activating factors (SOAFs) during spermiogenesis. Several factors have been considered as candidate for SOAFs over the recent years. However, little is known about the expression profile of these candidates and their potential contribution to the clinical outcomes of intra-cytoplasmic sperm injection (ICSI), particularly in globozoospermia. This study investigated the expression profile of PLCζ, PAWP, and TR-KIT and clinical outcomes of ICSI in 12 men with total globozoospermia and compared with 12 fertile individuals. Levels of PLCζ, PAWP, and TR-KIT mRNA in the spermatozoa of fertile men were significantly higher than the corresponding values of the globozoospermic subjects. Interestingly, at protein level, expressions of these factors in the cases assessed were low in globozoospermic individuals. Fertilization rates following artificial oocyte activation (AOA) in the majority of globozoospermic couples were higher than the expected 30% cut-off value reported for individuals with failed or low fertilization rate. Clinical outcomes of ICSI-AOA were dependent on inter-individual variation in globozoospermic couples. None of the SOAFs assessed could provide a greater prediction value with respect to fertilization rate in globozoospermic couples which underwent ICSI-AOA. High fertilization (56.06%) and pregnancy (41.7%) rates accomplished in this study following ICSI-AOA indicated that expression profiles of PLCζ, PAWP, and TR-KIT were low in globozoospermic individuals, and ICSI combined with artificial oocyte activation could mimic physiological calcium changes which occur during fertilization. SN - 2047-2927 UR - https://www.unboundmedicine.com/medline/citation/27089467/Expression_profile_of_PLCζ_PAWP_and_TR_KIT_in_association_with_fertilization_potential_embryo_development_and_pregnancy_outcomes_in_globozoospermic_candidates_for_intra_cytoplasmic_sperm_injection_and_artificial_oocyte_activation_ L2 - https://doi.org/10.1111/andr.12179 DB - PRIME DP - Unbound Medicine ER -