Tags

Type your tag names separated by a space and hit enter

Long-term effects of abscisic acid (ABA) on the grape berry phenylpropanoid pathway: Gene expression and metabolite content.
Plant Physiol Biochem. 2016 Aug; 105:213-223.PP

Abstract

ABA has been proposed as the main signal triggering the onset of the ripening process in grapes, and modulating the secondary metabolism in grape berry skins. To determine the effect of ABA on secondary metabolism in berries, clusters of Carménère were sprayed with 0 μLL(-1) ABA; 50 μLL(-1) ABA and 100 μLL(-1) ABA during pre-véraison, and the gene expression of the transcription factors and enzymes of the phenylpropanoid pathway were assessed from véraison to 70 days after véraison (DAV). Additionally, flavonols, tannins and anthocyanins were assessed from véraison until harvest (110 DAV). ABA accelerated sugar and anthocyanin accumulation at véraison. The grape transcript abundance of VvDFR, VvANS, VvUFGT and VvMybA1, all peaking around véraison mimicked the concentration of ABA throughout the season. The highest anthocyanin concentration occurred 35 DAV for all treatments, but higher pigment concentrations were observed in ABA-treated berries at véraison and from 60 to 70 DAV to harvest. VvPAL was also increased by treatment at the higher concentration of ABA from véraison to 40 DAV. Regarding flavanol synthesis, VvLAR2 and VvMyb4A decreased from véraison until 40 DAV and then increased again until 70 DAV. Compared to the control, both ABA treatments resulted in a less-than-proportional reduction of the expression of both genes compared to the control and, after 40 DAV, in a more-than-proportional increase compared to the control, suggesting a long-term effect of the pre-véraison ABA spray on the berries. A concomitant increase in flavanols was observed in berries after 40 DAV, and this occurred at a higher extent in berries treated with the highest ABA concentration.

Authors+Show Affiliations

Facultad de Ciencias Agronómicas, Universidad de Chile, Casilla, 1004, Santiago, Chile.Facultad de Ciencias Agronómicas, Universidad de Chile, Casilla, 1004, Santiago, Chile.Facultad de Ciencias Agronómicas, Universidad de Chile, Casilla, 1004, Santiago, Chile.Facultad de Ciencias Agronómicas, Universidad de Chile, Casilla, 1004, Santiago, Chile. Electronic address: cpastene@uchile.cl.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27116369

Citation

Villalobos-González, Luis, et al. "Long-term Effects of Abscisic Acid (ABA) On the Grape Berry Phenylpropanoid Pathway: Gene Expression and Metabolite Content." Plant Physiology and Biochemistry : PPB, vol. 105, 2016, pp. 213-223.
Villalobos-González L, Peña-Neira A, Ibáñez F, et al. Long-term effects of abscisic acid (ABA) on the grape berry phenylpropanoid pathway: Gene expression and metabolite content. Plant Physiol Biochem. 2016;105:213-223.
Villalobos-González, L., Peña-Neira, A., Ibáñez, F., & Pastenes, C. (2016). Long-term effects of abscisic acid (ABA) on the grape berry phenylpropanoid pathway: Gene expression and metabolite content. Plant Physiology and Biochemistry : PPB, 105, 213-223. https://doi.org/10.1016/j.plaphy.2016.04.012
Villalobos-González L, et al. Long-term Effects of Abscisic Acid (ABA) On the Grape Berry Phenylpropanoid Pathway: Gene Expression and Metabolite Content. Plant Physiol Biochem. 2016;105:213-223. PubMed PMID: 27116369.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Long-term effects of abscisic acid (ABA) on the grape berry phenylpropanoid pathway: Gene expression and metabolite content. AU - Villalobos-González,Luis, AU - Peña-Neira,Alvaro, AU - Ibáñez,Freddy, AU - Pastenes,Claudio, Y1 - 2016/04/19/ PY - 2016/01/30/received PY - 2016/04/05/revised PY - 2016/04/07/accepted PY - 2016/4/27/entrez PY - 2016/4/27/pubmed PY - 2017/3/21/medline KW - Abscisic acid KW - Grape berry KW - Phenylpropanoids SP - 213 EP - 223 JF - Plant physiology and biochemistry : PPB JO - Plant Physiol Biochem VL - 105 N2 - ABA has been proposed as the main signal triggering the onset of the ripening process in grapes, and modulating the secondary metabolism in grape berry skins. To determine the effect of ABA on secondary metabolism in berries, clusters of Carménère were sprayed with 0 μLL(-1) ABA; 50 μLL(-1) ABA and 100 μLL(-1) ABA during pre-véraison, and the gene expression of the transcription factors and enzymes of the phenylpropanoid pathway were assessed from véraison to 70 days after véraison (DAV). Additionally, flavonols, tannins and anthocyanins were assessed from véraison until harvest (110 DAV). ABA accelerated sugar and anthocyanin accumulation at véraison. The grape transcript abundance of VvDFR, VvANS, VvUFGT and VvMybA1, all peaking around véraison mimicked the concentration of ABA throughout the season. The highest anthocyanin concentration occurred 35 DAV for all treatments, but higher pigment concentrations were observed in ABA-treated berries at véraison and from 60 to 70 DAV to harvest. VvPAL was also increased by treatment at the higher concentration of ABA from véraison to 40 DAV. Regarding flavanol synthesis, VvLAR2 and VvMyb4A decreased from véraison until 40 DAV and then increased again until 70 DAV. Compared to the control, both ABA treatments resulted in a less-than-proportional reduction of the expression of both genes compared to the control and, after 40 DAV, in a more-than-proportional increase compared to the control, suggesting a long-term effect of the pre-véraison ABA spray on the berries. A concomitant increase in flavanols was observed in berries after 40 DAV, and this occurred at a higher extent in berries treated with the highest ABA concentration. SN - 1873-2690 UR - https://www.unboundmedicine.com/medline/citation/27116369/Long_term_effects_of_abscisic_acid__ABA__on_the_grape_berry_phenylpropanoid_pathway:_Gene_expression_and_metabolite_content_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0981-9428(16)30127-9 DB - PRIME DP - Unbound Medicine ER -