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The prolonged effect of glucagon-like peptide 2 pretreatment on growth performance and intestinal development of weaned piglets.
J Anim Sci Biotechnol. 2016; 7:28.JA

Abstract

BACKGROUND

Glucagon-like peptide 2 (GLP-2) is a potent epithelium-specific intestinal growth factor. The aim of this study was to demonstrate the prolonged effect of GLP-2 on the growth performance of weaned piglets. Forty piglets weaned at the age of 28 d with an average BW of 6.8 ± 0.4 kg were assigned to four treatments: (i) non-challenged control; (ii) LPS-challenged control; (iii) LPS + low GLP-2; and (iv) LPS + high GLP-2. Piglets in groups (i), (ii), and (iv) were s.c. injected with PBS supplemented with human [Gly2]GLP-21-34 at doses of 0, 2 and 10 nmol/kg BW per day for seven consecutive days. BW, gain:feed ratio (G:F), and plasma GLP-2 levels were determined on d 0, 7, and 14 after weaning. Piglets were challenged with i.p. administration of Escherichia coli lipopolysaccharide (LPS) at a dose of 100 μg/kg on d 14 to induce intestinal damage. Twenty-four hours later, intestinal tract samples were collected to assess intestinal morphology and quantify enzyme activity.

RESULTS

Plasma GLP-2 levels decreased after weaning, but in the high GLP-2 group, plasma GLP-2 was maintained on d 7 and even increased to a level higher than the preweaning level on d 14 (P < 0.05). High GLP-2 treatment significantly increased the duodenal, jejunal and ileal weight, as well as the gross weight of the small intestine (SI), and the SI weight index (P < 0.05). LPS caused villous atrophy and disrupted intestinal morphology in the duodenum, jejunum and ileum. GLP-2 also significantly increased the villus height and the villus height/crypt depth ratio (VCR) of the duodenum, jejunum, and ileum (P < 0.05). Histological examination revealed that in GLP-2-treated groups, the integrity of the villus was maintained, and the villus was protected against LPS-induced damage. GLP-2 significantly increased the activity of alkaline phosphatase (AKP), γ-glutamyltranspeptidase (γ-GT), and pancreatic lipase in the duodenum and jejunum (P < 0.05). GLP-2 treatment also significantly increased the average daily gain (ADG) and G:F of piglets at 0 to 7, 7 to 14, as well as 0 to14 d (P < 0.05), resulting in a significant increase of final BW in high GLP-2 pigs (P = 0.016).

CONCLUSIONS

Exogenous GLP-2 improved the growth of weaned piglets and protected them against LPS-induced intestinal damage. These effects may be due to the ability of GLP-2 to promote the secretion of endogenous GLP-2 to stimulate the small intestinal development.

Authors+Show Affiliations

Animal Nutrition Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130 China.Animal Nutrition Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130 China.Animal Nutrition Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130 China.College of Animal Medicine, Sichuan Agricultural University, Chengdu, Sichuan 611130 China.Animal Nutrition Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130 China.Animal Nutrition Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130 China.Animal Nutrition Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130 China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27148449

