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Sesamin inhibits lipopolysaccharide-induced inflammation and extracellular matrix catabolism in rat intervertebral disc.
Connect Tissue Res. 2016 09; 57(5):347-59.CT

Abstract

Intervertebral disc (IVD) degeneration contributes to most spinal degenerative diseases, while treatment inhibiting IVD degeneration is still in the experimental stage. Sesamin, a bioactive component extracted from sesame, has been reported to exert chondroprotective and anti-inflammatory effects. Here, we analyzed the anti-inflammatory and anti-catabolic effects of sesamin on rat IVD in vitro and ex vivo. Results show that sesamin significantly inhibits the lipopolysaccharide (LPS)-induced expression of catabolic enzymes (MMP-1, MMP-3, MMP-13, ADAMTS-4, ADAMTS-5) and inflammation factors (IL-1β, TNF-α, iNOS, NO, COX-2, PGE2) in a dose-dependent manner in vitro. It is also proven that migration of macrophages induced by LPS can be inhibited by treatment with sesamin. Organ culture experiments demonstrate that sesamin protects the IVD from LPS-induced depletion of the extracellular matrix ex vivo. Moreover, sesamin suppresses LPS-induced activation of the mitogen-activated protein kinase (MAPK) pathway through inhibiting phosphorylation of JNK, the common downstream signaling pathway of LPS and IL-1β, which may be the potential mechanism of the effects of sesamin. In light of our results, sesamin protects the IVD from inflammation and extracellular matrix catabolism, presenting positive prospects in the treatment of IVD degenerative diseases.

Authors+Show Affiliations

a Shanghai Key Laboratory of Orthopaedic Implant, Department of Orthopaedic Surgery , Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine , Shanghai , China.a Shanghai Key Laboratory of Orthopaedic Implant, Department of Orthopaedic Surgery , Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine , Shanghai , China.a Shanghai Key Laboratory of Orthopaedic Implant, Department of Orthopaedic Surgery , Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine , Shanghai , China.a Shanghai Key Laboratory of Orthopaedic Implant, Department of Orthopaedic Surgery , Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine , Shanghai , China.a Shanghai Key Laboratory of Orthopaedic Implant, Department of Orthopaedic Surgery , Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine , Shanghai , China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27128308

Citation

Li, Kang, et al. "Sesamin Inhibits Lipopolysaccharide-induced Inflammation and Extracellular Matrix Catabolism in Rat Intervertebral Disc." Connective Tissue Research, vol. 57, no. 5, 2016, pp. 347-59.
Li K, Li Y, Xu B, et al. Sesamin inhibits lipopolysaccharide-induced inflammation and extracellular matrix catabolism in rat intervertebral disc. Connect Tissue Res. 2016;57(5):347-59.
Li, K., Li, Y., Xu, B., Mao, L., & Zhao, J. (2016). Sesamin inhibits lipopolysaccharide-induced inflammation and extracellular matrix catabolism in rat intervertebral disc. Connective Tissue Research, 57(5), 347-59. https://doi.org/10.1080/03008207.2016.1182998
Li K, et al. Sesamin Inhibits Lipopolysaccharide-induced Inflammation and Extracellular Matrix Catabolism in Rat Intervertebral Disc. Connect Tissue Res. 2016;57(5):347-59. PubMed PMID: 27128308.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sesamin inhibits lipopolysaccharide-induced inflammation and extracellular matrix catabolism in rat intervertebral disc. AU - Li,Kang, AU - Li,Yan, AU - Xu,Bo, AU - Mao,Lu, AU - Zhao,Jie, Y1 - 2016/04/29/ PY - 2016/4/30/entrez PY - 2016/4/30/pubmed PY - 2017/9/5/medline KW - extracellular matrix KW - inflammation KW - intervertebral disc KW - lipopolysaccharide KW - organ culture KW - sesamin SP - 347 EP - 59 JF - Connective tissue research JO - Connect. Tissue Res. VL - 57 IS - 5 N2 - Intervertebral disc (IVD) degeneration contributes to most spinal degenerative diseases, while treatment inhibiting IVD degeneration is still in the experimental stage. Sesamin, a bioactive component extracted from sesame, has been reported to exert chondroprotective and anti-inflammatory effects. Here, we analyzed the anti-inflammatory and anti-catabolic effects of sesamin on rat IVD in vitro and ex vivo. Results show that sesamin significantly inhibits the lipopolysaccharide (LPS)-induced expression of catabolic enzymes (MMP-1, MMP-3, MMP-13, ADAMTS-4, ADAMTS-5) and inflammation factors (IL-1β, TNF-α, iNOS, NO, COX-2, PGE2) in a dose-dependent manner in vitro. It is also proven that migration of macrophages induced by LPS can be inhibited by treatment with sesamin. Organ culture experiments demonstrate that sesamin protects the IVD from LPS-induced depletion of the extracellular matrix ex vivo. Moreover, sesamin suppresses LPS-induced activation of the mitogen-activated protein kinase (MAPK) pathway through inhibiting phosphorylation of JNK, the common downstream signaling pathway of LPS and IL-1β, which may be the potential mechanism of the effects of sesamin. In light of our results, sesamin protects the IVD from inflammation and extracellular matrix catabolism, presenting positive prospects in the treatment of IVD degenerative diseases. SN - 1607-8438 UR - https://www.unboundmedicine.com/medline/citation/27128308/Sesamin_inhibits_lipopolysaccharide_induced_inflammation_and_extracellular_matrix_catabolism_in_rat_intervertebral_disc_ L2 - http://www.tandfonline.com/doi/full/10.1080/03008207.2016.1182998 DB - PRIME DP - Unbound Medicine ER -