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The effect of temperature on proliferation and differentiation of chicken skeletal muscle satellite cells isolated from different muscle types.
Physiol Rep. 2016 Apr; 4(8)PR

Abstract

Skeletal muscle satellite cells are a muscle stem cell population that mediate posthatch muscle growth and repair. Satellite cells respond differentially to environmental stimuli based upon their fiber-type of origin. The objective of this study was to determine how temperatures below and above the in vitro control of 38°C affected the proliferation and differentiation of satellite cells isolated from the chicken anaerobic pectoralis major (p. major) or mixed fiber biceps femoris (b.femoris) muscles. The satellite cells isolated from the p. major muscle were more sensitive to both cold and hot temperatures compared to the b.femoris satellite cells during both proliferation and differentiation. The expressions of myogenic regulatory transcription factors were also different between satellite cells from different fiber types. MyoD expression, which partially regulates proliferation, was generally expressed at higher levels in p. major satellite cells compared to the b.femoris satellite cells from 33 to 43°C during proliferation and differentiation. Similarly, myogenin expression, which is required for differentiation, was also expressed at higher levels in p. major satellite cells in response to both cold and hot temperatures during proliferation and differentiation than b. femoris satellite cells. These data demonstrate that satellite cells from the anaerobic p. major muscle are more sensitive than satellite cells from the aerobic b. femoris muscle to both hot and cold thermal stress during myogenic proliferation and differentiation.

Authors+Show Affiliations

Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, Ohio.Department of Animal Sciences, The Hebrew University of Jerusalem, Rehovot, Israel.Institute of Animal Sciences, Agricultural Research Organization The Volcani Center, Bet Dagan, Israel.Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, Ohio Velleman.1@osu.edu.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27125667

Citation

Harding, Rachel L., et al. "The Effect of Temperature On Proliferation and Differentiation of Chicken Skeletal Muscle Satellite Cells Isolated From Different Muscle Types." Physiological Reports, vol. 4, no. 8, 2016.
Harding RL, Halevy O, Yahav S, et al. The effect of temperature on proliferation and differentiation of chicken skeletal muscle satellite cells isolated from different muscle types. Physiol Rep. 2016;4(8).
Harding, R. L., Halevy, O., Yahav, S., & Velleman, S. G. (2016). The effect of temperature on proliferation and differentiation of chicken skeletal muscle satellite cells isolated from different muscle types. Physiological Reports, 4(8). https://doi.org/10.14814/phy2.12770
Harding RL, et al. The Effect of Temperature On Proliferation and Differentiation of Chicken Skeletal Muscle Satellite Cells Isolated From Different Muscle Types. Physiol Rep. 2016;4(8) PubMed PMID: 27125667.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The effect of temperature on proliferation and differentiation of chicken skeletal muscle satellite cells isolated from different muscle types. AU - Harding,Rachel L, AU - Halevy,Orna, AU - Yahav,Shlomo, AU - Velleman,Sandra G, Y1 - 2016/04/27/ PY - 2016/02/09/received PY - 2016/03/31/accepted PY - 2016/4/30/entrez PY - 2016/4/30/pubmed PY - 2016/4/30/medline KW - Chicken KW - fiber type KW - muscle KW - satellite cells KW - temperature JF - Physiological reports JO - Physiol Rep VL - 4 IS - 8 N2 - Skeletal muscle satellite cells are a muscle stem cell population that mediate posthatch muscle growth and repair. Satellite cells respond differentially to environmental stimuli based upon their fiber-type of origin. The objective of this study was to determine how temperatures below and above the in vitro control of 38°C affected the proliferation and differentiation of satellite cells isolated from the chicken anaerobic pectoralis major (p. major) or mixed fiber biceps femoris (b.femoris) muscles. The satellite cells isolated from the p. major muscle were more sensitive to both cold and hot temperatures compared to the b.femoris satellite cells during both proliferation and differentiation. The expressions of myogenic regulatory transcription factors were also different between satellite cells from different fiber types. MyoD expression, which partially regulates proliferation, was generally expressed at higher levels in p. major satellite cells compared to the b.femoris satellite cells from 33 to 43°C during proliferation and differentiation. Similarly, myogenin expression, which is required for differentiation, was also expressed at higher levels in p. major satellite cells in response to both cold and hot temperatures during proliferation and differentiation than b. femoris satellite cells. These data demonstrate that satellite cells from the anaerobic p. major muscle are more sensitive than satellite cells from the aerobic b. femoris muscle to both hot and cold thermal stress during myogenic proliferation and differentiation. SN - 2051-817X UR - https://www.unboundmedicine.com/medline/citation/27125667/The_effect_of_temperature_on_proliferation_and_differentiation_of_chicken_skeletal_muscle_satellite_cells_isolated_from_different_muscle_types_ L2 - https://doi.org/10.14814/phy2.12770 DB - PRIME DP - Unbound Medicine ER -