Citation

Deng, Qiu Hong, et al. "The Prolonged Effect of Glucagon-like Peptide 2 Pretreatment On Growth Performance and Intestinal Development of Weaned Piglets." Journal of Animal Science and Biotechnology, vol. 7, 2016, p. 28.
Deng QH, Jia G, Zhao H, et al. The prolonged effect of glucagon-like peptide 2 pretreatment on growth performance and intestinal development of weaned piglets. Journal of animal science and biotechnology. 2016;7:28.
Deng, Q. H., Jia, G., Zhao, H., Chen, Z. L., Chen, X. L., Liu, G. M., & Wang, K. N. (2016). The prolonged effect of glucagon-like peptide 2 pretreatment on growth performance and intestinal development of weaned piglets. Journal of Animal Science and Biotechnology, 7, 28. https://doi.org/10.1186/s40104-016-0087-7
Deng QH, et al. The Prolonged Effect of Glucagon-like Peptide 2 Pretreatment On Growth Performance and Intestinal Development of Weaned Piglets. Journal of animal science and biotechnology. 2016;7:28. PubMed PMID: 27148449.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The prolonged effect of glucagon-like peptide 2 pretreatment on growth performance and intestinal development of weaned piglets. AU - Deng,Qiu Hong, AU - Jia,Gang, AU - Zhao,Hua, AU - Chen,Zheng Li, AU - Chen,Xiao Ling, AU - Liu,Guang Mang, AU - Wang,Kang Ning, Y1 - 2016/05/04/ PY - 2015/11/07/received PY - 2016/04/19/accepted PY - 2016/5/6/entrez PY - 2016/5/6/pubmed PY - 2016/5/6/medline KW - Escherichia coli lipopolysaccharide KW - Glucagon-like peptide-2 KW - Growth performance KW - Intestinal enzymes KW - Small intestinal morphology KW - Weaned piglets SP - 28 EP - 28 JF - Journal of animal science and biotechnology VL - 7 N2 - BACKGROUND: Glucagon-like peptide 2 (GLP-2) is a potent epithelium-specific intestinal growth factor. The aim of this study was to demonstrate the prolonged effect of GLP-2 on the growth performance of weaned piglets. Forty piglets weaned at the age of 28 d with an average BW of 6.8 ± 0.4 kg were assigned to four treatments: (i) non-challenged control; (ii) LPS-challenged control; (iii) LPS + low GLP-2; and (iv) LPS + high GLP-2. Piglets in groups (i), (ii), and (iv) were s.c. injected with PBS supplemented with human [Gly2]GLP-21-34 at doses of 0, 2 and 10 nmol/kg BW per day for seven consecutive days. BW, gain:feed ratio (G:F), and plasma GLP-2 levels were determined on d 0, 7, and 14 after weaning. Piglets were challenged with i.p. administration of Escherichia coli lipopolysaccharide (LPS) at a dose of 100 μg/kg on d 14 to induce intestinal damage. Twenty-four hours later, intestinal tract samples were collected to assess intestinal morphology and quantify enzyme activity. RESULTS: Plasma GLP-2 levels decreased after weaning, but in the high GLP-2 group, plasma GLP-2 was maintained on d 7 and even increased to a level higher than the preweaning level on d 14 (P < 0.05). High GLP-2 treatment significantly increased the duodenal, jejunal and ileal weight, as well as the gross weight of the small intestine (SI), and the SI weight index (P < 0.05). LPS caused villous atrophy and disrupted intestinal morphology in the duodenum, jejunum and ileum. GLP-2 also significantly increased the villus height and the villus height/crypt depth ratio (VCR) of the duodenum, jejunum, and ileum (P < 0.05). Histological examination revealed that in GLP-2-treated groups, the integrity of the villus was maintained, and the villus was protected against LPS-induced damage. GLP-2 significantly increased the activity of alkaline phosphatase (AKP), γ-glutamyltranspeptidase (γ-GT), and pancreatic lipase in the duodenum and jejunum (P < 0.05). GLP-2 treatment also significantly increased the average daily gain (ADG) and G:F of piglets at 0 to 7, 7 to 14, as well as 0 to14 d (P < 0.05), resulting in a significant increase of final BW in high GLP-2 pigs (P = 0.016). CONCLUSIONS: Exogenous GLP-2 improved the growth of weaned piglets and protected them against LPS-induced intestinal damage. These effects may be due to the ability of GLP-2 to promote the secretion of endogenous GLP-2 to stimulate the small intestinal development. SN - 1674-9782 UR - https://www.unboundmedicine.com/medline/citation/27148449/The_prolonged_effect_of_glucagon_like_peptide_2_pretreatment_on_growth_performance_and_intestinal_development_of_weaned_piglets_ L2 - https://jasbsci.biomedcentral.com/articles/10.1186/s40104-016-0087-7 DB - PRIME DP - Unbound Medicine ER -
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