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Overexpression of sucrose transporter gene PbSUT2 from Pyrus bretschneideri, enhances sucrose content in Solanum lycopersicum fruit.
Plant Physiol Biochem. 2016 Aug; 105:150-161.PP

Abstract

Sucrose transporters (SUTs) belong to the major facilitator superfamily. The function of SUTs has been intensively investigated in some higher plants, whereas that in pear fruit is unknown. In this study, the cloning and functional characterization of a sucrose transporter, PbSUT2, in pear (Pyrus bretschneideri Rehd. cv. 'Yali') fruits are reported. PbSUT2 encoded a protein of 498 amino acid residues, and was localized in the plasma membrane of transformed onion epidermal cells and Arabidopsis protoplasts. Phylogenetic analysis revealed that PbSUT2 belonged to the SUT4 clade. The phenotype of overexpression of PbSUT2 tomato plants included early flowering, higher fruit quantity and lower plant height. Overexpression of PbSUT2 in transgenic tomato plants led to increases in the net photosynthetic rate in leaves and sucrose content in mature fruit compared with wild-type tomato plants, and a decrease in the contents of glucose, fructose and total soluble sugars in mature fruits. These results suggested that PbSUT2 affected sucrose content in sinks and the flowering phase during tomato plant growth and development.

Authors+Show Affiliations

Center of Pear Engineering & Technology Research, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China; Gold Mantis School of Architecture, Soochow University, Suzhou, 215123, China.Center of Pear Engineering & Technology Research, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China.Center of Pear Engineering & Technology Research, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China.Center of Pear Engineering & Technology Research, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China.Center of Pear Engineering & Technology Research, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China.Center of Pear Engineering & Technology Research, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China.Center of Pear Engineering & Technology Research, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China. Electronic address: slzhang@njau.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27105422

Citation

Wang, Li-Fen, et al. "Overexpression of Sucrose Transporter Gene PbSUT2 From Pyrus Bretschneideri, Enhances Sucrose Content in Solanum Lycopersicum Fruit." Plant Physiology and Biochemistry : PPB, vol. 105, 2016, pp. 150-161.
Wang LF, Qi XX, Huang XS, et al. Overexpression of sucrose transporter gene PbSUT2 from Pyrus bretschneideri, enhances sucrose content in Solanum lycopersicum fruit. Plant Physiol Biochem. 2016;105:150-161.
Wang, L. F., Qi, X. X., Huang, X. S., Xu, L. L., Jin, C., Wu, J., & Zhang, S. L. (2016). Overexpression of sucrose transporter gene PbSUT2 from Pyrus bretschneideri, enhances sucrose content in Solanum lycopersicum fruit. Plant Physiology and Biochemistry : PPB, 105, 150-161. https://doi.org/10.1016/j.plaphy.2016.04.019
Wang LF, et al. Overexpression of Sucrose Transporter Gene PbSUT2 From Pyrus Bretschneideri, Enhances Sucrose Content in Solanum Lycopersicum Fruit. Plant Physiol Biochem. 2016;105:150-161. PubMed PMID: 27105422.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Overexpression of sucrose transporter gene PbSUT2 from Pyrus bretschneideri, enhances sucrose content in Solanum lycopersicum fruit. AU - Wang,Li-Fen, AU - Qi,Xiao-Xiao, AU - Huang,Xiao-San, AU - Xu,Lin-Lin, AU - Jin,Cong, AU - Wu,Jun, AU - Zhang,Shao-Ling, Y1 - 2016/04/13/ PY - 2015/12/31/received PY - 2016/04/10/revised PY - 2016/04/10/accepted PY - 2016/4/23/entrez PY - 2016/4/23/pubmed PY - 2017/3/21/medline KW - Flowering KW - Pear KW - Photosynthetic rate KW - Sucrose transporter KW - Sugar content KW - Transgenic tomato SP - 150 EP - 161 JF - Plant physiology and biochemistry : PPB JO - Plant Physiol Biochem VL - 105 N2 - Sucrose transporters (SUTs) belong to the major facilitator superfamily. The function of SUTs has been intensively investigated in some higher plants, whereas that in pear fruit is unknown. In this study, the cloning and functional characterization of a sucrose transporter, PbSUT2, in pear (Pyrus bretschneideri Rehd. cv. 'Yali') fruits are reported. PbSUT2 encoded a protein of 498 amino acid residues, and was localized in the plasma membrane of transformed onion epidermal cells and Arabidopsis protoplasts. Phylogenetic analysis revealed that PbSUT2 belonged to the SUT4 clade. The phenotype of overexpression of PbSUT2 tomato plants included early flowering, higher fruit quantity and lower plant height. Overexpression of PbSUT2 in transgenic tomato plants led to increases in the net photosynthetic rate in leaves and sucrose content in mature fruit compared with wild-type tomato plants, and a decrease in the contents of glucose, fructose and total soluble sugars in mature fruits. These results suggested that PbSUT2 affected sucrose content in sinks and the flowering phase during tomato plant growth and development. SN - 1873-2690 UR - https://www.unboundmedicine.com/medline/citation/27105422/Overexpression_of_sucrose_transporter_gene_PbSUT2_from_Pyrus_bretschneideri_enhances_sucrose_content_in_Solanum_lycopersicum_fruit_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0981-9428(16)30134-6 DB - PRIME DP - Unbound Medicine ER -