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Interleukin-4 enzyme-linked immunospot assay may be useful for diagnosing sensitization to house dust mite.
Int Forum Allergy Rhinol. 2016 10; 6(10):1007-1012.IF

Abstract

BACKGROUND

The skin prick test (SPT) is considered a standard test for identification of allergens, but it has some limitations in clinical practice. The multiple allergen simultaneous test (MAST), which measures allergen-specific immunoglobulin E in patients' serum, is a widely used alternative test, but is limited by its relatively low sensitivity and specificity. As a novel diagnostic test to identify allergens, we investigated the sensitivity and specificity of an interleukin-4 (IL-4) enzyme-linked immunospot (ELISpot) assay for Dermatophagoides farinae (Der f) and Dermatophagoides pteronyssinus (Der p).

METHODS

Based on the symptoms and SPT results, 43 house dust mite (HDM) allergic rhinitis (AR) patients and 41 control subjects were included. Peripheral blood was drawn from each subject for IL-4 ELISpot assay and MAST. The receiver operating characteristic (ROC) curve analysis was conducted to determine the cutoff values. Sensitivity, specificity, and positive and neg predictive values were compared between the 2 tests.

RESULTS

The sensitivity, specificity, and areas under the ROC curve (AUCs) of the IL-4 ELISpot assay were 88.4%, 97.6%, and 0.939 for Der f, and 95.3%, 97.5%, and 0.971 for Der p, respectively. However, the sensitivity, specificity, and AUC of MAST were 76.7%, 73.2%, and 0.777 for Der f, and 69.8%, 75.6%, and 0.788 for Der p, respectively.

CONCLUSION

The IL-4 ELISpot assay showed higher sensitivity, specificity, and AUC than MAST, which indicates its clinical feasibility for diagnosing allergy for HDM. A further study is needed to determine the accuracy of the IL-4 ELISpot assay for other common allergens.

Authors+Show Affiliations

Institute of Health Sciences, Gyeongsang National University, Jinju, Republic of Korea. Department of Otorhinolaryngology, Gyeongsang National University Hospital, Jinju, Republic of Korea.Laboratory of Immunology and Infectious Diseases, Graduate School of Medical Science and Engineering, KAIST, Daejeon, Republic of Korea.Laboratory of Immunology and Infectious Diseases, Graduate School of Medical Science and Engineering, KAIST, Daejeon, Republic of Korea.Department of Otorhinolaryngology, Gyeongsang National University Hospital, Jinju, Republic of Korea.Department of Otorhinolaryngology, Gyeongsang National University Hospital, Jinju, Republic of Korea.Department of Otorhinolaryngology, Gyeongsang National University Hospital, Jinju, Republic of Korea.Department of Otorhinolaryngology, Gyeongsang National University Hospital, Jinju, Republic of Korea.Institute of Health Sciences, Gyeongsang National University, Jinju, Republic of Korea. Department of Otorhinolaryngology, Gyeongsang National University Hospital, Jinju, Republic of Korea.Institute of Health Sciences, Gyeongsang National University, Jinju, Republic of Korea. Department of Otorhinolaryngology, Gyeongsang National University Hospital, Jinju, Republic of Korea. Department of Otorhinolaryngology, Gyeongsang National University School of Medicine, Jinju, Republic of Korea.Regional Cardiocerebrovascular Disease Center, Gyeongsang National University Hospital, Jinju, Republic of Korea.Institute of Health Sciences, Gyeongsang National University, Jinju, Republic of Korea. astroflower77@hanmail.net. Department of Otorhinolaryngology, Gyeongsang National University Hospital, Jinju, Republic of Korea. astroflower77@hanmail.net. Department of Otorhinolaryngology, Gyeongsang National University School of Medicine, Jinju, Republic of Korea. astroflower77@hanmail.net.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27122253

