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Differential effectiveness of selected non-psychotropic phytocannabinoids on human sebocyte functions implicates their introduction in dry/seborrhoeic skin and acne treatment.
Exp Dermatol 2016; 25(9):701-7ED

Abstract

Acne is a common skin disease characterized by elevated sebum production and inflammation of the sebaceous glands. We have previously shown that a non-psychotropic phytocannabinoid ((-)-cannabidiol [CBD]) exerted complex anti-acne effects by normalizing 'pro-acne agents'-induced excessive sebaceous lipid production, reducing proliferation and alleviating inflammation in human SZ95 sebocytes. Therefore, in this study we aimed to explore the putative anti-acne effects of further non-psychotropic phytocannabinoids ((-)-cannabichromene [CBC], (-)-cannabidivarin [CBDV], (-)-cannabigerol [CBG], (-)-cannabigerovarin [CBGV] and (-)-Δ(9) -tetrahydrocannabivarin [THCV]). Viability and proliferation of human SZ95 sebocytes were investigated by MTT and CyQUANT assays; cell death and lipid synthesis were monitored by DilC1 (5)-SYTOX Green labelling and Nile Red staining, respectively. Inflammatory responses were investigated by monitoring expressions of selected cytokines upon lipopolysaccharide treatment (RT-qPCR, ELISA). Up to 10 μm, the phytocannabinoids only negligibly altered the viability of the sebocytes, whereas high doses (≥50 μm) induced apoptosis. Interestingly, basal sebaceous lipid synthesis was differentially modulated by the substances: CBC and THCV suppressed it, and CBDV had only minor effects, whereas CBG and CBGV increased it. Importantly, CBC, CBDV and THCV significantly reduced arachidonic acid (AA)-induced 'acne-like' lipogenesis. Moreover, THCV suppressed proliferation, and all phytocannabinoids exerted remarkable anti-inflammatory actions. Our data suggest that CBG and CBGV may have potential in the treatment of dry-skin syndrome, whereas CBC, CBDV and especially THCV show promise to become highly efficient, novel anti-acne agents. Moreover, based on their remarkable anti-inflammatory actions, phytocannabinoids could be efficient, yet safe novel tools in the management of cutaneous inflammations.

Authors+Show Affiliations

DE-MTA 'Lendület' Cellular Physiology Research Group, Department of Physiology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.DE-MTA 'Lendület' Cellular Physiology Research Group, Department of Physiology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.DE-MTA 'Lendület' Cellular Physiology Research Group, Department of Physiology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.DE-MTA 'Lendület' Cellular Physiology Research Group, Department of Physiology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.GW Pharmaceuticals, Cambridge, UK.Departments of Dermatology, Venereology, Allergology and Immunology, Dessau Medical Center, Dessau, Germany.DE-MTA 'Lendület' Cellular Physiology Research Group, Department of Physiology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary. Department of Immunology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27094344

Citation

Oláh, Attila, et al. "Differential Effectiveness of Selected Non-psychotropic Phytocannabinoids On Human Sebocyte Functions Implicates Their Introduction in Dry/seborrhoeic Skin and Acne Treatment." Experimental Dermatology, vol. 25, no. 9, 2016, pp. 701-7.
Oláh A, Markovics A, Szabó-Papp J, et al. Differential effectiveness of selected non-psychotropic phytocannabinoids on human sebocyte functions implicates their introduction in dry/seborrhoeic skin and acne treatment. Exp Dermatol. 2016;25(9):701-7.
Oláh, A., Markovics, A., Szabó-Papp, J., Szabó, P. T., Stott, C., Zouboulis, C. C., & Bíró, T. (2016). Differential effectiveness of selected non-psychotropic phytocannabinoids on human sebocyte functions implicates their introduction in dry/seborrhoeic skin and acne treatment. Experimental Dermatology, 25(9), pp. 701-7. doi:10.1111/exd.13042.
Oláh A, et al. Differential Effectiveness of Selected Non-psychotropic Phytocannabinoids On Human Sebocyte Functions Implicates Their Introduction in Dry/seborrhoeic Skin and Acne Treatment. Exp Dermatol. 2016;25(9):701-7. PubMed PMID: 27094344.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differential effectiveness of selected non-psychotropic phytocannabinoids on human sebocyte functions implicates their introduction in dry/seborrhoeic skin and acne treatment. AU - Oláh,Attila, AU - Markovics,Arnold, AU - Szabó-Papp,Judit, AU - Szabó,Pálma Tímea, AU - Stott,Colin, AU - Zouboulis,Christos C, AU - Bíró,Tamás, Y1 - 2016/06/15/ PY - 2016/04/14/accepted PY - 2016/4/21/entrez PY - 2016/4/21/pubmed PY - 2018/1/31/medline KW - acne vulgaris KW - cutaneous inflammation KW - dry skin KW - phytocannabinoid SP - 701 EP - 7 JF - Experimental dermatology JO - Exp. Dermatol. VL - 25 IS - 9 N2 - Acne is a common skin disease characterized by elevated sebum production and inflammation of the sebaceous glands. We have previously shown that a non-psychotropic phytocannabinoid ((-)-cannabidiol [CBD]) exerted complex anti-acne effects by normalizing 'pro-acne agents'-induced excessive sebaceous lipid production, reducing proliferation and alleviating inflammation in human SZ95 sebocytes. Therefore, in this study we aimed to explore the putative anti-acne effects of further non-psychotropic phytocannabinoids ((-)-cannabichromene [CBC], (-)-cannabidivarin [CBDV], (-)-cannabigerol [CBG], (-)-cannabigerovarin [CBGV] and (-)-Δ(9) -tetrahydrocannabivarin [THCV]). Viability and proliferation of human SZ95 sebocytes were investigated by MTT and CyQUANT assays; cell death and lipid synthesis were monitored by DilC1 (5)-SYTOX Green labelling and Nile Red staining, respectively. Inflammatory responses were investigated by monitoring expressions of selected cytokines upon lipopolysaccharide treatment (RT-qPCR, ELISA). Up to 10 μm, the phytocannabinoids only negligibly altered the viability of the sebocytes, whereas high doses (≥50 μm) induced apoptosis. Interestingly, basal sebaceous lipid synthesis was differentially modulated by the substances: CBC and THCV suppressed it, and CBDV had only minor effects, whereas CBG and CBGV increased it. Importantly, CBC, CBDV and THCV significantly reduced arachidonic acid (AA)-induced 'acne-like' lipogenesis. Moreover, THCV suppressed proliferation, and all phytocannabinoids exerted remarkable anti-inflammatory actions. Our data suggest that CBG and CBGV may have potential in the treatment of dry-skin syndrome, whereas CBC, CBDV and especially THCV show promise to become highly efficient, novel anti-acne agents. Moreover, based on their remarkable anti-inflammatory actions, phytocannabinoids could be efficient, yet safe novel tools in the management of cutaneous inflammations. SN - 1600-0625 UR - https://www.unboundmedicine.com/medline/citation/27094344/Differential_effectiveness_of_selected_non_psychotropic_phytocannabinoids_on_human_sebocyte_functions_implicates_their_introduction_in_dry/seborrhoeic_skin_and_acne_treatment_ L2 - https://doi.org/10.1111/exd.13042 DB - PRIME DP - Unbound Medicine ER -