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Gadolinium and ruthenium red attenuate remote hind limb preconditioning-induced cardioprotection: possible role of TRP and especially TRPV channels.
Naunyn Schmiedebergs Arch Pharmacol. 2016 Aug; 389(8):887-96.NS

Abstract

Remote ischemic preconditioning is a well reported therapeutic strategy that induces cardioprotective effects but the underlying intracellular mechanisms have not been widely explored. The current study was designed to investigate the involvement of TRP and especially TRPV channels in remote hind limb preconditioning-induced cardioprotection. Remote hind limb preconditioning stimulus (4 alternate cycles of inflation and deflation of 5 min each) was delivered using a blood pressure cuff tied on the hind limb of the anesthetized rat. Using Langendorff's system, the heart was perfused and subjected to 30-min ischemia and 120-min reperfusion. The myocardial injury was assessed by measuring infarct size, lactate dehydrogenase (LDH), creatine kinase (CK), LVDP, +dp/dtmax, -dp/dtmin, heart rate, and coronary flow rate. Gadolinium, TRP blocker, and ruthenium red, TRPV channel blocker, were employed as pharmacological tools. Remote hind limb preconditioning significantly reduced the infarct size, LDH release, CK release and improved coronary flow rate, hemodynamic parameters including LVDP, +dp/dtmax, -dp/dtmin, and heart rate. However, gadolinium (7.5 and 15 mg kg(-1)) and ruthenium red (4 and 8 mg kg(-1)) significantly attenuated the cardioprotective effects suggesting the involvement of TRP especially TRPV channels in mediating remote hind limb preconditioning-induced cardioprotection. Remote hind limb preconditioning stimulus possibly activates TRPV channels on the heart or sensory nerve fibers innervating the heart to induce cardioprotective effects. Alternatively, remote hind limb preconditioning stimulus may also activate the mechanosensitive TRP and especially TRPV channels on the sensory nerve fibers innervating the skeletal muscles to trigger cardioprotective neurogenic signaling cascade. The cardioprotective effects of remote hind limb preconditioning may be mediated via activation of mechanosensitive TRP and especially TRPV channels.

Authors+Show Affiliations

Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala, 147002, India.Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala, 147002, India. amteshwarjaggi@yahoo.co.in.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27118661

Citation

Randhawa, Puneet Kaur, and Amteshwar Singh Jaggi. "Gadolinium and Ruthenium Red Attenuate Remote Hind Limb Preconditioning-induced Cardioprotection: Possible Role of TRP and Especially TRPV Channels." Naunyn-Schmiedeberg's Archives of Pharmacology, vol. 389, no. 8, 2016, pp. 887-96.
Randhawa PK, Jaggi AS. Gadolinium and ruthenium red attenuate remote hind limb preconditioning-induced cardioprotection: possible role of TRP and especially TRPV channels. Naunyn Schmiedebergs Arch Pharmacol. 2016;389(8):887-96.
Randhawa, P. K., & Jaggi, A. S. (2016). Gadolinium and ruthenium red attenuate remote hind limb preconditioning-induced cardioprotection: possible role of TRP and especially TRPV channels. Naunyn-Schmiedeberg's Archives of Pharmacology, 389(8), 887-96. https://doi.org/10.1007/s00210-016-1251-5
Randhawa PK, Jaggi AS. Gadolinium and Ruthenium Red Attenuate Remote Hind Limb Preconditioning-induced Cardioprotection: Possible Role of TRP and Especially TRPV Channels. Naunyn Schmiedebergs Arch Pharmacol. 2016;389(8):887-96. PubMed PMID: 27118661.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Gadolinium and ruthenium red attenuate remote hind limb preconditioning-induced cardioprotection: possible role of TRP and especially TRPV channels. AU - Randhawa,Puneet Kaur, AU - Jaggi,Amteshwar Singh, Y1 - 2016/04/27/ PY - 2015/12/09/received PY - 2016/04/20/accepted PY - 2016/4/28/entrez PY - 2016/4/28/pubmed PY - 2017/2/22/medline KW - Heart KW - Mechanosensitive channels KW - Remote hind limb preconditioning KW - TRPV channels SP - 887 EP - 96 JF - Naunyn-Schmiedeberg's archives of pharmacology JO - Naunyn Schmiedebergs Arch Pharmacol VL - 389 IS - 8 N2 - Remote ischemic preconditioning is a well reported therapeutic strategy that induces cardioprotective effects but the underlying intracellular mechanisms have not been widely explored. The current study was designed to investigate the involvement of TRP and especially TRPV channels in remote hind limb preconditioning-induced cardioprotection. Remote hind limb preconditioning stimulus (4 alternate cycles of inflation and deflation of 5 min each) was delivered using a blood pressure cuff tied on the hind limb of the anesthetized rat. Using Langendorff's system, the heart was perfused and subjected to 30-min ischemia and 120-min reperfusion. The myocardial injury was assessed by measuring infarct size, lactate dehydrogenase (LDH), creatine kinase (CK), LVDP, +dp/dtmax, -dp/dtmin, heart rate, and coronary flow rate. Gadolinium, TRP blocker, and ruthenium red, TRPV channel blocker, were employed as pharmacological tools. Remote hind limb preconditioning significantly reduced the infarct size, LDH release, CK release and improved coronary flow rate, hemodynamic parameters including LVDP, +dp/dtmax, -dp/dtmin, and heart rate. However, gadolinium (7.5 and 15 mg kg(-1)) and ruthenium red (4 and 8 mg kg(-1)) significantly attenuated the cardioprotective effects suggesting the involvement of TRP especially TRPV channels in mediating remote hind limb preconditioning-induced cardioprotection. Remote hind limb preconditioning stimulus possibly activates TRPV channels on the heart or sensory nerve fibers innervating the heart to induce cardioprotective effects. Alternatively, remote hind limb preconditioning stimulus may also activate the mechanosensitive TRP and especially TRPV channels on the sensory nerve fibers innervating the skeletal muscles to trigger cardioprotective neurogenic signaling cascade. The cardioprotective effects of remote hind limb preconditioning may be mediated via activation of mechanosensitive TRP and especially TRPV channels. SN - 1432-1912 UR - https://www.unboundmedicine.com/medline/citation/27118661/Gadolinium_and_ruthenium_red_attenuate_remote_hind_limb_preconditioning_induced_cardioprotection:_possible_role_of_TRP_and_especially_TRPV_channels_ L2 - https://dx.doi.org/10.1007/s00210-016-1251-5 DB - PRIME DP - Unbound Medicine ER -