Citation

Chang, Dong-Yeop, et al. "Interleukin-4 Enzyme-linked Immunospot Assay May Be Useful for Diagnosing Sensitization to House Dust Mite." International Forum of Allergy & Rhinology, vol. 6, no. 10, 2016, pp. 1007-1012.
Chang DY, Lee J, Choi SW, et al. Interleukin-4 enzyme-linked immunospot assay may be useful for diagnosing sensitization to house dust mite. Int Forum Allergy Rhinol. 2016;6(10):1007-1012.
Chang, D. Y., Lee, J., Choi, S. W., Lee, H. J., Kang, H., Yeo, S. C., Joo, Y. H., Cho, H. J., Jeon, S. Y., Kim, R. B., & Kim, S. W. (2016). Interleukin-4 enzyme-linked immunospot assay may be useful for diagnosing sensitization to house dust mite. International Forum of Allergy & Rhinology, 6(10), 1007-1012. https://doi.org/10.1002/alr.21786
Chang DY, et al. Interleukin-4 Enzyme-linked Immunospot Assay May Be Useful for Diagnosing Sensitization to House Dust Mite. Int Forum Allergy Rhinol. 2016;6(10):1007-1012. PubMed PMID: 27122253.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Interleukin-4 enzyme-linked immunospot assay may be useful for diagnosing sensitization to house dust mite. AU - Chang,Dong-Yeop, AU - Lee,Jino, AU - Choi,Sung-Won, AU - Lee,Hyeong Joo, AU - Kang,Hyunmo, AU - Yeo,Seong Chul, AU - Joo,Yeon-Hee, AU - Cho,Hyun-Jin, AU - Jeon,Sea-Yuong, AU - Kim,Rock Bum, AU - Kim,Sang-Wook, Y1 - 2016/04/28/ PY - 2015/11/10/received PY - 2016/02/18/revised PY - 2016/03/08/accepted PY - 2016/4/29/pubmed PY - 2017/11/10/medline PY - 2016/4/29/entrez KW - allergic rhinitis KW - allergy KW - enzyme-linked immunospot assay KW - house dust mites KW - interleukin-4 SP - 1007 EP - 1012 JF - International forum of allergy & rhinology JO - Int Forum Allergy Rhinol VL - 6 IS - 10 N2 - BACKGROUND: The skin prick test (SPT) is considered a standard test for identification of allergens, but it has some limitations in clinical practice. The multiple allergen simultaneous test (MAST), which measures allergen-specific immunoglobulin E in patients' serum, is a widely used alternative test, but is limited by its relatively low sensitivity and specificity. As a novel diagnostic test to identify allergens, we investigated the sensitivity and specificity of an interleukin-4 (IL-4) enzyme-linked immunospot (ELISpot) assay for Dermatophagoides farinae (Der f) and Dermatophagoides pteronyssinus (Der p). METHODS: Based on the symptoms and SPT results, 43 house dust mite (HDM) allergic rhinitis (AR) patients and 41 control subjects were included. Peripheral blood was drawn from each subject for IL-4 ELISpot assay and MAST. The receiver operating characteristic (ROC) curve analysis was conducted to determine the cutoff values. Sensitivity, specificity, and positive and neg predictive values were compared between the 2 tests. RESULTS: The sensitivity, specificity, and areas under the ROC curve (AUCs) of the IL-4 ELISpot assay were 88.4%, 97.6%, and 0.939 for Der f, and 95.3%, 97.5%, and 0.971 for Der p, respectively. However, the sensitivity, specificity, and AUC of MAST were 76.7%, 73.2%, and 0.777 for Der f, and 69.8%, 75.6%, and 0.788 for Der p, respectively. CONCLUSION: The IL-4 ELISpot assay showed higher sensitivity, specificity, and AUC than MAST, which indicates its clinical feasibility for diagnosing allergy for HDM. A further study is needed to determine the accuracy of the IL-4 ELISpot assay for other common allergens. SN - 2042-6984 UR - https://www.unboundmedicine.com/medline/citation/27122253/Interleukin_4_enzyme_linked_immunospot_assay_may_be_useful_for_diagnosing_sensitization_to_house_dust_mite_ L2 - https://doi.org/10.1002/alr.21786 DB - PRIME DP - Unbound Medicine ER -