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Protective role of n6/n3 PUFA supplementation with varying DHA/EPA ratios against atherosclerosis in mice.
J Nutr Biochem 2016; 32:171-80JN

Abstract

The effects of n3 polyunsaturated fatty acids (PUFA) on cardiovascular disease are controversial. We currently explored the effects of various ratios of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) on high-fat-induced atherosclerosis. In model apoE(-/-) mice, high-fat diets (HFD) were partially replaced with fish and algal oils (DHA/EPA 2:1, 1:1 and 1:2) and/or plant oils enriched in linoleic and alpha-linolenic acids with an n6/n3 ratio of 4:1. PUFA supplementation significantly reduced the atherosclerotic plaque area, serum lipid profile, inflammatory response, aortic ROS production, proinflammatory factors and scavenger receptor expression as compared to those in the HFD group. However, plant oils did not have a significant effect on the following: serum HDL-C level; aortic ABCA1, ABCG1 and LAL mRNA expression; and CD36 and LOX-1 protein expression. Compared to the plant-oil-treated group, the DHA/EPA 1:1 group had a smaller atherosclerotic plaque area, higher serum HDL-C levels and lesser CD36 and MSR-1 mRNA expression; the DHA/EPA 2:1 group had lower serum TC, LDL-C and TNF-α levels and lower aortic ROS levels. Our study suggested that n3 PUFA from animals had more potent atheroprotective effects than that from plants. Supplementation involving higher DHA/EPA ratios and an n6/n3 ratio of 4:1 was beneficial for reducing serum "bad cholesterol" and a 1:1 DHA/EPA ratio with an n6/n3 ratio of 4:1 was beneficial for improving serum "good cholesterol" and inhibiting ox-LDL uptake. Our results suggest that achieving an n6/n3 ratio of 4:1 in the diet is also important in addition to having an optimal DHA/EPA ratio.

Authors+Show Affiliations

College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, 430023, People's Republic of China; Hubei Collaborative Innovation Center for Processing of Agricultural Products, Wuhan Polytechnic University, Wuhan, 430023, People's Republic of China.College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, 430023, People's Republic of China; Hubei Collaborative Innovation Center for Processing of Agricultural Products, Wuhan Polytechnic University, Wuhan, 430023, People's Republic of China.College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, 430023, People's Republic of China; Hubei Collaborative Innovation Center for Processing of Agricultural Products, Wuhan Polytechnic University, Wuhan, 430023, People's Republic of China.College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, 430023, People's Republic of China; Hubei Collaborative Innovation Center for Processing of Agricultural Products, Wuhan Polytechnic University, Wuhan, 430023, People's Republic of China.College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, 430023, People's Republic of China; Hubei Collaborative Innovation Center for Processing of Agricultural Products, Wuhan Polytechnic University, Wuhan, 430023, People's Republic of China.College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, 430023, People's Republic of China; Hubei Collaborative Innovation Center for Processing of Agricultural Products, Wuhan Polytechnic University, Wuhan, 430023, People's Republic of China.Hubei Collaborative Innovation Center for Processing of Agricultural Products, Wuhan Polytechnic University, Wuhan, 430023, People's Republic of China; School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, 430023, People's Republic of China.Department of Nutrition and Food Hygiene, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, People's Republic of China.College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, 430023, People's Republic of China; Hubei Collaborative Innovation Center for Processing of Agricultural Products, Wuhan Polytechnic University, Wuhan, 430023, People's Republic of China.College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, 430023, People's Republic of China; Hubei Collaborative Innovation Center for Processing of Agricultural Products, Wuhan Polytechnic University, Wuhan, 430023, People's Republic of China. Electronic address: gongwhpu@126.com.

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

27142749

Citation

Liu, Liang, et al. "Protective Role of N6/n3 PUFA Supplementation With Varying DHA/EPA Ratios Against Atherosclerosis in Mice." The Journal of Nutritional Biochemistry, vol. 32, 2016, pp. 171-80.
Liu L, Hu Q, Wu H, et al. Protective role of n6/n3 PUFA supplementation with varying DHA/EPA ratios against atherosclerosis in mice. J Nutr Biochem. 2016;32:171-80.
Liu, L., Hu, Q., Wu, H., Xue, Y., Cai, L., Fang, M., ... Gong, Z. (2016). Protective role of n6/n3 PUFA supplementation with varying DHA/EPA ratios against atherosclerosis in mice. The Journal of Nutritional Biochemistry, 32, pp. 171-80. doi:10.1016/j.jnutbio.2016.02.010.
Liu L, et al. Protective Role of N6/n3 PUFA Supplementation With Varying DHA/EPA Ratios Against Atherosclerosis in Mice. J Nutr Biochem. 2016;32:171-80. PubMed PMID: 27142749.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective role of n6/n3 PUFA supplementation with varying DHA/EPA ratios against atherosclerosis in mice. AU - Liu,Liang, AU - Hu,Qinling, AU - Wu,Huihui, AU - Xue,Yihong, AU - Cai,Liang, AU - Fang,Min, AU - Liu,Zhiguo, AU - Yao,Ping, AU - Wu,Yongning, AU - Gong,Zhiyong, Y1 - 2016/03/21/ PY - 2015/10/12/received PY - 2016/01/16/revised PY - 2016/02/08/accepted PY - 2016/5/5/entrez PY - 2016/5/5/pubmed PY - 2017/10/11/medline KW - Alpha-linolenic acid KW - Atherosclerosis KW - DHA/EPA KW - Docosahexaenoic acid KW - Eicosapentaenoic acid KW - n3 PUFA SP - 171 EP - 80 JF - The Journal of nutritional biochemistry JO - J. Nutr. Biochem. VL - 32 N2 - The effects of n3 polyunsaturated fatty acids (PUFA) on cardiovascular disease are controversial. We currently explored the effects of various ratios of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) on high-fat-induced atherosclerosis. In model apoE(-/-) mice, high-fat diets (HFD) were partially replaced with fish and algal oils (DHA/EPA 2:1, 1:1 and 1:2) and/or plant oils enriched in linoleic and alpha-linolenic acids with an n6/n3 ratio of 4:1. PUFA supplementation significantly reduced the atherosclerotic plaque area, serum lipid profile, inflammatory response, aortic ROS production, proinflammatory factors and scavenger receptor expression as compared to those in the HFD group. However, plant oils did not have a significant effect on the following: serum HDL-C level; aortic ABCA1, ABCG1 and LAL mRNA expression; and CD36 and LOX-1 protein expression. Compared to the plant-oil-treated group, the DHA/EPA 1:1 group had a smaller atherosclerotic plaque area, higher serum HDL-C levels and lesser CD36 and MSR-1 mRNA expression; the DHA/EPA 2:1 group had lower serum TC, LDL-C and TNF-α levels and lower aortic ROS levels. Our study suggested that n3 PUFA from animals had more potent atheroprotective effects than that from plants. Supplementation involving higher DHA/EPA ratios and an n6/n3 ratio of 4:1 was beneficial for reducing serum "bad cholesterol" and a 1:1 DHA/EPA ratio with an n6/n3 ratio of 4:1 was beneficial for improving serum "good cholesterol" and inhibiting ox-LDL uptake. Our results suggest that achieving an n6/n3 ratio of 4:1 in the diet is also important in addition to having an optimal DHA/EPA ratio. SN - 1873-4847 UR - https://www.unboundmedicine.com/medline/citation/27142749/Protective_role_of_n6/n3_PUFA_supplementation_with_varying_DHA/EPA_ratios_against_atherosclerosis_in_mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0955-2863(16)30017-1 DB - PRIME DP - Unbound Medicine ER -