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The potential mechanism of Bawei Xileisan in the treatment of dextran sulfate sodium-induced ulcerative colitis in mice.
J Ethnopharmacol 2016; 188:31-8JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Bawei Xileisan (BXS), a traditional Chinese compound medicine, has been historically used in the treatment of ulcers and inflammation. BXS is also used as a topical agent for the treatment of ulcerative colitis in China. The underlying mechanism, however, remains elusive.

MATERIALS AND METHODS

Thirty-six female C57BL/6 mice with average weight of 20±2g were used for an in vivo study. The present work was conducted in accordance with the protocols approved by the Ethics Committee of Animal Experiments of Lanzhou University. The mice were induced to develop acute colitis by treating these with 3% dextran sulfate sodium (DSS) solution for 5 days. Subsequently, BXS (200,400mg/kg) was rectally administered daily for one week. All mice were killed at day 12 and their body weight, colon length, and histological changes were all recorded. Serum T helper 17 (Th17) cytokine levels were determined by enzyme-linked immunosorbent assay (ELISA). Th17 and regulatory T cell (Treg) in splenocyte mononuclear cells were isolated and identified via flow cytometry. Stool DNA was extracted and the absolute number of Bacteroides and Lactobacillus were measured by using real-time Q-PCR.

RESULTS

Shortened colon and damaged tissue structure were profoundly ameliorated by BXS enema. The expression level of Th17-related cytokines IL-17A/F and IL-22 was significantly and dose-dependently reduced, resulting in the restoration of Th17/Treg balance. Moreover, BXS also improved the feces Lactobacillus levels and manifested beneficial effects on Bacteroides.

CONCLUSIONS

The findings of the present study suggest that BXS is curative in a mouse model of ulcerative colitis, and the underlying mechanism might involve disruption of the Th17 pathway and the induction of a Th17/Treg imbalance, as well as an the development of an opsonic effect on specific gut microbiota.

Authors+Show Affiliations

School of Life Sciences, Lanzhou University, Lanzhou 730000, China; Gansu Key Laboratory of Functional Genomics and Molecular Diagnosis, Lanzhou 730000, China; Key Laboratory of Cell Activities and Stress Adaptations, Ministry of Education, Lanzhou University, Lanzhou 730000, China.Gansu Institute of Drug Control, Lanzhou 730000, China.Gansu Institute of Drug Control, Lanzhou 730000, China.School of Life Sciences, Lanzhou University, Lanzhou 730000, China; Gansu Key Laboratory of Functional Genomics and Molecular Diagnosis, Lanzhou 730000, China; Key Laboratory of Cell Activities and Stress Adaptations, Ministry of Education, Lanzhou University, Lanzhou 730000, China.School of Life Sciences, Lanzhou University, Lanzhou 730000, China; Gansu Key Laboratory of Functional Genomics and Molecular Diagnosis, Lanzhou 730000, China; Key Laboratory of Cell Activities and Stress Adaptations, Ministry of Education, Lanzhou University, Lanzhou 730000, China.School of Life Sciences, Lanzhou University, Lanzhou 730000, China; Gansu Key Laboratory of Functional Genomics and Molecular Diagnosis, Lanzhou 730000, China; Key Laboratory of Cell Activities and Stress Adaptations, Ministry of Education, Lanzhou University, Lanzhou 730000, China.School of Life Sciences, Lanzhou University, Lanzhou 730000, China; Gansu Key Laboratory of Functional Genomics and Molecular Diagnosis, Lanzhou 730000, China; Key Laboratory of Cell Activities and Stress Adaptations, Ministry of Education, Lanzhou University, Lanzhou 730000, China.School of Life Sciences, Lanzhou University, Lanzhou 730000, China; Gansu Key Laboratory of Functional Genomics and Molecular Diagnosis, Lanzhou 730000, China; Key Laboratory of Cell Activities and Stress Adaptations, Ministry of Education, Lanzhou University, Lanzhou 730000, China. Electronic address: chjzh@lzu.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27132718

