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Genetic analysis of a novel broad-spectrum powdery mildew resistance gene from the wheat-Agropyron cristatum introgression line Pubing 74.
Planta. 2016 Sep; 244(3):713-23.P

Abstract

MAIN CONCLUSION

A novel broad-spectrum powdery mildew resistance gene PmPB74 was identified in wheat- Agropyron cristatum introgression line Pubing 74. Development of wheat cultivars with broad-spectrum, durable resistance to powdery mildew has been restricted by lack of superior genetic resources. In this study, a wheat-A. cristatum introgression line Pubing 74, originally selected from a wide cross between the common wheat cultivar Fukuhokomugi (Fukuho) and Agropyron cristatum (L.) Gaertn (2n = 4x = 28; genome PPPP), displayed resistance to powdery mildew at both the seedling and adult stages. The putative alien chromosomal fragment in Pubing 74 was below the detection limit of genomic in situ hybridization (GISH), but evidence for other non-GISH-detectable introgressions was provided by the presence of three STS markers specific to A. cristatum. Genetic analysis indicated that Pubing 74 carried a single dominant gene for powdery mildew resistance, temporarily designated PmPB74. Molecular mapping showed that PmPB74 was located on wheat chromosome arm 5DS, and flanked by markers Xcfd81 and HRM02 at genetic distances of 2.5 and 1.7 cM, respectively. Compared with other lines with powdery mildew resistance gene(s) on wheat chromosome arm 5DS, Pubing 74 was resistant to all 28 Blumeria graminis f. sp tritici (Bgt) isolates from different wheat-producing regions of northern China. Allelism tests indicated that PmPB74 was not allelic to PmPB3558 or Pm2. Our work showed that PmPB74 is a novel gene with broad resistance to powdery mildew, and hence will be helpful in broadening the genetic basis of powdery mildew resistance in wheat.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Lu Y
National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Yao M
National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Zhang J
National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Song L
National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Liu W
National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Yang X
National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Li X
National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Li L
National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China. lilihui@caas.cn.

MeSH

AgropyronAscomycotaChromosome MappingDisease ResistanceHost-Pathogen InteractionsTriticum

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27125388

Citation

Lu, Yuqing, et al. "Genetic Analysis of a Novel Broad-spectrum Powdery Mildew Resistance Gene From the wheat-Agropyron Cristatum Introgression Line Pubing 74." Planta, vol. 244, no. 3, 2016, pp. 713-23.
Lu Y, Yao M, Zhang J, et al. Genetic analysis of a novel broad-spectrum powdery mildew resistance gene from the wheat-Agropyron cristatum introgression line Pubing 74. Planta. 2016;244(3):713-23.
Lu, Y., Yao, M., Zhang, J., Song, L., Liu, W., Yang, X., Li, X., & Li, L. (2016). Genetic analysis of a novel broad-spectrum powdery mildew resistance gene from the wheat-Agropyron cristatum introgression line Pubing 74. Planta, 244(3), 713-23. https://doi.org/10.1007/s00425-016-2538-y
Lu Y, et al. Genetic Analysis of a Novel Broad-spectrum Powdery Mildew Resistance Gene From the wheat-Agropyron Cristatum Introgression Line Pubing 74. Planta. 2016;244(3):713-23. PubMed PMID: 27125388.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genetic analysis of a novel broad-spectrum powdery mildew resistance gene from the wheat-Agropyron cristatum introgression line Pubing 74. AU - Lu,Yuqing, AU - Yao,Miaomiao, AU - Zhang,Jinpeng, AU - Song,Liqiang, AU - Liu,Weihua, AU - Yang,Xinming, AU - Li,Xiuquan, AU - Li,Lihui, Y1 - 2016/04/28/ PY - 2016/01/20/received PY - 2016/04/20/accepted PY - 2016/4/30/entrez PY - 2016/4/30/pubmed PY - 2017/3/31/medline KW - Blumeria graminis f. sp. tritici KW - Disease resistance KW - Triticum aestivum KW - Wide cross SP - 713 EP - 23 JF - Planta JO - Planta VL - 244 IS - 3 N2 - MAIN CONCLUSION: A novel broad-spectrum powdery mildew resistance gene PmPB74 was identified in wheat- Agropyron cristatum introgression line Pubing 74. Development of wheat cultivars with broad-spectrum, durable resistance to powdery mildew has been restricted by lack of superior genetic resources. In this study, a wheat-A. cristatum introgression line Pubing 74, originally selected from a wide cross between the common wheat cultivar Fukuhokomugi (Fukuho) and Agropyron cristatum (L.) Gaertn (2n = 4x = 28; genome PPPP), displayed resistance to powdery mildew at both the seedling and adult stages. The putative alien chromosomal fragment in Pubing 74 was below the detection limit of genomic in situ hybridization (GISH), but evidence for other non-GISH-detectable introgressions was provided by the presence of three STS markers specific to A. cristatum. Genetic analysis indicated that Pubing 74 carried a single dominant gene for powdery mildew resistance, temporarily designated PmPB74. Molecular mapping showed that PmPB74 was located on wheat chromosome arm 5DS, and flanked by markers Xcfd81 and HRM02 at genetic distances of 2.5 and 1.7 cM, respectively. Compared with other lines with powdery mildew resistance gene(s) on wheat chromosome arm 5DS, Pubing 74 was resistant to all 28 Blumeria graminis f. sp tritici (Bgt) isolates from different wheat-producing regions of northern China. Allelism tests indicated that PmPB74 was not allelic to PmPB3558 or Pm2. Our work showed that PmPB74 is a novel gene with broad resistance to powdery mildew, and hence will be helpful in broadening the genetic basis of powdery mildew resistance in wheat. SN - 1432-2048 UR - https://www.unboundmedicine.com/medline/citation/27125388/Genetic_analysis_of_a_novel_broad_spectrum_powdery_mildew_resistance_gene_from_the_wheat_Agropyron_cristatum_introgression_line_Pubing_74_ DB - PRIME DP - Unbound Medicine ER -