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Phylogeny of Geminivirus Coat Protein Sequences and Digital PCR Aid in Identifying Spissistilus festinus as a Vector of Grapevine red blotch-associated virus.
Phytopathology. 2016 10; 106(10):1223-1230.P

Abstract

Grapevine red blotch-associated virus (GRBaV) is a single-stranded DNA virus, proposed to be a member of the family Geminiviridae, and is associated with grapevines showing red blotch symptoms in North America. The existence of the virus was reported in 2012, and subsequently detected in grapevines in major grape production regions. We investigated if a vector exists that can transmit GRBaV in vineyards. Phylogenetic analysis of the predicted amino acid sequence of coat protein (CP) of GRBaV with the CP of 23 geminiviruses representing all seven genera of the family Geminiviridae revealed that GRBaV-CP was most similar to that of Tomato pseudo-curly top virus, a geminivirus known to be transmitted by a treehopper (Membracidae), a family that is closely related to leafhoppers (Cicadellidae). To identify vectors of GRBaV, hemipteran species within and nearby wine grape vineyards where virus spread was suspected were collected and transmission assays were conducted. Among the species tested, the three-cornered alfalfa hopper Spissistilus festinus (Hemiptera: Membracidae) was able to both acquire the virus from a grapevine infected with GRBaV and transmit the virus to healthy grapevines in the laboratory. In commercial vineyards, lateral shoots of grapevines girdled due to feeding injury by the adult three-cornered alfalfa hopper also tested positive for the virus using digital PCR. These findings represent an important step in understanding the biology of GRBaV and develop management guidelines.

Authors+Show Affiliations

First, third, and fourth authors: U.S. Department of Agriculture-Agriculture Research Services, Department of Plant Pathology, University of California, One Shields Ave., Davis 95616; and first and second authors: Department of Entomology and Nematology, University of California, One Shields Ave., Davis 95616.First, third, and fourth authors: U.S. Department of Agriculture-Agriculture Research Services, Department of Plant Pathology, University of California, One Shields Ave., Davis 95616; and first and second authors: Department of Entomology and Nematology, University of California, One Shields Ave., Davis 95616.First, third, and fourth authors: U.S. Department of Agriculture-Agriculture Research Services, Department of Plant Pathology, University of California, One Shields Ave., Davis 95616; and first and second authors: Department of Entomology and Nematology, University of California, One Shields Ave., Davis 95616.First, third, and fourth authors: U.S. Department of Agriculture-Agriculture Research Services, Department of Plant Pathology, University of California, One Shields Ave., Davis 95616; and first and second authors: Department of Entomology and Nematology, University of California, One Shields Ave., Davis 95616.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27111804

Citation

Bahder, Brian W., et al. "Phylogeny of Geminivirus Coat Protein Sequences and Digital PCR Aid in Identifying Spissistilus Festinus as a Vector of Grapevine Red Blotch-associated Virus." Phytopathology, vol. 106, no. 10, 2016, pp. 1223-1230.
Bahder BW, Zalom FG, Jayanth M, et al. Phylogeny of Geminivirus Coat Protein Sequences and Digital PCR Aid in Identifying Spissistilus festinus as a Vector of Grapevine red blotch-associated virus. Phytopathology. 2016;106(10):1223-1230.
Bahder, B. W., Zalom, F. G., Jayanth, M., & Sudarshana, M. R. (2016). Phylogeny of Geminivirus Coat Protein Sequences and Digital PCR Aid in Identifying Spissistilus festinus as a Vector of Grapevine red blotch-associated virus. Phytopathology, 106(10), 1223-1230.
Bahder BW, et al. Phylogeny of Geminivirus Coat Protein Sequences and Digital PCR Aid in Identifying Spissistilus Festinus as a Vector of Grapevine Red Blotch-associated Virus. Phytopathology. 2016;106(10):1223-1230. PubMed PMID: 27111804.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phylogeny of Geminivirus Coat Protein Sequences and Digital PCR Aid in Identifying Spissistilus festinus as a Vector of Grapevine red blotch-associated virus. AU - Bahder,Brian W, AU - Zalom,Frank G, AU - Jayanth,Maya, AU - Sudarshana,Mysore R, Y1 - 2016/07/27/ PY - 2016/4/26/entrez PY - 2016/4/26/pubmed PY - 2017/7/1/medline KW - E. variabilis KW - E. ziczac KW - Erythroneura elegantula SP - 1223 EP - 1230 JF - Phytopathology JO - Phytopathology VL - 106 IS - 10 N2 - Grapevine red blotch-associated virus (GRBaV) is a single-stranded DNA virus, proposed to be a member of the family Geminiviridae, and is associated with grapevines showing red blotch symptoms in North America. The existence of the virus was reported in 2012, and subsequently detected in grapevines in major grape production regions. We investigated if a vector exists that can transmit GRBaV in vineyards. Phylogenetic analysis of the predicted amino acid sequence of coat protein (CP) of GRBaV with the CP of 23 geminiviruses representing all seven genera of the family Geminiviridae revealed that GRBaV-CP was most similar to that of Tomato pseudo-curly top virus, a geminivirus known to be transmitted by a treehopper (Membracidae), a family that is closely related to leafhoppers (Cicadellidae). To identify vectors of GRBaV, hemipteran species within and nearby wine grape vineyards where virus spread was suspected were collected and transmission assays were conducted. Among the species tested, the three-cornered alfalfa hopper Spissistilus festinus (Hemiptera: Membracidae) was able to both acquire the virus from a grapevine infected with GRBaV and transmit the virus to healthy grapevines in the laboratory. In commercial vineyards, lateral shoots of grapevines girdled due to feeding injury by the adult three-cornered alfalfa hopper also tested positive for the virus using digital PCR. These findings represent an important step in understanding the biology of GRBaV and develop management guidelines. SN - 0031-949X UR - https://www.unboundmedicine.com/medline/citation/27111804/Phylogeny_of_Geminivirus_Coat_Protein_Sequences_and_Digital_PCR_Aid_in_Identifying_Spissistilus_festinus_as_a_Vector_of_Grapevine_red_blotch_associated_virus_ DB - PRIME DP - Unbound Medicine ER -