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Sesquiterpene lactone containing extracts from two cultivars of forage chicory (Cichorium intybus) show distinctive chemical profiles and in vitro activity against Ostertagia ostertagi.
Int J Parasitol Drugs Drug Resist. 2015 12; 5(3):191-200.IJ

Abstract

The study investigated direct anthelmintic effects of sesquiterpene lactones (SL)-containing extracts from forage chicory against free-living and parasitic stages of Ostertagia ostertagi. Freeze-dried leaves from chicory cultivars 'Spadona' and 'Puna II' were extracted using methanol/water. Total SL were further fractionated by solid-phase extraction and resulting extracts were characterised by high-performance liquid chromatography (HPLC). O. ostertagi eggs from faeces of mono-infected calves were hatched and L1 were used in a larval feeding inhibition assay (LFIA), while cultured L3 were used in a larval exsheathment inhibition assay (LEIA). Adult worms were immediately recovered after slaughter and used for motility inhibition assays (AMIA). Electron microscopy (EM) was performed on adult O. ostertagi exposed to 1000 μg extract mL(-1) of both chicory cultivars. In all assays, decreasing concentrations of SL-containing extracts in PBS (1% DMSO) were tested in replicates with 1% DMSO in PBS as negative controls. HPLC demonstrated similar concentrations of most SL in both extracts. However, Spadona-extract contained significantly higher concentrations of 11, 13-dihydro-8-deoxylactucin (P = 0.01), while Puna II-extract had increased levels of 11, 13-dihydrolactucin (P < 0.0001). In the LFIA, both extracts reduced larval feeding at increasing concentrations, but Spadona-extract showed higher potency confirmed by significantly lower EC50 (P < 0.0001). In the LEIA, neither of the two extracts interfered with the exsheathment of L3 (P > 0.05). In the AMIA, both SL-containing extracts induced a dose-dependent effect but Spadona-extract showed greater activity and exerted faster worm paralysis than Puna II-extract with significantly lower EC50 (P < 0.0001). No cuticular damage was observed by EM in worms exposed to any of the extracts. We have demonstrated that SL-containing extracts from forage chicory can inhibit feeding of free-living larvae and exert direct effects against parasitic stages of O. ostertagi. Our results may contribute to the identification of natural anti-parasitic compounds and to interpret the in vivo anthelmintic effects of forage chicory.

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Authors+Show Affiliations

Peña-Espinoza M
Section for Bacteriology, Pathology and Parasitology, National Veterinary Institute, Technical University of Denmark, Bülowsvej 27, 1870 Frederiksberg C, Denmark. Electronic address: miap@vet.dtu.dk.
Boas U
Section for Immunology and Vaccinology, National Veterinary Institute, Technical University of Denmark, Bülowsvej 27, DK, 1870 Frederiksberg C, Denmark.
Williams AR
Section for Parasitology and Aquatic Diseases, Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Dyrlægevej 100, 1870 Frederiksberg C, Denmark.
Thamsborg SM
Section for Parasitology and Aquatic Diseases, Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Dyrlægevej 100, 1870 Frederiksberg C, Denmark.
Simonsen HT
Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Thorvaldsensvej 40, 1871 Frederiksberg C, Denmark.
Enemark HL
Section for Bacteriology, Pathology and Parasitology, National Veterinary Institute, Technical University of Denmark, Bülowsvej 27, 1870 Frederiksberg C, Denmark.

