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Optimization of microwave-assisted extraction and pressurized liquid extraction of phenolic compounds from Moringa oleifera leaves by multiresponse surface methodology.
Electrophoresis 2016; 37(13):1938-46E

Abstract

This work aims at studying the optimization of microwave-assisted extraction (MAE) and pressurized liquid extraction (PLE) by multi-response surface methodology (RSM) to test their efficiency towards the extraction of phenolic compounds from Moringa oleifera (M. oleifera) leaves. The extraction yield, total phenolic content (TPC), total flavonoid content (TF), DPPH scavenging method and trolox equivalent antioxidant capacity (TEAC) assay were considered as response variables while effects of extraction time, percentage of ethanol, and temperature were studied. Extraction time of 20 min, 42% ethanol and 158°C were the MAE optimum conditions for achieving extraction yield of 26 ± 2%, EC50 15 ± 2 μg/mL, 16 ± 1 Eq Trolox/100 g dry leaf, 5.2 ± 0.5 mg Eq quercetin/g dry leaf, and 86 ± 4 mg GAE/g dry leaf. Regarding PLE, the optimum conditions that allowed extraction yield of 56 ± 2%, EC50 21 ± 3 μg/mL, 12 ± 2 mmol Eq Trolox/100 g dry leaf, 6.5 ± 0.2 mg Eq quercetin/g dry leaf, and 59 ± 6 mg GAE/g dry leaf were 128°C, 35% of ethanol, and 20 min. PLE enabled the extraction of phenolic compounds with a higher number of hydroxyl-type substituents such as kaempferol diglycoside and its acetyl derivatives and those that are sensitive to high temperatures (glucosinolates or amino acids) while MAE allowed better recoveries of kaempferol, quercetin, and their glucosides derivatives.

Authors+Show Affiliations

Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Granada, Spain. Research and Development Functional Food Centre (CIDAF), Health Science Technological Park, Granada, Spain.Foodomics Laboratory, Bioactivity and Food Analysis Department, Institute of Food Science Research (CIAL-CSIC), Madrid, Spain.Foodomics Laboratory, Bioactivity and Food Analysis Department, Institute of Food Science Research (CIAL-CSIC), Madrid, Spain.Research and Development Functional Food Centre (CIDAF), Health Science Technological Park, Granada, Spain.Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Granada, Spain. Research and Development Functional Food Centre (CIDAF), Health Science Technological Park, Granada, Spain.Foodomics Laboratory, Bioactivity and Food Analysis Department, Institute of Food Science Research (CIAL-CSIC), Madrid, Spain.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27122439

Citation

Rodríguez-Pérez, Celia, et al. "Optimization of Microwave-assisted Extraction and Pressurized Liquid Extraction of Phenolic Compounds From Moringa Oleifera Leaves By Multiresponse Surface Methodology." Electrophoresis, vol. 37, no. 13, 2016, pp. 1938-46.
Rodríguez-Pérez C, Gilbert-López B, Mendiola JA, et al. Optimization of microwave-assisted extraction and pressurized liquid extraction of phenolic compounds from Moringa oleifera leaves by multiresponse surface methodology. Electrophoresis. 2016;37(13):1938-46.
Rodríguez-Pérez, C., Gilbert-López, B., Mendiola, J. A., Quirantes-Piné, R., Segura-Carretero, A., & Ibáñez, E. (2016). Optimization of microwave-assisted extraction and pressurized liquid extraction of phenolic compounds from Moringa oleifera leaves by multiresponse surface methodology. Electrophoresis, 37(13), pp. 1938-46. doi:10.1002/elps.201600071.
Rodríguez-Pérez C, et al. Optimization of Microwave-assisted Extraction and Pressurized Liquid Extraction of Phenolic Compounds From Moringa Oleifera Leaves By Multiresponse Surface Methodology. Electrophoresis. 2016;37(13):1938-46. PubMed PMID: 27122439.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Optimization of microwave-assisted extraction and pressurized liquid extraction of phenolic compounds from Moringa oleifera leaves by multiresponse surface methodology. AU - Rodríguez-Pérez,Celia, AU - Gilbert-López,Bienvenida, AU - Mendiola,Jose Antonio, AU - Quirantes-Piné,Rosa, AU - Segura-Carretero,Antonio, AU - Ibáñez,Elena, Y1 - 2016/06/06/ PY - 2016/02/17/received PY - 2016/04/04/revised PY - 2016/04/04/accepted PY - 2016/4/29/entrez PY - 2016/4/29/pubmed PY - 2017/6/24/medline KW - HPLC-ESI-QTOF-MS KW - Microwave-assisted extraction KW - Moringa oleifera KW - Pressurized liquid extraction KW - Response-surface methodology SP - 1938 EP - 46 JF - Electrophoresis JO - Electrophoresis VL - 37 IS - 13 N2 - This work aims at studying the optimization of microwave-assisted extraction (MAE) and pressurized liquid extraction (PLE) by multi-response surface methodology (RSM) to test their efficiency towards the extraction of phenolic compounds from Moringa oleifera (M. oleifera) leaves. The extraction yield, total phenolic content (TPC), total flavonoid content (TF), DPPH scavenging method and trolox equivalent antioxidant capacity (TEAC) assay were considered as response variables while effects of extraction time, percentage of ethanol, and temperature were studied. Extraction time of 20 min, 42% ethanol and 158°C were the MAE optimum conditions for achieving extraction yield of 26 ± 2%, EC50 15 ± 2 μg/mL, 16 ± 1 Eq Trolox/100 g dry leaf, 5.2 ± 0.5 mg Eq quercetin/g dry leaf, and 86 ± 4 mg GAE/g dry leaf. Regarding PLE, the optimum conditions that allowed extraction yield of 56 ± 2%, EC50 21 ± 3 μg/mL, 12 ± 2 mmol Eq Trolox/100 g dry leaf, 6.5 ± 0.2 mg Eq quercetin/g dry leaf, and 59 ± 6 mg GAE/g dry leaf were 128°C, 35% of ethanol, and 20 min. PLE enabled the extraction of phenolic compounds with a higher number of hydroxyl-type substituents such as kaempferol diglycoside and its acetyl derivatives and those that are sensitive to high temperatures (glucosinolates or amino acids) while MAE allowed better recoveries of kaempferol, quercetin, and their glucosides derivatives. SN - 1522-2683 UR - https://www.unboundmedicine.com/medline/citation/27122439/Optimization_of_microwave_assisted_extraction_and_pressurized_liquid_extraction_of_phenolic_compounds_from_Moringa_oleifera_leaves_by_multiresponse_surface_methodology_ L2 - https://doi.org/10.1002/elps.201600071 DB - PRIME DP - Unbound Medicine ER -