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Passiflora edulis peel intake improves insulin sensitivity, increasing incretins and hypothalamic satietogenic neuropeptide in rats on a high-fat diet.
Nutrition. 2016 Jul-Aug; 32(7-8):863-70.N

Abstract

OBJECTIVE

This study aimed to investigate the effect of Passiflora edulis peel flour (PEPF) intake on hypothalamic neuropeptides messenger RNA expression, insulin sensitivity, and other metabolic parameters in Sprague-Dawley rats fed a high-fat (HF) diet.

METHODS

Sprague-Dawley rats were divided in 3 groups: a control group, fed on a normal fat diet; a HF group, fed on a high-fat diet (35% fat [w/w]); and a high-fat Passiflora flour (HFPF) group, fed on a HF diet containing PEPF. The rats from the HFPF group as well as the HF group were kept on an HF diet for the first 4 wk to induce metabolic conditions related to obesity. Then the HFPF group was switched to a HF diet containing PEPF for additional 6 wk. Other groups were kept on normal-fat and HF diet without addition of PEPF during the whole period of experiment. The glucose tolerance and insulin sensitivity were evaluated through the glucose tolerance test (GTT) and the insulin tolerance test (ITT). Gut hormones and adipokines were measured through an immunoassay. The hypothalamic neuropeptides expression was assessed by real-time polymerase chain reaction.

RESULTS

The PEPF intake increased the hypothalamic cocaine- and amphetamine-regulated transcript expression (CART) (P < 0.05), counteracted cumulative body weight gain (P < 0.001), decreased adiposity (P < 0.05) and leptin level (P < 0.01), whereas increased adiponectin (P < 0.01), glucose-dependent insulinotropic polypeptide (P < 0.01), and glucagon-like peptide-1 (GLP-1) (P < 0.001) improved the insulin sensitivity in diet-induced obesity rats by increasing the kITT (glucose disappearance rate) (P < 0.01), which was calculated during the ITT. Other gut hormones (peptide tyrosine tyrosine, pancreatic polypeptide, and amylin) and interleukins (IL) (IL-6, tumor necrosis factor-α, IL-1β, and monocyte chemoattractant protein-1) were not changed by the PEPF intake.

CONCLUSION

Our findings provide a further understanding of how the PEPF works as a dietary component to improve glucose homeostasis and demonstrate a molecular mechanism that may increase satiety by PEPF in diet-induced obesity.

Authors+Show Affiliations

School of Food Engineering, University of Campinas, São Paulo, Brazil.School of Food Engineering, University of Campinas, São Paulo, Brazil.School of Food Engineering, University of Campinas, São Paulo, Brazil.Laboratory of Cell Signaling, Faculty of Medical Sciences, University of Campinas, São Paulo, Brazil.Laboratory of Cell Signaling, Faculty of Medical Sciences, University of Campinas, São Paulo, Brazil.School of Food Engineering, University of Campinas, São Paulo, Brazil. Electronic address: mario@fea.unicamp.br.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27138107

