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Identification of an OmpW homologue in Burkholderia pseudomallei, a protective vaccine antigen against melioidosis.
Vaccine. 2016 05 17; 34(23):2616-21.V

Abstract

Burkholderia pseudomallei is the causative agent of melioidosis, which is associated with a range of clinical manifestations, including sepsis and fatal pneumonia and is endemic in Southeast Asia and Northern Australia. Treatment can be challenging and control of infection involves prolonged antibiotic therapy, yet there are no approved vaccines available to prevent infection. Our aim was to develop and assess the potential of a prophylactic vaccine candidate targeted against melioidosis. The identified candidate is the 22kDa outer membrane protein, OmpW. We previously demonstrated that this protein was immunoprotective in mouse models of Burkholderia cepacia complex (Bcc) infections. We cloned Bp_ompW in Escherichia coli, expressed and purified the protein. Endotoxin free protein administered with SAS adjuvant protected Balb/C mice (75% survival) relative to controls (25% survival) (p<0.05). A potent serological response was observed with IgG2a to IgG1 ratio of 6.0. Furthermore C57BL/6 mice were protected for up to 80 days against a lethal dose of B. pseudomallei and surpassed the efficacy of the live attenuated 2D2 positive control. BpompW is homologous across thirteen sequenced B. pseudomallei strains, indicating that it should be broadly protective against B. pseudomallei. In conclusion, we have demonstrated that BpOmpW is able to induce protective immunity against melioidosis and is likely to be an effective vaccine antigen, possibly in combination with other subunit antigens.

Authors+Show Affiliations

Centre of Microbial Host Interactions, ITT Dublin, Tallaght, Dublin 24, Ireland.Department of Immunology and Infection, Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, United Kingdom.Department of Immunology and Infection, Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, United Kingdom.Centre of Microbial Host Interactions, ITT Dublin, Tallaght, Dublin 24, Ireland.Institute of Biostructures and Bioimaging, National Research Council, Via Mezzocannone 16, I-80134 Naples, Italy.Institute of Biostructures and Bioimaging, National Research Council, Via Mezzocannone 16, I-80134 Naples, Italy.Department of Immunology and Infection, Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, United Kingdom.Centre of Microbial Host Interactions, ITT Dublin, Tallaght, Dublin 24, Ireland. Electronic address: Siobhan.mcclean@ittdublin.ie.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27091689

Citation

Casey, William T., et al. "Identification of an OmpW Homologue in Burkholderia Pseudomallei, a Protective Vaccine Antigen Against Melioidosis." Vaccine, vol. 34, no. 23, 2016, pp. 2616-21.
Casey WT, Spink N, Cia F, et al. Identification of an OmpW homologue in Burkholderia pseudomallei, a protective vaccine antigen against melioidosis. Vaccine. 2016;34(23):2616-21.
Casey, W. T., Spink, N., Cia, F., Collins, C., Romano, M., Berisio, R., Bancroft, G. J., & McClean, S. (2016). Identification of an OmpW homologue in Burkholderia pseudomallei, a protective vaccine antigen against melioidosis. Vaccine, 34(23), 2616-21. https://doi.org/10.1016/j.vaccine.2016.03.088
Casey WT, et al. Identification of an OmpW Homologue in Burkholderia Pseudomallei, a Protective Vaccine Antigen Against Melioidosis. Vaccine. 2016 05 17;34(23):2616-21. PubMed PMID: 27091689.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of an OmpW homologue in Burkholderia pseudomallei, a protective vaccine antigen against melioidosis. AU - Casey,William T, AU - Spink,Natasha, AU - Cia,Felipe, AU - Collins,Cassandra, AU - Romano,Maria, AU - Berisio,Rita, AU - Bancroft,Gregory J, AU - McClean,Siobhán, Y1 - 2016/04/16/ PY - 2016/01/23/received PY - 2016/03/10/revised PY - 2016/03/27/accepted PY - 2016/4/20/entrez PY - 2016/4/20/pubmed PY - 2017/10/11/medline KW - Burkholderia cepacia complex KW - Burkholderia pseudomallei KW - OmpW KW - Recombinant subunit antigen SP - 2616 EP - 21 JF - Vaccine JO - Vaccine VL - 34 IS - 23 N2 - Burkholderia pseudomallei is the causative agent of melioidosis, which is associated with a range of clinical manifestations, including sepsis and fatal pneumonia and is endemic in Southeast Asia and Northern Australia. Treatment can be challenging and control of infection involves prolonged antibiotic therapy, yet there are no approved vaccines available to prevent infection. Our aim was to develop and assess the potential of a prophylactic vaccine candidate targeted against melioidosis. The identified candidate is the 22kDa outer membrane protein, OmpW. We previously demonstrated that this protein was immunoprotective in mouse models of Burkholderia cepacia complex (Bcc) infections. We cloned Bp_ompW in Escherichia coli, expressed and purified the protein. Endotoxin free protein administered with SAS adjuvant protected Balb/C mice (75% survival) relative to controls (25% survival) (p<0.05). A potent serological response was observed with IgG2a to IgG1 ratio of 6.0. Furthermore C57BL/6 mice were protected for up to 80 days against a lethal dose of B. pseudomallei and surpassed the efficacy of the live attenuated 2D2 positive control. BpompW is homologous across thirteen sequenced B. pseudomallei strains, indicating that it should be broadly protective against B. pseudomallei. In conclusion, we have demonstrated that BpOmpW is able to induce protective immunity against melioidosis and is likely to be an effective vaccine antigen, possibly in combination with other subunit antigens. SN - 1873-2518 UR - https://www.unboundmedicine.com/medline/citation/27091689/Identification_of_an_OmpW_homologue_in_Burkholderia_pseudomallei_a_protective_vaccine_antigen_against_melioidosis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0264-410X(16)30085-8 DB - PRIME DP - Unbound Medicine ER -