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Nrf2/antioxidant defense pathway is involved in the neuroprotective effects of Sirt1 against focal cerebral ischemia in rats after hyperbaric oxygen preconditioning.
Behav Brain Res. 2016 08 01; 309:1-8.BB

Abstract

Sirtuin 1 (Sirt1) is a class III histone deacetylase involved in neuroprotection induced by hyperbaric oxygen preconditioning (HBO-PC) in animal models of ischemia. However, the underlying mechanisms remain to be illustrated. In the present study, rats exposed to middle cerebral artery occlusion (MCAO) were used to establish an ischemic stroke model. The infarct volume ratio, neurobehavioral score, and expressions of Sirt1, nuclear factor erythroid 2-related factor 2 (Nrf2), heme oxygenase 1 (HO-1), and superoxide dismutase 1 (SOD1) were evaluated at 7 days after reperfusion, and the level of malondialdehyde (MDA) was used to assess oxidative stress. HBO-PC increased the expression of Sirt1 and reduced infarct volume ratio and neurobehavioral deficit in MCAO rats. Meanwhile, HBO-PC also increased expression of Nrf2, HO-1, and SOD1 and decreased MDA content. Furthermore, either Sirt1 or Nrf2 knockdown by short interfering RNA (siRNA) inhibited the expression of Nrf2, HO-1, and SOD1 and eliminated the neuroprotective effects of HBO-PC. Taken together, the results suggest that the Nrf2/antioxidant defense pathway is involved in the long lasting neuroprotective effects of Sirt1 induced by HBO-PC against transient focal cerebral ischemia.

Authors+Show Affiliations

Department of Psychiatry, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.Department of Anesthesiology, Gansu Provincial Hospital, Lanzhou, China.Department of Anesthesiology, Gansu Provincial Hospital, Lanzhou, China.Department of Anesthesiology, Gansu Provincial Hospital, Lanzhou, China.Department of Anesthesiology, Gansu Provincial Hospital, Lanzhou, China.Department of Anesthesiology, Gansu Provincial Hospital, Lanzhou, China.College of Life Science, Shaanxi Normal University, China.Department of Psychiatry, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China. Electronic address: pengzhengwu1446@163.com.Department of Anesthesiology, Gansu Provincial Hospital, Lanzhou, China. Electronic address: gsywj2008@hotmail.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27131779

Citation

Xue, Fen, et al. "Nrf2/antioxidant Defense Pathway Is Involved in the Neuroprotective Effects of Sirt1 Against Focal Cerebral Ischemia in Rats After Hyperbaric Oxygen Preconditioning." Behavioural Brain Research, vol. 309, 2016, pp. 1-8.
Xue F, Huang JW, Ding PY, et al. Nrf2/antioxidant defense pathway is involved in the neuroprotective effects of Sirt1 against focal cerebral ischemia in rats after hyperbaric oxygen preconditioning. Behav Brain Res. 2016;309:1-8.
Xue, F., Huang, J. W., Ding, P. Y., Zang, H. G., Kou, Z. J., Li, T., Fan, J., Peng, Z. W., & Yan, W. J. (2016). Nrf2/antioxidant defense pathway is involved in the neuroprotective effects of Sirt1 against focal cerebral ischemia in rats after hyperbaric oxygen preconditioning. Behavioural Brain Research, 309, 1-8. https://doi.org/10.1016/j.bbr.2016.04.045
Xue F, et al. Nrf2/antioxidant Defense Pathway Is Involved in the Neuroprotective Effects of Sirt1 Against Focal Cerebral Ischemia in Rats After Hyperbaric Oxygen Preconditioning. Behav Brain Res. 2016 08 1;309:1-8. PubMed PMID: 27131779.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nrf2/antioxidant defense pathway is involved in the neuroprotective effects of Sirt1 against focal cerebral ischemia in rats after hyperbaric oxygen preconditioning. AU - Xue,Fen, AU - Huang,Jin-Wen, AU - Ding,Pei-Yan, AU - Zang,Hong-Gang, AU - Kou,Zhi-Jian, AU - Li,Ting, AU - Fan,Juan, AU - Peng,Zheng-Wu, AU - Yan,Wen-Jun, Y1 - 2016/04/27/ PY - 2016/02/04/received PY - 2016/04/24/revised PY - 2016/04/26/accepted PY - 2016/5/2/entrez PY - 2016/5/2/pubmed PY - 2017/11/29/medline KW - HBO-PC KW - MCAO KW - Nrf2 KW - Oxidative stress KW - Sirt1 SP - 1 EP - 8 JF - Behavioural brain research JO - Behav. Brain Res. VL - 309 N2 - Sirtuin 1 (Sirt1) is a class III histone deacetylase involved in neuroprotection induced by hyperbaric oxygen preconditioning (HBO-PC) in animal models of ischemia. However, the underlying mechanisms remain to be illustrated. In the present study, rats exposed to middle cerebral artery occlusion (MCAO) were used to establish an ischemic stroke model. The infarct volume ratio, neurobehavioral score, and expressions of Sirt1, nuclear factor erythroid 2-related factor 2 (Nrf2), heme oxygenase 1 (HO-1), and superoxide dismutase 1 (SOD1) were evaluated at 7 days after reperfusion, and the level of malondialdehyde (MDA) was used to assess oxidative stress. HBO-PC increased the expression of Sirt1 and reduced infarct volume ratio and neurobehavioral deficit in MCAO rats. Meanwhile, HBO-PC also increased expression of Nrf2, HO-1, and SOD1 and decreased MDA content. Furthermore, either Sirt1 or Nrf2 knockdown by short interfering RNA (siRNA) inhibited the expression of Nrf2, HO-1, and SOD1 and eliminated the neuroprotective effects of HBO-PC. Taken together, the results suggest that the Nrf2/antioxidant defense pathway is involved in the long lasting neuroprotective effects of Sirt1 induced by HBO-PC against transient focal cerebral ischemia. SN - 1872-7549 UR - https://www.unboundmedicine.com/medline/citation/27131779/Nrf2/antioxidant_defense_pathway_is_involved_in_the_neuroprotective_effects_of_Sirt1_against_focal_cerebral_ischemia_in_rats_after_hyperbaric_oxygen_preconditioning_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0166-4328(16)30260-1 DB - PRIME DP - Unbound Medicine ER -