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Biochemical and histological evaluations of anti-inflammatory and antioxidant p-chloro-selenosteroid actions in acute murine models of inflammation.
Eur J Pharmacol. 2016 Jun 15; 781:25-35.EJ

Abstract

This study investigated the potential p-chloro-selenosteroid (PCS) anti-inflammatory effect in different animal models of acute inflammation. In order to determine a time- and a dose-curve response of action, female adult Swiss mice (25-35g) were divided in different groups and pretreated by the intragastric route (i.g.) with PCS (5-10mg/kg) and after the specific times (5, 30 and 60min) the ear inflammation was induced with croton oil (2.5%, 20μl). The ear edema, myeloperoxidase (MPO) activity and histological analyses were performed. In a second experiment, the pleurisy model was used to determine the PCS protective effect (10mg/kg, i.g., 30min before induction) in the inflammatory and oxidative alterations induced by an intrapleural injection of a 1% carrageenan solution (0.1ml) in exudate and lung samples. Dexamethasone (1mg/kg, i.g.) was used as positive control for both models. Statistical analysis was performed through a One-Way ANOVA test followed by the Newman-Keuls' test. Pretreatment of 30min with PCS, only at a dose of 10mg/kg, decreased ear edema and the MPO activity as well as the histological alterations induced by croton oil. In the pleurisy model, PCS (10mg/kg, i.g.; 30min) reduced the leukocyte counts, histological alterations, MPO and adenosine deaminase activities, oxidative damage and the non-enzymatic antioxidant defense imbalance. PCS had a similar anti-inflammatory profile to dexamethasone; however, it showed a better antioxidant effect. PCS had anti-inflammatory and antioxidant actions in two well established inflammation models in mice.

Authors+Show Affiliations

Departamento de Bioquímica e Biologia Molecular, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria Zip Code 97105-900, RS, Brazil.Departamento de Farmacologia, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil.Departamento de Bioquímica e Biologia Molecular, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria Zip Code 97105-900, RS, Brazil.Departamento de Bioquímica e Biologia Molecular, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria Zip Code 97105-900, RS, Brazil.Departamento de Patologia, Universidade Federal de Santa Maria, Zip Code 97105-900 RS, Brazil.Departamento de Bioquímica e Biologia Molecular, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria Zip Code 97105-900, RS, Brazil.Departamento de Bioquímica e Biologia Molecular, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria Zip Code 97105-900, RS, Brazil. Electronic address: criswn@ufsm.br.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27102337

Citation

Marcondes Sari, Marcel Henrique, et al. "Biochemical and Histological Evaluations of Anti-inflammatory and Antioxidant P-chloro-selenosteroid Actions in Acute Murine Models of Inflammation." European Journal of Pharmacology, vol. 781, 2016, pp. 25-35.
Marcondes Sari MH, Souza AC, Rosa SG, et al. Biochemical and histological evaluations of anti-inflammatory and antioxidant p-chloro-selenosteroid actions in acute murine models of inflammation. Eur J Pharmacol. 2016;781:25-35.
Marcondes Sari, M. H., Souza, A. C., Rosa, S. G., Chagas, P. M., da Luz, S. C., Rodrigues, O. E., & Nogueira, C. W. (2016). Biochemical and histological evaluations of anti-inflammatory and antioxidant p-chloro-selenosteroid actions in acute murine models of inflammation. European Journal of Pharmacology, 781, 25-35. https://doi.org/10.1016/j.ejphar.2016.03.051
Marcondes Sari MH, et al. Biochemical and Histological Evaluations of Anti-inflammatory and Antioxidant P-chloro-selenosteroid Actions in Acute Murine Models of Inflammation. Eur J Pharmacol. 2016 Jun 15;781:25-35. PubMed PMID: 27102337.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Biochemical and histological evaluations of anti-inflammatory and antioxidant p-chloro-selenosteroid actions in acute murine models of inflammation. AU - Marcondes Sari,Marcel Henrique, AU - Souza,Ana Cristina Guerra, AU - Rosa,Suzan Gonçalves, AU - Chagas,Pietro Maria, AU - da Luz,Sônia Cristina Almeida, AU - Rodrigues,Oscar Endrigo Dorneles, AU - Nogueira,Cristina Wayne, Y1 - 2016/04/18/ PY - 2015/09/09/received PY - 2016/03/23/revised PY - 2016/03/29/accepted PY - 2016/4/23/entrez PY - 2016/4/23/pubmed PY - 2017/3/9/medline KW - Carrageenan KW - Croton oil KW - Inflammation KW - Mice KW - Organoselenium KW - Selenium SP - 25 EP - 35 JF - European journal of pharmacology JO - Eur J Pharmacol VL - 781 N2 - This study investigated the potential p-chloro-selenosteroid (PCS) anti-inflammatory effect in different animal models of acute inflammation. In order to determine a time- and a dose-curve response of action, female adult Swiss mice (25-35g) were divided in different groups and pretreated by the intragastric route (i.g.) with PCS (5-10mg/kg) and after the specific times (5, 30 and 60min) the ear inflammation was induced with croton oil (2.5%, 20μl). The ear edema, myeloperoxidase (MPO) activity and histological analyses were performed. In a second experiment, the pleurisy model was used to determine the PCS protective effect (10mg/kg, i.g., 30min before induction) in the inflammatory and oxidative alterations induced by an intrapleural injection of a 1% carrageenan solution (0.1ml) in exudate and lung samples. Dexamethasone (1mg/kg, i.g.) was used as positive control for both models. Statistical analysis was performed through a One-Way ANOVA test followed by the Newman-Keuls' test. Pretreatment of 30min with PCS, only at a dose of 10mg/kg, decreased ear edema and the MPO activity as well as the histological alterations induced by croton oil. In the pleurisy model, PCS (10mg/kg, i.g.; 30min) reduced the leukocyte counts, histological alterations, MPO and adenosine deaminase activities, oxidative damage and the non-enzymatic antioxidant defense imbalance. PCS had a similar anti-inflammatory profile to dexamethasone; however, it showed a better antioxidant effect. PCS had anti-inflammatory and antioxidant actions in two well established inflammation models in mice. SN - 1879-0712 UR - https://www.unboundmedicine.com/medline/citation/27102337/Biochemical_and_histological_evaluations_of_anti_inflammatory_and_antioxidant_p_chloro_selenosteroid_actions_in_acute_murine_models_of_inflammation_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-2999(16)30190-X DB - PRIME DP - Unbound Medicine ER -