Tags

Type your tag names separated by a space and hit enter

Effect of dietary docosahexaenoic acid (DHA) in phospholipids or triglycerides on brain DHA uptake and accretion.
J Nutr Biochem. 2016 07; 33:91-102.JN

Abstract

Tracer studies suggest that phospholipid DHA (PL-DHA) more effectively targets the brain than triglyceride DHA (TAG-DHA), although the mechanism and whether this translates into higher brain DHA concentrations are not clear. Rats were gavaged with [U-(3)H]PL-DHA and [U-(3)H]TAG-DHA and blood sampled over 6h prior to collection of brain regions and other tissues. In another experiment, rats were supplemented for 4weeks with TAG-DHA (fish oil), PL-DHA (roe PL) or a mixture of both for comparison to a low-omega-3 diet. Brain regions and other tissues were collected, and blood was sampled weekly. DHA accretion rates were estimated using the balance method. [U-(3)H]PL-DHA rats had higher radioactivity in cerebellum, hippocampus and remainder of brain, with no differences in other tissues despite higher serum lipid radioactivity in [U-(3)H]TAG-DHA rats. TAG-DHA, PL-DHA or a mixture were equally effective at increasing brain DHA. There were no differences between DHA-supplemented groups in brain region, whole-body, or tissue DHA accretion rates except heart and serum TAG where the PL-DHA/TAG-DHA blend was higher than TAG-DHA. Apparent DHA β-oxidation was not different between DHA-supplemented groups. This indicates that more labeled DHA enters the brain when consumed as PL; however, this may not translate into higher brain DHA concentrations.

Authors+Show Affiliations

Department of Nutritional Sciences, University of Toronto, Toronto, Ontario, M5S3E2, Canada.Department of Nutritional Sciences, University of Toronto, Toronto, Ontario, M5S3E2, Canada.Department of Nutritional Sciences, University of Toronto, Toronto, Ontario, M5S3E2, Canada.Department of Nutritional Sciences, University of Toronto, Toronto, Ontario, M5S3E2, Canada.Department of Nutritional Sciences, University of Toronto, Toronto, Ontario, M5S3E2, Canada.Arctic Nutrition AS, NO-6155, Ørsta, Norway; Department of Food Science & Nutrition, University of Minnesota, St. Paul, MN, 55108-1038, USA.Department of Nutritional Sciences, University of Toronto, Toronto, Ontario, M5S3E2, Canada. Electronic address: richard.bazinet@utoronto.ca.

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27135386

Citation

Kitson, Alex P., et al. "Effect of Dietary Docosahexaenoic Acid (DHA) in Phospholipids or Triglycerides On Brain DHA Uptake and Accretion." The Journal of Nutritional Biochemistry, vol. 33, 2016, pp. 91-102.
Kitson AP, Metherel AH, Chen CT, et al. Effect of dietary docosahexaenoic acid (DHA) in phospholipids or triglycerides on brain DHA uptake and accretion. J Nutr Biochem. 2016;33:91-102.
Kitson, A. P., Metherel, A. H., Chen, C. T., Domenichiello, A. F., Trépanier, M. O., Berger, A., & Bazinet, R. P. (2016). Effect of dietary docosahexaenoic acid (DHA) in phospholipids or triglycerides on brain DHA uptake and accretion. The Journal of Nutritional Biochemistry, 33, 91-102. https://doi.org/10.1016/j.jnutbio.2016.02.009
Kitson AP, et al. Effect of Dietary Docosahexaenoic Acid (DHA) in Phospholipids or Triglycerides On Brain DHA Uptake and Accretion. J Nutr Biochem. 2016;33:91-102. PubMed PMID: 27135386.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of dietary docosahexaenoic acid (DHA) in phospholipids or triglycerides on brain DHA uptake and accretion. AU - Kitson,Alex P, AU - Metherel,Adam H, AU - Chen,Chuck T, AU - Domenichiello,Anthony F, AU - Trépanier,Marc-Olivier, AU - Berger,Alvin, AU - Bazinet,Richard P, Y1 - 2016/03/21/ PY - 2015/10/06/received PY - 2016/01/27/revised PY - 2016/02/11/accepted PY - 2016/5/3/entrez PY - 2016/5/3/pubmed PY - 2017/11/29/medline KW - Brain KW - Docosahexaenoic acid KW - Phospholipid KW - Serum KW - Triacylglycerol KW - Uptake SP - 91 EP - 102 JF - The Journal of nutritional biochemistry JO - J Nutr Biochem VL - 33 N2 - Tracer studies suggest that phospholipid DHA (PL-DHA) more effectively targets the brain than triglyceride DHA (TAG-DHA), although the mechanism and whether this translates into higher brain DHA concentrations are not clear. Rats were gavaged with [U-(3)H]PL-DHA and [U-(3)H]TAG-DHA and blood sampled over 6h prior to collection of brain regions and other tissues. In another experiment, rats were supplemented for 4weeks with TAG-DHA (fish oil), PL-DHA (roe PL) or a mixture of both for comparison to a low-omega-3 diet. Brain regions and other tissues were collected, and blood was sampled weekly. DHA accretion rates were estimated using the balance method. [U-(3)H]PL-DHA rats had higher radioactivity in cerebellum, hippocampus and remainder of brain, with no differences in other tissues despite higher serum lipid radioactivity in [U-(3)H]TAG-DHA rats. TAG-DHA, PL-DHA or a mixture were equally effective at increasing brain DHA. There were no differences between DHA-supplemented groups in brain region, whole-body, or tissue DHA accretion rates except heart and serum TAG where the PL-DHA/TAG-DHA blend was higher than TAG-DHA. Apparent DHA β-oxidation was not different between DHA-supplemented groups. This indicates that more labeled DHA enters the brain when consumed as PL; however, this may not translate into higher brain DHA concentrations. SN - 1873-4847 UR - https://www.unboundmedicine.com/medline/citation/27135386/Effect_of_dietary_docosahexaenoic_acid__DHA__in_phospholipids_or_triglycerides_on_brain_DHA_uptake_and_accretion_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0955-2863(16)30016-X DB - PRIME DP - Unbound Medicine ER -