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Investigation on hypoglycemic effects of ethanol extract of Alpinia nigra (Gaertn.) in animal model.
J Intercult Ethnopharmacol 2016 Mar-Apr; 5(2):131-6JI

Abstract

BACKGROUND

Our study aims at exploring the hypoglycemic effect, efficacy, and possible mode of action of ethanol extract of Alpinia nigra (EEAN) as an antidiabetic agent in an animal model.

METHODS

Oral glucose tolerance test (OGTT) was used to identify primary hypoglycemic effect in mice. Three tests (glucose absorption, sucrose absorption, and disaccharidase activity) were carried out by gut perfusion and six segments studies to assess carbohydrate absorption and glucose utilization.

RESULTS

In OGTT, at 400 mg/kg and 800 mg/kg dose of EEAN extract significantly improved oral glucose tolerance among normal mice at 60 min and 90 min with compared to control. Both doses of extract significantly (P < 0.01) reduced blood glucose level and showed the hypoglycemic effect by retarding 11.43% and 20.82% of blood glucose level after 2 h of administration in glucose-induced mice, respectively. In situ perfused rat intestinal model demonstrated reduced glucose absorption at a 500 mg/kg dose. Inhibition of intestinal disaccharidase was also found by the extract. This was confirmed, yet again, via the six segment study. Throughout the length of the gastrointestinal tract, sucrose digestion was found to be inhibited which is also evident in the six segment study.

CONCLUSIONS

This study suggests that the EEAN has hypoglycemic effects in a dose-dependent manner by inhibiting intestinal glucose absorption, and these may be effective in the treatment of diabetes. Further study is required to explicate the effect this extract or the active compounds have on the individual glucose transporters and the precise mechanism.

Authors+Show Affiliations

Department of Pharmacy, International Islamic University Chittagong, Chittagong-4203, Bangladesh.Department of Pharmacy, University of Chittagong, Chittagong - 4331, Bangladesh.Department of Pharmacology, Division of Pharmacology Research, Bangladesh Council of Scientific and Industrial Research, Chittagong - 4220, Bangladesh.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27104033

Citation

Kabir, Mohammad Shah Hafez, et al. "Investigation On Hypoglycemic Effects of Ethanol Extract of Alpinia Nigra (Gaertn.) in Animal Model." Journal of Intercultural Ethnopharmacology, vol. 5, no. 2, 2016, pp. 131-6.
Kabir MS, Uddin MM, Hosen SM. Investigation on hypoglycemic effects of ethanol extract of Alpinia nigra (Gaertn.) in animal model. J Intercult Ethnopharmacol. 2016;5(2):131-6.
Kabir, M. S., Uddin, M. M., & Hosen, S. M. (2016). Investigation on hypoglycemic effects of ethanol extract of Alpinia nigra (Gaertn.) in animal model. Journal of Intercultural Ethnopharmacology, 5(2), pp. 131-6. doi:10.5455/jice.20160307112256.
Kabir MS, Uddin MM, Hosen SM. Investigation On Hypoglycemic Effects of Ethanol Extract of Alpinia Nigra (Gaertn.) in Animal Model. J Intercult Ethnopharmacol. 2016;5(2):131-6. PubMed PMID: 27104033.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Investigation on hypoglycemic effects of ethanol extract of Alpinia nigra (Gaertn.) in animal model. AU - Kabir,Mohammad Shah Hafez, AU - Uddin,Mir Muhammad Nasir, AU - Hosen,S M Zahid, Y1 - 2016/03/24/ PY - 2015/12/22/received PY - 2016/02/24/accepted PY - 2016/4/23/entrez PY - 2016/4/23/pubmed PY - 2016/4/23/medline KW - Alpinia nigra KW - blood glucose KW - glucose tolerance KW - hypoglycemic effect KW - oral glucose tolerance test SP - 131 EP - 6 JF - Journal of intercultural ethnopharmacology JO - J Intercult Ethnopharmacol VL - 5 IS - 2 N2 - BACKGROUND: Our study aims at exploring the hypoglycemic effect, efficacy, and possible mode of action of ethanol extract of Alpinia nigra (EEAN) as an antidiabetic agent in an animal model. METHODS: Oral glucose tolerance test (OGTT) was used to identify primary hypoglycemic effect in mice. Three tests (glucose absorption, sucrose absorption, and disaccharidase activity) were carried out by gut perfusion and six segments studies to assess carbohydrate absorption and glucose utilization. RESULTS: In OGTT, at 400 mg/kg and 800 mg/kg dose of EEAN extract significantly improved oral glucose tolerance among normal mice at 60 min and 90 min with compared to control. Both doses of extract significantly (P < 0.01) reduced blood glucose level and showed the hypoglycemic effect by retarding 11.43% and 20.82% of blood glucose level after 2 h of administration in glucose-induced mice, respectively. In situ perfused rat intestinal model demonstrated reduced glucose absorption at a 500 mg/kg dose. Inhibition of intestinal disaccharidase was also found by the extract. This was confirmed, yet again, via the six segment study. Throughout the length of the gastrointestinal tract, sucrose digestion was found to be inhibited which is also evident in the six segment study. CONCLUSIONS: This study suggests that the EEAN has hypoglycemic effects in a dose-dependent manner by inhibiting intestinal glucose absorption, and these may be effective in the treatment of diabetes. Further study is required to explicate the effect this extract or the active compounds have on the individual glucose transporters and the precise mechanism. SN - 2146-8397 UR - https://www.unboundmedicine.com/medline/citation/27104033/Investigation_on_hypoglycemic_effects_of_ethanol_extract_of_Alpinia_nigra__Gaertn___in_animal_model_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/27104033/ DB - PRIME DP - Unbound Medicine ER -