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The contribution of the cytoplasmic retrieval signal of severe acute respiratory syndrome coronavirus to intracellular accumulation of S proteins and incorporation of S protein into virus-like particles.
J Gen Virol. 2016 08; 97(8):1853-1864.JG

Abstract

The cytoplasmic tails of some coronavirus (CoV) spike (S) proteins contain an endoplasmic reticulum retrieval signal (ERRS) that can retrieve S proteins from the Golgi to the endoplasmic reticulum (ER); this process is thought to accumulate S proteins at the CoV budding site, the ER-Golgi intermediate compartment (ERGIC), and to facilitate S protein incorporation into virions. However, we showed previously that porcine epidemic diarrhoea CoV S proteins lacking the ERRS were efficiently incorporated into virions, similar to the original virus. Thus, the precise role of the ERRS in virus assembly remains unclear. Here, the roles of the S protein ERRS in severe acute respiratory syndrome CoV (SARS-CoV) intracellular trafficking and S incorporation into virus-like particles (VLPs) are described. Intracellular trafficking and indirect immunofluorescence analysis suggested that when M protein was present, wild-type S protein (wtS) could be retained in the pre- and post-medial Golgi compartments intracellularly and co-localized with M protein in the Golgi. In contrast, mutant S protein lacking the ERRS was distributed throughout the ER and only partially co-localized with M protein. Moreover, the intracellular accumulation of mutant S protein, particularly at the post-medial Golgi compartment, was significantly reduced compared with wtS. A VLP assay suggested that wtS that reached the post-medial compartment could be returned to the ERGIC for subsequent incorporation into VLPs, while mutant S protein could not. These results suggest that the ERRS of SARS-CoV contributes to intracellular S protein accumulation specifically in the post-medial Golgi compartment and to S protein incorporation into VLPs.

Authors+Show Affiliations

Laboratory of Virology and Viral Infections, Faculty of Veterinary Medicine, Nippon Veterinary and Life Science University, 1-7-1 Kyonan-cho, Musashino, Tokyo 180-8602, Japan.Department of Microbiology and Immunology, The University of Texas Medical Branch at Galveston, Galveston, TX 77555-1019, USA.Laboratory of Acute Respiratory Viral Diseases and Cytokines, Department of Virology III, National Institute of Infectious Diseases, Gakuen 4-7-1 Musashimurayama, Tokyo 208-0011, Japan.Department of Microbiology and Immunology, The University of Texas Medical Branch at Galveston, Galveston, TX 77555-1019, USA.Laboratory of Virology and Viral Infections, Faculty of Veterinary Medicine, Nippon Veterinary and Life Science University, 1-7-1 Kyonan-cho, Musashino, Tokyo 180-8602, Japan.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27145752

Citation

Ujike, Makoto, et al. "The Contribution of the Cytoplasmic Retrieval Signal of Severe Acute Respiratory Syndrome Coronavirus to Intracellular Accumulation of S Proteins and Incorporation of S Protein Into Virus-like Particles." The Journal of General Virology, vol. 97, no. 8, 2016, pp. 1853-1864.
Ujike M, Huang C, Shirato K, et al. The contribution of the cytoplasmic retrieval signal of severe acute respiratory syndrome coronavirus to intracellular accumulation of S proteins and incorporation of S protein into virus-like particles. J Gen Virol. 2016;97(8):1853-1864.
Ujike, M., Huang, C., Shirato, K., Makino, S., & Taguchi, F. (2016). The contribution of the cytoplasmic retrieval signal of severe acute respiratory syndrome coronavirus to intracellular accumulation of S proteins and incorporation of S protein into virus-like particles. The Journal of General Virology, 97(8), 1853-1864. https://doi.org/10.1099/jgv.0.000494
Ujike M, et al. The Contribution of the Cytoplasmic Retrieval Signal of Severe Acute Respiratory Syndrome Coronavirus to Intracellular Accumulation of S Proteins and Incorporation of S Protein Into Virus-like Particles. J Gen Virol. 2016;97(8):1853-1864. PubMed PMID: 27145752.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The contribution of the cytoplasmic retrieval signal of severe acute respiratory syndrome coronavirus to intracellular accumulation of S proteins and incorporation of S protein into virus-like particles. AU - Ujike,Makoto, AU - Huang,Cheng, AU - Shirato,Kazuya, AU - Makino,Shinji, AU - Taguchi,Fumihiro, Y1 - 2016/05/04/ PY - 2016/5/6/entrez PY - 2016/5/6/pubmed PY - 2017/5/10/medline SP - 1853 EP - 1864 JF - The Journal of general virology JO - J Gen Virol VL - 97 IS - 8 N2 - The cytoplasmic tails of some coronavirus (CoV) spike (S) proteins contain an endoplasmic reticulum retrieval signal (ERRS) that can retrieve S proteins from the Golgi to the endoplasmic reticulum (ER); this process is thought to accumulate S proteins at the CoV budding site, the ER-Golgi intermediate compartment (ERGIC), and to facilitate S protein incorporation into virions. However, we showed previously that porcine epidemic diarrhoea CoV S proteins lacking the ERRS were efficiently incorporated into virions, similar to the original virus. Thus, the precise role of the ERRS in virus assembly remains unclear. Here, the roles of the S protein ERRS in severe acute respiratory syndrome CoV (SARS-CoV) intracellular trafficking and S incorporation into virus-like particles (VLPs) are described. Intracellular trafficking and indirect immunofluorescence analysis suggested that when M protein was present, wild-type S protein (wtS) could be retained in the pre- and post-medial Golgi compartments intracellularly and co-localized with M protein in the Golgi. In contrast, mutant S protein lacking the ERRS was distributed throughout the ER and only partially co-localized with M protein. Moreover, the intracellular accumulation of mutant S protein, particularly at the post-medial Golgi compartment, was significantly reduced compared with wtS. A VLP assay suggested that wtS that reached the post-medial compartment could be returned to the ERGIC for subsequent incorporation into VLPs, while mutant S protein could not. These results suggest that the ERRS of SARS-CoV contributes to intracellular S protein accumulation specifically in the post-medial Golgi compartment and to S protein incorporation into VLPs. SN - 1465-2099 UR - https://www.unboundmedicine.com/medline/citation/27145752/The_contribution_of_the_cytoplasmic_retrieval_signal_of_severe_acute_respiratory_syndrome_coronavirus_to_intracellular_accumulation_of_S_proteins_and_incorporation_of_S_protein_into_virus_like_particles_ L2 - http://jgv.microbiologyresearch.org/pubmed/content/journal/jgv/10.1099/jgv.0.000494 DB - PRIME DP - Unbound Medicine ER -