Citation

Lima, Glaucia Carielo, et al. "Passiflora Edulis Peel Intake Improves Insulin Sensitivity, Increasing Incretins and Hypothalamic Satietogenic Neuropeptide in Rats On a High-fat Diet." Nutrition (Burbank, Los Angeles County, Calif.), vol. 32, no. 7-8, 2016, pp. 863-70.
Lima GC, Vuolo MM, Batista ÂG, et al. Passiflora edulis peel intake improves insulin sensitivity, increasing incretins and hypothalamic satietogenic neuropeptide in rats on a high-fat diet. Nutrition. 2016;32(7-8):863-70.
Lima, G. C., Vuolo, M. M., Batista, Â. G., Dragano, N. R., Solon, C., & Maróstica Junior, M. R. (2016). Passiflora edulis peel intake improves insulin sensitivity, increasing incretins and hypothalamic satietogenic neuropeptide in rats on a high-fat diet. Nutrition (Burbank, Los Angeles County, Calif.), 32(7-8), 863-70. https://doi.org/10.1016/j.nut.2016.01.014
Lima GC, et al. Passiflora Edulis Peel Intake Improves Insulin Sensitivity, Increasing Incretins and Hypothalamic Satietogenic Neuropeptide in Rats On a High-fat Diet. Nutrition. 2016 Jul-Aug;32(7-8):863-70. PubMed PMID: 27138107.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Passiflora edulis peel intake improves insulin sensitivity, increasing incretins and hypothalamic satietogenic neuropeptide in rats on a high-fat diet. AU - Lima,Glaucia Carielo, AU - Vuolo,Milena Morandi, AU - Batista,Ângela Giovana, AU - Dragano,Nathália R V, AU - Solon,Carina, AU - Maróstica Junior,Mário Roberto, Y1 - 2016/01/30/ PY - 2015/08/13/received PY - 2015/12/11/revised PY - 2016/01/20/accepted PY - 2016/5/4/entrez PY - 2016/5/4/pubmed PY - 2017/4/1/medline KW - GLP-1 KW - Insulin resistance KW - Neuropeptides KW - Obesity KW - Passiflora edulis KW - Pectin SP - 863 EP - 70 JF - Nutrition (Burbank, Los Angeles County, Calif.) JO - Nutrition VL - 32 IS - 7-8 N2 - OBJECTIVE: This study aimed to investigate the effect of Passiflora edulis peel flour (PEPF) intake on hypothalamic neuropeptides messenger RNA expression, insulin sensitivity, and other metabolic parameters in Sprague-Dawley rats fed a high-fat (HF) diet. METHODS: Sprague-Dawley rats were divided in 3 groups: a control group, fed on a normal fat diet; a HF group, fed on a high-fat diet (35% fat [w/w]); and a high-fat Passiflora flour (HFPF) group, fed on a HF diet containing PEPF. The rats from the HFPF group as well as the HF group were kept on an HF diet for the first 4 wk to induce metabolic conditions related to obesity. Then the HFPF group was switched to a HF diet containing PEPF for additional 6 wk. Other groups were kept on normal-fat and HF diet without addition of PEPF during the whole period of experiment. The glucose tolerance and insulin sensitivity were evaluated through the glucose tolerance test (GTT) and the insulin tolerance test (ITT). Gut hormones and adipokines were measured through an immunoassay. The hypothalamic neuropeptides expression was assessed by real-time polymerase chain reaction. RESULTS: The PEPF intake increased the hypothalamic cocaine- and amphetamine-regulated transcript expression (CART) (P < 0.05), counteracted cumulative body weight gain (P < 0.001), decreased adiposity (P < 0.05) and leptin level (P < 0.01), whereas increased adiponectin (P < 0.01), glucose-dependent insulinotropic polypeptide (P < 0.01), and glucagon-like peptide-1 (GLP-1) (P < 0.001) improved the insulin sensitivity in diet-induced obesity rats by increasing the kITT (glucose disappearance rate) (P < 0.01), which was calculated during the ITT. Other gut hormones (peptide tyrosine tyrosine, pancreatic polypeptide, and amylin) and interleukins (IL) (IL-6, tumor necrosis factor-α, IL-1β, and monocyte chemoattractant protein-1) were not changed by the PEPF intake. CONCLUSION: Our findings provide a further understanding of how the PEPF works as a dietary component to improve glucose homeostasis and demonstrate a molecular mechanism that may increase satiety by PEPF in diet-induced obesity. SN - 1873-1244 UR - https://www.unboundmedicine.com/medline/citation/27138107/Passiflora_edulis_peel_intake_improves_insulin_sensitivity_increasing_incretins_and_hypothalamic_satietogenic_neuropeptide_in_rats_on_a_high_fat_diet_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0899-9007(16)00047-2 DB - PRIME DP - Unbound Medicine ER -