MeSH

AnimalsAnthelminticsChicoryLactonesOstertagiaPlant ExtractsSesquiterpenes

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27120066

Citation

Peña-Espinoza, Miguel, et al. "Sesquiterpene Lactone Containing Extracts From Two Cultivars of Forage Chicory (Cichorium Intybus) Show Distinctive Chemical Profiles and in Vitro Activity Against Ostertagia Ostertagi." International Journal for Parasitology. Drugs and Drug Resistance, vol. 5, no. 3, 2015, pp. 191-200.
Peña-Espinoza M, Boas U, Williams AR, et al. Sesquiterpene lactone containing extracts from two cultivars of forage chicory (Cichorium intybus) show distinctive chemical profiles and in vitro activity against Ostertagia ostertagi. Int J Parasitol Drugs Drug Resist. 2015;5(3):191-200.
Peña-Espinoza, M., Boas, U., Williams, A. R., Thamsborg, S. M., Simonsen, H. T., & Enemark, H. L. (2015). Sesquiterpene lactone containing extracts from two cultivars of forage chicory (Cichorium intybus) show distinctive chemical profiles and in vitro activity against Ostertagia ostertagi. International Journal for Parasitology. Drugs and Drug Resistance, 5(3), 191-200. https://doi.org/10.1016/j.ijpddr.2015.10.002
Peña-Espinoza M, et al. Sesquiterpene Lactone Containing Extracts From Two Cultivars of Forage Chicory (Cichorium Intybus) Show Distinctive Chemical Profiles and in Vitro Activity Against Ostertagia Ostertagi. Int J Parasitol Drugs Drug Resist. 2015;5(3):191-200. PubMed PMID: 27120066.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sesquiterpene lactone containing extracts from two cultivars of forage chicory (Cichorium intybus) show distinctive chemical profiles and in vitro activity against Ostertagia ostertagi. AU - Peña-Espinoza,Miguel, AU - Boas,Ulrik, AU - Williams,Andrew R, AU - Thamsborg,Stig M, AU - Simonsen,Henrik T, AU - Enemark,Heidi L, Y1 - 2015/10/22/ PY - 2015/08/10/received PY - 2015/10/08/revised PY - 2015/10/08/accepted PY - 2016/4/28/entrez PY - 2016/4/28/pubmed PY - 2016/4/28/medline KW - Anthelmintic KW - Cattle KW - Electron microscopy KW - Forage chicory KW - Ostertagia ostertagi KW - Sesquiterpene lactones SP - 191 EP - 200 JF - International journal for parasitology. Drugs and drug resistance JO - Int J Parasitol Drugs Drug Resist VL - 5 IS - 3 N2 - The study investigated direct anthelmintic effects of sesquiterpene lactones (SL)-containing extracts from forage chicory against free-living and parasitic stages of Ostertagia ostertagi. Freeze-dried leaves from chicory cultivars 'Spadona' and 'Puna II' were extracted using methanol/water. Total SL were further fractionated by solid-phase extraction and resulting extracts were characterised by high-performance liquid chromatography (HPLC). O. ostertagi eggs from faeces of mono-infected calves were hatched and L1 were used in a larval feeding inhibition assay (LFIA), while cultured L3 were used in a larval exsheathment inhibition assay (LEIA). Adult worms were immediately recovered after slaughter and used for motility inhibition assays (AMIA). Electron microscopy (EM) was performed on adult O. ostertagi exposed to 1000 μg extract mL(-1) of both chicory cultivars. In all assays, decreasing concentrations of SL-containing extracts in PBS (1% DMSO) were tested in replicates with 1% DMSO in PBS as negative controls. HPLC demonstrated similar concentrations of most SL in both extracts. However, Spadona-extract contained significantly higher concentrations of 11, 13-dihydro-8-deoxylactucin (P = 0.01), while Puna II-extract had increased levels of 11, 13-dihydrolactucin (P < 0.0001). In the LFIA, both extracts reduced larval feeding at increasing concentrations, but Spadona-extract showed higher potency confirmed by significantly lower EC50 (P < 0.0001). In the LEIA, neither of the two extracts interfered with the exsheathment of L3 (P > 0.05). In the AMIA, both SL-containing extracts induced a dose-dependent effect but Spadona-extract showed greater activity and exerted faster worm paralysis than Puna II-extract with significantly lower EC50 (P < 0.0001). No cuticular damage was observed by EM in worms exposed to any of the extracts. We have demonstrated that SL-containing extracts from forage chicory can inhibit feeding of free-living larvae and exert direct effects against parasitic stages of O. ostertagi. Our results may contribute to the identification of natural anti-parasitic compounds and to interpret the in vivo anthelmintic effects of forage chicory. SN - 2211-3207 UR - https://www.unboundmedicine.com/medline/citation/27120066/Sesquiterpene_lactone_containing_extracts_from_two_cultivars_of_forage_chicory__Cichorium_intybus__show_distinctive_chemical_profiles_and_in_vitro_activity_against_Ostertagia_ostertagi_ DB - PRIME DP - Unbound Medicine ER -