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Usefulness of a Simple Immunohistochemical Staining Technique to Differentiate Anti-p200 Pemphigoid From Other Autoimmune Blistering Diseases: A Report of 2 Cases.
Actas Dermosifiliogr. 2017 Jan - Feb; 108(1):e1-e5.AD

Abstract

Anti-p200 pemphigoid is a rare autoimmune subepidermal blistering disease characterized by the presence of circulating immunoglobulin G antibodies directed against laminin gamma-1, a 200-kDa protein located in the lamina lucida of the basement membrane. We review the clinical, histopathological and immunological characteristics of the first 2 cases described in Spain. Anti-p200 pemphigoid shares histopathological and immunopathological findings with epidermolysis bullosa acquisita, the main entity in the differential diagnosis. However, its management follows the same guidelines as those used for bullous pemphigoid. The diagnosis is confirmed by immunoblotting, which is a complex technique available in few centers. We propose the immunohistochemical detection of collagen type IV on the floor of the blister, combined with standard immunofluorescence techniques, as a simple, accessible alternative to differentiate anti-p200 pemphigoid from epidermolysis bullosa acquisita.

Authors+Show Affiliations

Departamento de Dermatología, Hospital del Mar, Parc de Salut Mar, Institut Hospital del Mar d'Investigacions Mèdiques, Barcelona, España. Electronic address: 60495@parcdesalutmar.cat.Departamento de Dermatología, Hospital del Mar, Parc de Salut Mar, Institut Hospital del Mar d'Investigacions Mèdiques, Barcelona, España.Departamento de Dermatología, Facultad de Medicina de la Universidad de Kurume, Fukuoka, Japón.Departamento de Dermatología, Facultad de Medicina de la Universidad de Kurume, Fukuoka, Japón.Departamento de Dermatología, Hospital Clínic, Barcelona, España.Departamento de Dermatología, Hospital del Mar, Parc de Salut Mar, Institut Hospital del Mar d'Investigacions Mèdiques, Barcelona, España.Departamento de Dermatología, Hospital del Mar, Parc de Salut Mar, Institut Hospital del Mar d'Investigacions Mèdiques, Barcelona, España.

Pub Type(s)

Case Reports
Journal Article

Language

eng spa

PubMed ID

27095685

Citation

García-Díez, I, et al. "Usefulness of a Simple Immunohistochemical Staining Technique to Differentiate Anti-p200 Pemphigoid From Other Autoimmune Blistering Diseases: a Report of 2 Cases." Actas Dermo-sifiliograficas, vol. 108, no. 1, 2017, pp. e1-e5.
García-Díez I, Martínez-Escala ME, Ishii N, et al. Usefulness of a Simple Immunohistochemical Staining Technique to Differentiate Anti-p200 Pemphigoid From Other Autoimmune Blistering Diseases: A Report of 2 Cases. Actas Dermosifiliogr. 2017;108(1):e1-e5.
García-Díez, I., Martínez-Escala, M. E., Ishii, N., Hashimoto, T., Mascaró Galy, J. M., Pujol, R. M., & Herrero-González, J. E. (2017). Usefulness of a Simple Immunohistochemical Staining Technique to Differentiate Anti-p200 Pemphigoid From Other Autoimmune Blistering Diseases: A Report of 2 Cases. Actas Dermo-sifiliograficas, 108(1), e1-e5. https://doi.org/10.1016/j.ad.2015.10.019
García-Díez I, et al. Usefulness of a Simple Immunohistochemical Staining Technique to Differentiate Anti-p200 Pemphigoid From Other Autoimmune Blistering Diseases: a Report of 2 Cases. Actas Dermosifiliogr. 2017 Jan - Feb;108(1):e1-e5. PubMed PMID: 27095685.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Usefulness of a Simple Immunohistochemical Staining Technique to Differentiate Anti-p200 Pemphigoid From Other Autoimmune Blistering Diseases: A Report of 2 Cases. AU - García-Díez,I, AU - Martínez-Escala,M E, AU - Ishii,N, AU - Hashimoto,T, AU - Mascaró Galy,J M, AU - Pujol,R M, AU - Herrero-González,J E, Y1 - 2016/04/16/ PY - 2015/07/14/received PY - 2015/09/17/revised PY - 2015/10/04/accepted PY - 2016/4/21/pubmed PY - 2017/11/29/medline PY - 2016/4/21/entrez KW - Anti-p200 pemphigoid KW - Bullous skin diseases KW - Collagen type IV KW - Colágeno tipo iv KW - Enfermedades ampollosas KW - Immunoblotting KW - Inmunoblot KW - Laminin gamma 1 KW - Laminina gamma-1 KW - Penfigoide anti-p200 SP - e1 EP - e5 JF - Actas dermo-sifiliograficas JO - Actas Dermosifiliogr VL - 108 IS - 1 N2 - Anti-p200 pemphigoid is a rare autoimmune subepidermal blistering disease characterized by the presence of circulating immunoglobulin G antibodies directed against laminin gamma-1, a 200-kDa protein located in the lamina lucida of the basement membrane. We review the clinical, histopathological and immunological characteristics of the first 2 cases described in Spain. Anti-p200 pemphigoid shares histopathological and immunopathological findings with epidermolysis bullosa acquisita, the main entity in the differential diagnosis. However, its management follows the same guidelines as those used for bullous pemphigoid. The diagnosis is confirmed by immunoblotting, which is a complex technique available in few centers. We propose the immunohistochemical detection of collagen type IV on the floor of the blister, combined with standard immunofluorescence techniques, as a simple, accessible alternative to differentiate anti-p200 pemphigoid from epidermolysis bullosa acquisita. SN - 1578-2190 UR - https://www.unboundmedicine.com/medline/citation/27095685/Usefulness_of_a_Simple_Immunohistochemical_Staining_Technique_to_Differentiate_Anti_p200_Pemphigoid_From_Other_Autoimmune_Blistering_Diseases:_A_Report_of_2_Cases_ L2 - https://www.elsevier.es/en/linksolver/pdf/pii/S0001-7310(16)30018-7 DB - PRIME DP - Unbound Medicine ER -