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Antibacterial effect of N-acetylcysteine on common canine otitis externa isolates.
Vet Dermatol. 2016 Jun; 27(3):188-e47.VD

Abstract

BACKGROUND

N-Acetylcysteine (NAC) has the potential to be a useful therapeutic agent for the treatment of otitis externa due to its antimicrobial and mucolytic properties, as well as its ability to disrupt bacterial biofilm.

HYPOTHESIS/OBJECTIVES

To determine the antibacterial activity of NAC against common bacterial isolates associated with canine otitis externa.

ANIMALS

Twenty two isolates from canine clinical cases of otitis externa were identified and tested, including five Staphylococcus pseudintermedius, six Pseudomonas aeruginosa, five Corynebacterium spp. and six β-haemolytic Streptococcus spp. isolates.

METHODS

Each isolate was grown on blood agar for 24 h and transferred to Mueller Hinton Broth (MHB) to achieve a final concentration of 5 × 10(5) CFU/mL. NAC was diluted in MHB to a starting concentration of 160 mg/mL and serial two-fold microdilution assays were performed in triplicate with negative controls for all isolates tested. Concentrations of NAC tested ranged from 0.125 to 80 mg/mL. A 50 μL volume of bacterial suspension was used to inoculate each well.

RESULTS

The minimum inhibitory concentration (MIC) of NAC for all isolates tested ranged from 5 to 20 mg/mL.

CONCLUSIONS AND CLINICAL RELEVANCE

N-Acetylcysteine inhibits clinically relevant and drug resistant bacteria in vitro, and has potential for use as a novel agent for treatment of otitis externa.

Authors+Show Affiliations

Departments of Small Animal Clinical Sciences, University of Tennessee, 2407 River Drive, Knoxville, TN, 37996, USA.Departments of Small Animal Clinical Sciences, University of Tennessee, 2407 River Drive, Knoxville, TN, 37996, USA.Biomedical and Diagnostic Sciences, University of Tennessee, 2407 River Drive, Knoxville, TN, 37996, USA.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27122224

Citation

May, Elizabeth R., et al. "Antibacterial Effect of N-acetylcysteine On Common Canine Otitis Externa Isolates." Veterinary Dermatology, vol. 27, no. 3, 2016, pp. 188-e47.
May ER, Conklin KA, Bemis DA. Antibacterial effect of N-acetylcysteine on common canine otitis externa isolates. Vet Dermatol. 2016;27(3):188-e47.
May, E. R., Conklin, K. A., & Bemis, D. A. (2016). Antibacterial effect of N-acetylcysteine on common canine otitis externa isolates. Veterinary Dermatology, 27(3), 188-e47. https://doi.org/10.1111/vde.12313
May ER, Conklin KA, Bemis DA. Antibacterial Effect of N-acetylcysteine On Common Canine Otitis Externa Isolates. Vet Dermatol. 2016;27(3):188-e47. PubMed PMID: 27122224.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antibacterial effect of N-acetylcysteine on common canine otitis externa isolates. AU - May,Elizabeth R, AU - Conklin,Katherine A, AU - Bemis,David A, Y1 - 2016/04/28/ PY - 2016/03/11/accepted PY - 2016/4/29/entrez PY - 2016/4/29/pubmed PY - 2017/4/19/medline SP - 188 EP - e47 JF - Veterinary dermatology JO - Vet. Dermatol. VL - 27 IS - 3 N2 - BACKGROUND: N-Acetylcysteine (NAC) has the potential to be a useful therapeutic agent for the treatment of otitis externa due to its antimicrobial and mucolytic properties, as well as its ability to disrupt bacterial biofilm. HYPOTHESIS/OBJECTIVES: To determine the antibacterial activity of NAC against common bacterial isolates associated with canine otitis externa. ANIMALS: Twenty two isolates from canine clinical cases of otitis externa were identified and tested, including five Staphylococcus pseudintermedius, six Pseudomonas aeruginosa, five Corynebacterium spp. and six β-haemolytic Streptococcus spp. isolates. METHODS: Each isolate was grown on blood agar for 24 h and transferred to Mueller Hinton Broth (MHB) to achieve a final concentration of 5 × 10(5) CFU/mL. NAC was diluted in MHB to a starting concentration of 160 mg/mL and serial two-fold microdilution assays were performed in triplicate with negative controls for all isolates tested. Concentrations of NAC tested ranged from 0.125 to 80 mg/mL. A 50 μL volume of bacterial suspension was used to inoculate each well. RESULTS: The minimum inhibitory concentration (MIC) of NAC for all isolates tested ranged from 5 to 20 mg/mL. CONCLUSIONS AND CLINICAL RELEVANCE: N-Acetylcysteine inhibits clinically relevant and drug resistant bacteria in vitro, and has potential for use as a novel agent for treatment of otitis externa. SN - 1365-3164 UR - https://www.unboundmedicine.com/medline/citation/27122224/Antibacterial_effect_of_N_acetylcysteine_on_common_canine_otitis_externa_isolates_ L2 - https://doi.org/10.1111/vde.12313 DB - PRIME DP - Unbound Medicine ER -