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Molecular Profiling of Shiga Toxin-Producing Escherichia coli and Enteropathogenic E. coli Strains Isolated from French Coastal Environments.
Appl Environ Microbiol. 2016 07 01; 82(13):3913-3927.AE

Abstract

Shiga toxin-producing Escherichia coli (STEC) and enteropathogenic E. coli (EPEC) strains may be responsible for food-borne infections in humans. Twenty-eight STEC and 75 EPEC strains previously isolated from French shellfish-harvesting areas and their watersheds and belonging to 68 distinguishable serotypes were characterized in this study. High-throughput real-time PCR was used to search for the presence of 75 E. coli virulence-associated gene targets, and genes encoding Shiga toxin (stx) and intimin (eae) were subtyped using PCR tests and DNA sequencing, respectively. The results showed a high level of diversity between strains, with 17 unique virulence gene profiles for STEC and 56 for EPEC. Seven STEC and 15 EPEC strains were found to display a large number or a particular combination of genetic markers of virulence and the presence of stx and/or eae variants, suggesting their potential pathogenicity for humans. Among these, an O26:H11 stx1a eae-β1 strain was associated with a large number of virulence-associated genes (n = 47), including genes carried on the locus of enterocyte effacement (LEE) or other pathogenicity islands, such as OI-122, OI-71, OI-43/48, OI-50, OI-57, and the high-pathogenicity island (HPI). One O91:H21 STEC strain containing 4 stx variants (stx1a, stx2a, stx2c, and stx2d) was found to possess genes associated with pathogenicity islands OI-122, OI-43/48, and OI-15. Among EPEC strains harboring a large number of virulence genes (n, 34 to 50), eight belonged to serotype O26:H11, O103:H2, O103:H25, O145:H28, O157:H7, or O153:H2.

IMPORTANCE

The species E. coli includes a wide variety of strains, some of which may be responsible for severe infections. This study, a molecular risk assessment study of E. coli strains isolated from the coastal environment, was conducted to evaluate the potential risk for shellfish consumers. This report describes the characterization of virulence gene profiles and stx/eae polymorphisms of E. coli isolates and clearly highlights the finding that the majority of strains isolated from coastal environment are potentially weakly pathogenic, while some are likely to be more pathogenic.

Authors+Show Affiliations

IFREMER, Département Ressources Biologiques et Environnement, Unité Santé, Génétique et Microbiologie des Mollusques, Laboratoire Santé Environnement et Microbiologie, Plouzané, France.U2RM EA4655 Stress/Virulence, Normandie-Université (UCBN), Caen, France.Université Paris-Est, ANSES Food Safety Laboratory, Platform IdentyPath, Maisons-Alfort, France.Université Paris-Est, ANSES Food Safety Laboratory, Platform IdentyPath, Maisons-Alfort, France.IFREMER, Département Ressources Biologiques et Environnement, Unité Santé, Génétique et Microbiologie des Mollusques, Laboratoire Santé Environnement et Microbiologie, Plouzané, France Michele.Gourmelon@ifremer.fr.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27107119

Citation

Balière, C, et al. "Molecular Profiling of Shiga Toxin-Producing Escherichia Coli and Enteropathogenic E. Coli Strains Isolated From French Coastal Environments." Applied and Environmental Microbiology, vol. 82, no. 13, 2016, pp. 3913-3927.
Balière C, Rincé A, Delannoy S, et al. Molecular Profiling of Shiga Toxin-Producing Escherichia coli and Enteropathogenic E. coli Strains Isolated from French Coastal Environments. Appl Environ Microbiol. 2016;82(13):3913-3927.
Balière, C., Rincé, A., Delannoy, S., Fach, P., & Gourmelon, M. (2016). Molecular Profiling of Shiga Toxin-Producing Escherichia coli and Enteropathogenic E. coli Strains Isolated from French Coastal Environments. Applied and Environmental Microbiology, 82(13), 3913-3927. https://doi.org/10.1128/AEM.00271-16
Balière C, et al. Molecular Profiling of Shiga Toxin-Producing Escherichia Coli and Enteropathogenic E. Coli Strains Isolated From French Coastal Environments. Appl Environ Microbiol. 2016 07 1;82(13):3913-3927. PubMed PMID: 27107119.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular Profiling of Shiga Toxin-Producing Escherichia coli and Enteropathogenic E. coli Strains Isolated from French Coastal Environments. AU - Balière,C, AU - Rincé,A, AU - Delannoy,S, AU - Fach,P, AU - Gourmelon,M, Y1 - 2016/06/13/ PY - 2016/01/26/received PY - 2016/04/17/accepted PY - 2016/4/24/entrez PY - 2016/4/24/pubmed PY - 2017/10/11/medline SP - 3913 EP - 3927 JF - Applied and environmental microbiology JO - Appl Environ Microbiol VL - 82 IS - 13 N2 - UNLABELLED: Shiga toxin-producing Escherichia coli (STEC) and enteropathogenic E. coli (EPEC) strains may be responsible for food-borne infections in humans. Twenty-eight STEC and 75 EPEC strains previously isolated from French shellfish-harvesting areas and their watersheds and belonging to 68 distinguishable serotypes were characterized in this study. High-throughput real-time PCR was used to search for the presence of 75 E. coli virulence-associated gene targets, and genes encoding Shiga toxin (stx) and intimin (eae) were subtyped using PCR tests and DNA sequencing, respectively. The results showed a high level of diversity between strains, with 17 unique virulence gene profiles for STEC and 56 for EPEC. Seven STEC and 15 EPEC strains were found to display a large number or a particular combination of genetic markers of virulence and the presence of stx and/or eae variants, suggesting their potential pathogenicity for humans. Among these, an O26:H11 stx1a eae-β1 strain was associated with a large number of virulence-associated genes (n = 47), including genes carried on the locus of enterocyte effacement (LEE) or other pathogenicity islands, such as OI-122, OI-71, OI-43/48, OI-50, OI-57, and the high-pathogenicity island (HPI). One O91:H21 STEC strain containing 4 stx variants (stx1a, stx2a, stx2c, and stx2d) was found to possess genes associated with pathogenicity islands OI-122, OI-43/48, and OI-15. Among EPEC strains harboring a large number of virulence genes (n, 34 to 50), eight belonged to serotype O26:H11, O103:H2, O103:H25, O145:H28, O157:H7, or O153:H2. IMPORTANCE: The species E. coli includes a wide variety of strains, some of which may be responsible for severe infections. This study, a molecular risk assessment study of E. coli strains isolated from the coastal environment, was conducted to evaluate the potential risk for shellfish consumers. This report describes the characterization of virulence gene profiles and stx/eae polymorphisms of E. coli isolates and clearly highlights the finding that the majority of strains isolated from coastal environment are potentially weakly pathogenic, while some are likely to be more pathogenic. SN - 1098-5336 UR - https://www.unboundmedicine.com/medline/citation/27107119/Molecular_Profiling_of_Shiga_Toxin_Producing_Escherichia_coli_and_Enteropathogenic_E__coli_Strains_Isolated_from_French_Coastal_Environments_ L2 - http://aem.asm.org/cgi/pmidlookup?view=long&pmid=27107119 DB - PRIME DP - Unbound Medicine ER -