Citation

Wen, Juan, et al. "The Potential Mechanism of Bawei Xileisan in the Treatment of Dextran Sulfate Sodium-induced Ulcerative Colitis in Mice." Journal of Ethnopharmacology, vol. 188, 2016, pp. 31-8.
Wen J, Teng B, Yang P, et al. The potential mechanism of Bawei Xileisan in the treatment of dextran sulfate sodium-induced ulcerative colitis in mice. J Ethnopharmacol. 2016;188:31-8.
Wen, J., Teng, B., Yang, P., Chen, X., Li, C., Jing, Y., ... Zhang, C. (2016). The potential mechanism of Bawei Xileisan in the treatment of dextran sulfate sodium-induced ulcerative colitis in mice. Journal of Ethnopharmacology, 188, pp. 31-8. doi:10.1016/j.jep.2016.04.054.
Wen J, et al. The Potential Mechanism of Bawei Xileisan in the Treatment of Dextran Sulfate Sodium-induced Ulcerative Colitis in Mice. J Ethnopharmacol. 2016 Jul 21;188:31-8. PubMed PMID: 27132718.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The potential mechanism of Bawei Xileisan in the treatment of dextran sulfate sodium-induced ulcerative colitis in mice. AU - Wen,Juan, AU - Teng,Baoxia, AU - Yang,Pingrong, AU - Chen,Xinjun, AU - Li,Chenhui, AU - Jing,Yaping, AU - Wei,Junshu, AU - Zhang,Chunjiang, Y1 - 2016/04/29/ PY - 2015/10/30/received PY - 2016/04/26/revised PY - 2016/04/28/accepted PY - 2016/5/3/entrez PY - 2016/5/3/pubmed PY - 2017/4/22/medline KW - Bawei Xileisan KW - Dextran Sulfate KW - Gut Microbiota KW - Sodium KW - Th17/Treg KW - Traditional Chinese medicine KW - ulcerative Colitis SP - 31 EP - 8 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 188 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Bawei Xileisan (BXS), a traditional Chinese compound medicine, has been historically used in the treatment of ulcers and inflammation. BXS is also used as a topical agent for the treatment of ulcerative colitis in China. The underlying mechanism, however, remains elusive. MATERIALS AND METHODS: Thirty-six female C57BL/6 mice with average weight of 20±2g were used for an in vivo study. The present work was conducted in accordance with the protocols approved by the Ethics Committee of Animal Experiments of Lanzhou University. The mice were induced to develop acute colitis by treating these with 3% dextran sulfate sodium (DSS) solution for 5 days. Subsequently, BXS (200,400mg/kg) was rectally administered daily for one week. All mice were killed at day 12 and their body weight, colon length, and histological changes were all recorded. Serum T helper 17 (Th17) cytokine levels were determined by enzyme-linked immunosorbent assay (ELISA). Th17 and regulatory T cell (Treg) in splenocyte mononuclear cells were isolated and identified via flow cytometry. Stool DNA was extracted and the absolute number of Bacteroides and Lactobacillus were measured by using real-time Q-PCR. RESULTS: Shortened colon and damaged tissue structure were profoundly ameliorated by BXS enema. The expression level of Th17-related cytokines IL-17A/F and IL-22 was significantly and dose-dependently reduced, resulting in the restoration of Th17/Treg balance. Moreover, BXS also improved the feces Lactobacillus levels and manifested beneficial effects on Bacteroides. CONCLUSIONS: The findings of the present study suggest that BXS is curative in a mouse model of ulcerative colitis, and the underlying mechanism might involve disruption of the Th17 pathway and the induction of a Th17/Treg imbalance, as well as an the development of an opsonic effect on specific gut microbiota. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/27132718/The_potential_mechanism_of_Bawei_Xileisan_in_the_treatment_of_dextran_sulfate_sodium_induced_ulcerative_colitis_in_mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(16)30255-0 DB - PRIME DP - Unbound Medicine ER -