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Illumina-based analysis of the rhizosphere microbial communities associated with healthy and wilted Lanzhou lily (Lilium davidii var. unicolor) plants grown in the field.
World J Microbiol Biotechnol. 2016 Jun; 32(6):95.WJ

Abstract

Lanzhou lily (Liliumdavidii var. unicolor) is the best edible lily as well as a traditional medicinal plant in China. The microbes associated with plant roots play crucial roles in plant growth and health. However, little is known about the differences of rhizosphere microbes between healthy and wilted Lanzhou lily (Lilium davidii var. unicolor) plants. The objective of this study was to compare the rhizosphere microbial community and functional diversity of healthy and wilted plants, and to identify potential biocontrol agents with significant effect. Paired end Illumina Mi-Seq sequencing of 16S rRNA and ITS gene amplicons was employed to study the bacterial and fungal communities in the rhizosphere soil of Lanzhou lily plants. BIOLOG technology was adopted to investigate the microbial functional diversity. Our results indicated that there were major differences in the rhizosphere microbial composition and functional diversity of wilted samples compared with healthy samples. Healthy Lanzhou lily plants exhibited lower rhizosphere-associated bacterial diversity than diseased plants, whereas fungi exhibited the opposite trend. The dominant phyla in both the healthy and wilted samples were Proteobacteria and Ascomycota, i.e., 34.45 and 64.01 %, respectively. The microbial functional diversity was suppressed in wilted soil samples. Besides Fusarium, the higher relative abundances of Rhizoctonia, Verticillium, Penicillium, and Ilyonectria (Neonectria) in the wilted samples suggest they may pathogenetic root rot fungi. The high relative abundances of Bacillus in Firmicutes in healthy samples may have significant roles as biological control agents against soilborne pathogens. This is the first study to find evidence of major differences between the microbial communities in the rhizospheric soil of healthy and wilted Lanzhou lily, which may be linked to the health status of plants.

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Authors+Show Affiliations

Shang Q
Key Laboratory of Stress Physiology and Ecology, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou, 730000, China. University of Chinese Academy of Sciences, Beijing, 100049, China.
Yang G
Key Laboratory of Stress Physiology and Ecology, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou, 730000, China.
Wang Y
Key Laboratory of Desert and Desertification, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou, 730000, China.
Wu X
Key Laboratory of Desert and Desertification, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou, 730000, China.
Zhao X
Key Laboratory of Stress Physiology and Ecology, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou, 730000, China. University of Chinese Academy of Sciences, Beijing, 100049, China.
Hao H
Key Laboratory of Stress Physiology and Ecology, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou, 730000, China. University of Chinese Academy of Sciences, Beijing, 100049, China.
Li Y
School of Life Sciences, Lanzhou University, Lanzhou, 730000, China.
Xie Z
Key Laboratory of Stress Physiology and Ecology, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou, 730000, China.
Zhang Y
Key Laboratory of Stress Physiology and Ecology, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou, 730000, China.
Wang R
Key Laboratory of Stress Physiology and Ecology, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou, 730000, China. wangruoyu@lzb.ac.cn.

MeSH

BacteriaBiodiversityChinaFungiLiliumMicrobiotaPlant DiseasesPlant RootsRNA, Ribosomal, 16SRhizosphereSoilSoil Microbiology

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27116961

Citation

Shang, Qianhan, et al. "Illumina-based Analysis of the Rhizosphere Microbial Communities Associated With Healthy and Wilted Lanzhou Lily (Lilium Davidii Var. Unicolor) Plants Grown in the Field." World Journal of Microbiology & Biotechnology, vol. 32, no. 6, 2016, p. 95.
Shang Q, Yang G, Wang Y, et al. Illumina-based analysis of the rhizosphere microbial communities associated with healthy and wilted Lanzhou lily (Lilium davidii var. unicolor) plants grown in the field. World J Microbiol Biotechnol. 2016;32(6):95.
Shang, Q., Yang, G., Wang, Y., Wu, X., Zhao, X., Hao, H., Li, Y., Xie, Z., Zhang, Y., & Wang, R. (2016). Illumina-based analysis of the rhizosphere microbial communities associated with healthy and wilted Lanzhou lily (Lilium davidii var. unicolor) plants grown in the field. World Journal of Microbiology & Biotechnology, 32(6), 95. https://doi.org/10.1007/s11274-016-2051-2
Shang Q, et al. Illumina-based Analysis of the Rhizosphere Microbial Communities Associated With Healthy and Wilted Lanzhou Lily (Lilium Davidii Var. Unicolor) Plants Grown in the Field. World J Microbiol Biotechnol. 2016;32(6):95. PubMed PMID: 27116961.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Illumina-based analysis of the rhizosphere microbial communities associated with healthy and wilted Lanzhou lily (Lilium davidii var. unicolor) plants grown in the field. AU - Shang,Qianhan, AU - Yang,Guo, AU - Wang,Yun, AU - Wu,Xiukun, AU - Zhao,Xia, AU - Hao,Haiting, AU - Li,Yuyao, AU - Xie,Zhongkui, AU - Zhang,Yubao, AU - Wang,Ruoyu, Y1 - 2016/04/27/ PY - 2015/10/30/received PY - 2016/03/12/accepted PY - 2016/4/28/entrez PY - 2016/4/28/pubmed PY - 2017/4/14/medline KW - Bacillus KW - Biolog KW - Fusarium KW - Lanzhou lily KW - Microbial diversity KW - Wilt SP - 95 EP - 95 JF - World journal of microbiology & biotechnology JO - World J Microbiol Biotechnol VL - 32 IS - 6 N2 - Lanzhou lily (Liliumdavidii var. unicolor) is the best edible lily as well as a traditional medicinal plant in China. The microbes associated with plant roots play crucial roles in plant growth and health. However, little is known about the differences of rhizosphere microbes between healthy and wilted Lanzhou lily (Lilium davidii var. unicolor) plants. The objective of this study was to compare the rhizosphere microbial community and functional diversity of healthy and wilted plants, and to identify potential biocontrol agents with significant effect. Paired end Illumina Mi-Seq sequencing of 16S rRNA and ITS gene amplicons was employed to study the bacterial and fungal communities in the rhizosphere soil of Lanzhou lily plants. BIOLOG technology was adopted to investigate the microbial functional diversity. Our results indicated that there were major differences in the rhizosphere microbial composition and functional diversity of wilted samples compared with healthy samples. Healthy Lanzhou lily plants exhibited lower rhizosphere-associated bacterial diversity than diseased plants, whereas fungi exhibited the opposite trend. The dominant phyla in both the healthy and wilted samples were Proteobacteria and Ascomycota, i.e., 34.45 and 64.01 %, respectively. The microbial functional diversity was suppressed in wilted soil samples. Besides Fusarium, the higher relative abundances of Rhizoctonia, Verticillium, Penicillium, and Ilyonectria (Neonectria) in the wilted samples suggest they may pathogenetic root rot fungi. The high relative abundances of Bacillus in Firmicutes in healthy samples may have significant roles as biological control agents against soilborne pathogens. This is the first study to find evidence of major differences between the microbial communities in the rhizospheric soil of healthy and wilted Lanzhou lily, which may be linked to the health status of plants. SN - 1573-0972 UR - https://www.unboundmedicine.com/medline/citation/27116961/Illumina_based_analysis_of_the_rhizosphere_microbial_communities_associated_with_healthy_and_wilted_Lanzhou_lily__Lilium_davidii_var__unicolor__plants_grown_in_the_field_ DB - PRIME DP - Unbound Medicine ER -